MAGI3 (NM_152900) Human Tagged ORF Clone

SKU RC218925

MAGI3 (Myc-DDK-tagged)-Human membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: MAGI3
• Synonyms: dJ730K3.2; MAGI-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218925 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCGAAGACGCTGAAGAAGAAGAAGCACTGGCTCAGCAAGGTGCAGGAGTGCGCCGTGTCCTGGGCCG; GGCCCCCGGGCGACTTCGGCGCGGAGATCCGCGGTGGCGCGGAGCGTGGCGAGTTCCCCTACCTGGGGCG; GCTCCGCGAGGAGCCCGGCGGGGGCACCTGCTGCGTCGTCTCGGGCAAGGCGCCCAGCCCAGGCGATGTG; CTGCTGGAGGTAAACGGGACGCCTGTCAGCGGGCTCACCAACCGGGACACCCTGGCTGTCATCCGCCACT; TCCGCGAGCCCATCCGTCTCAAGACTGTGAAACCAGGCAAAGTCATTAATAAAGATTTGCGGCATTACCT; AAGTCTTCAGTTTCAAAAAGGATCAATTGACCACAAACTGCAGCAAGTGATCAGAGATAATCTCTACTTG; AGAACCATTCCATGCACTACAAGGGCCCCCAGGGATGGAGAAGTACCAGGAGTGGATTATAATTTCATTT; CCGTTGAACAGTTCAAAGCACTGGAAGAGAGTGGAGCATTGTTAGAAAGTGGGACATATGATGGAAACTT; CTATGGAACTCCCAAGCCTCCAGCAGAACCCAGCCCTTTTCAGCCAGATCCAGTTGATCAAGTCCTCTTT; GATAATGAGTTTGATGCAGAATCTCAAAGAAAACGAACGACATCTGTCAGCAAGATGGAAAGAATGGATA; GCTCTCTTCCTGAAGAGGAAGAAGATGAGGACAAGGAAGCTATTAATGGCAGTGGAAACGCAGAAAACAG; AGAGAGGCATTCTGAGTCATCTGACTGGATGAAGACTGTTCCAAGTTACAACCAAACAAATAGCTCCATG; GACTTTAGAAATTATATGATGAGAGATGAGACTCTGGAACCACTGCCCAAAAACTGGGAAATGGCCTACA; CTGACACAGGGATGATCTACTTCATTGACCACAATACCAAGACAACCACCTGGTTGGATCCTCGTCTTTG; TAAGAAAGCCAAAGCCCCTGAAGACTGTGAAGATGGAGAGCTTCCTTATGGCTGGGAGAAAATAGAGGAC; CCTCAGTATGGGACATACTATGTTGATCACCTTAACCAGAAAACCCAGTTTGAAAATCCAGTGGAGGAAG; CCAAAAGGAAAAAGCAGTTAGGACAGGTTGAAATTGGGTCTTCAAAACCAGATATGGAAAAATCACACTT; CACAAGAGATCCATCCCAGCTTAAAGGTGTCCTTGTTCGAGCATCACTGAAAAAAAGCACAATGGGATTT; GGTTTTACTATTATTGGTGGAGATAGACCTGATGAGTTCCTACAAGTGAAAAATGTGCTGAAAGATGGTC; CCGCAGCTCAGGATGGGAAAATTGCACCAGGCGATGTTATTGTAGACATCAATGGCAACTGTGTCCTCGG; TCACACTCATGCAGATGTTGTCCAGATGTTTCAATTGGTACCTGTCAATCAGTATGTAAACCTCACTTTA; TGTCGTGGTTATCCACTTCCTGATGACAGTGAAGATCCTGTTGTGGACATTGTTGCTGCTACCCCTGTCA; TCAATGGACAGTCATTAACCAAGGGAGAGACTTGCATGAATCCTCAGGATTTTAAGCCAGGAGCAATGGT; TCTGGAGCAGAATGGAAAATCGGGACACACTTTGACTGGTGATGGTCTCAATGGACCATCAGATGCAAGT; GAGCAGAGAGTATCCATGGCATCGTCAGGCAGCTCCCAGCCTGAACTAGTGACTATCCCTTTGATTAAGG; GCCCTAAAGGGTTTGGGTTTGCAATTGCTGACAGCCCTACTGGACAGAAGGTGAAAATGATACTGGATAG; TCAGTGGTGTCAAGGCCTTCAGAAAGGAGATATAATTAAGGAAATATACCATCAAAATGTGCAGAATTTA; ACACATCTCCAAGTGGTAGAGGTGCTAAAGCAGTTTCCAGTAGGTGCTGATGTACCATTGCTTATCTTAA; GAGGAGGTCCTCCTTCACCAACCAAAACTGCCAAAATGAAAACAGATAAAAAGGAAAATGCAGGAAGTTT; GGAGGCCATAAATGAGCCTATTCCTCAGCCTATGCCTTTTCCACCGAGCATTATCAGGTCAGGATCCCCA; AAATTGGATCCTTCTGAGGTCTACCTGAAATCTAAGACTTTATATGAAGATAAACCACCAAACACCAAAG; ATTTGGATGTTTTTCTTCGAAAACAAGAGTCAGGGTTTGGCTTCAGGGTGCTAGGAGGAGATGGACCTGA; CCAGTCTATATATATTGGGGCTATTATTCCCCTGGGAGCAGCTGAGAAAGATGGTCGGCTCCGCGCAGCT; GATGAACTAATGTGCATTGATGGAATTCCTGTTAAAGGGAAATCACACAAACAAGTCTTGGACCTCATGA; CAACTGCTGCTCGAAATGGCCATGTGTTACTAACTGTCAGACGGAAGATCTTCTATGGAGAAAAACAACC; CGAGGACGACAGCTCTCAGGCCTTCATTTCAACACAGAATGGATCTCCCCGCCTGAACCGGGCAGAGGTC; CCAGCCAGGCCTGCACCCCAGGAGCCCTATGATGTTGTCTTGCAACGAAAAGAAAATGAAGGATTTGGCT; TTGTCATCCTCACCTCCAAAAACAAACCACCTCCAGGAGTTATTCCTCATAAAATTGGCCGAGTCATAGA; AGGAAGTCCGGCTGACCGCTGTGGAAAACTGAAAGTTGGAGATCATATCTCTGCAGTGAATGGGCAGTCC; ATTGTTGAACTGTCTCATGATAACATTGTTCAGCTGATCAAAGATGCTGGTGTCACCGTCACACTAACGG; TCATTGCTGAAGAAGAGCATCATGGTCCACCATCAGGAACAAACTCAGCCAGGCAAAGCCCAGCCCTGCA; GCACAGGCCCATGGGACAGTCACAGGCCAACCACATACCTGGGGACAGAAGTGCCCTAGAAGGTGAAATT; GGAAAAGATGTCTCCACTTCTTACAGACATTCTTGGTCAGACCACAAGCACCTTGCACAGCCTGACACCG; CAGTAATTTCAGTTGTAGGCAGTCGGCACAATCAGAACCTTGGTTGTTATCCAGTAGAGCTGGAGAGAGG; CCCCCGGGGCTTTGGATTCAGCCTCCGAGGGGGGAAGGAGTACAACATGGGGCTGTTCATCCTTCGTCTT; GCTGAAGATGGTCCTGCCATCAAAGATGGCAGAATTCATGTTGGTGACCAGATTGTTGAAATCAATGGGG; AACCTACACAAGGAATCACACATACTCGAGCAATTGAGCTCATTCAGGCTGGTGGAAATAAAGTTCTTCT; TCTTTTGAGGCCAGGAACTGGCTTGATACCTGACCATGGTTTGGCTCCTTCCGGTCTGTGCTCCTACGTG; AAACCCGAGCAACAT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218925 protein sequence; Red=Cloning site Green=Tags(s); ; MSKTLKKKKHWLSKVQECAVSWAGPPGDFGAEIRGGAERGEFPYLGRLREEPGGGTCCVVSGKAPSPGDV; LLEVNGTPVSGLTNRDTLAVIRHFREPIRLKTVKPGKVINKDLRHYLSLQFQKGSIDHKLQQVIRDNLYL; RTIPCTTRAPRDGEVPGVDYNFISVEQFKALEESGALLESGTYDGNFYGTPKPPAEPSPFQPDPVDQVLF; DNEFDAESQRKRTTSVSKMERMDSSLPEEEEDEDKEAINGSGNAENRERHSESSDWMKTVPSYNQTNSSM; DFRNYMMRDETLEPLPKNWEMAYTDTGMIYFIDHNTKTTTWLDPRLCKKAKAPEDCEDGELPYGWEKIED; PQYGTYYVDHLNQKTQFENPVEEAKRKKQLGQVEIGSSKPDMEKSHFTRDPSQLKGVLVRASLKKSTMGF; GFTIIGGDRPDEFLQVKNVLKDGPAAQDGKIAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYVNLTL; CRGYPLPDDSEDPVVDIVAATPVINGQSLTKGETCMNPQDFKPGAMVLEQNGKSGHTLTGDGLNGPSDAS; EQRVSMASSGSSQPELVTIPLIKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNVQNL; THLQVVEVLKQFPVGADVPLLILRGGPPSPTKTAKMKTDKKENAGSLEAINEPIPQPMPFPPSIIRSGSP; KLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRLRAA; DELMCIDGIPVKGKSHKQVLDLMTTAARNGHVLLTVRRKIFYGEKQPEDDSSQAFISTQNGSPRLNRAEV; PARPAPQEPYDVVLQRKENEGFGFVILTSKNKPPPGVIPHKIGRVIEGSPADRCGKLKVGDHISAVNGQS; IVELSHDNIVQLIKDAGVTVTLTVIAEEEHHGPPSGTNSARQSPALQHRPMGQSQANHIPGDRSALEGEI; GKDVSTSYRHSWSDHKHLAQPDTAVISVVGSRHNQNLGCYPVELERGPRGFGFSLRGGKEYNMGLFILRL; AEDGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTGLIPDHGLAPSGLCSYV; KPEQH; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152900
• ORF Size: 3375 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152900.3
• RefSeq Size: 7221 bp
• RefSeq ORF: 3378 bp
• Locus ID: 260425
• UniProt ID: Q5TCQ9
• Cytogenetics: 1p13.2
• Protein Families: Druggable Genome
• Protein Pathways: Tight junction
• MW: 123.3 kDa
• Summary: Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.[UniProtKB/Swiss-Prot Function]