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ALG11 (NM_001004127) Human Tagged ORF Clone

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SKU
RC218890
ALG11 (Myc-DDK-tagged)-Human asparagine-linked glycosylation 11, alpha-1,2-mannosyltransferase homolog (yeast) (ALG11), transcript variant A
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ALG11
Synonyms CDG1P; GT8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218890 representing NM_001004127
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCGGCGAAAGGAGCTGGTGCCTGTGCAAGTTGTTGAGGTTTTTTTATTCATTATTCTTCCCTG
GGCTCATTGTATGTGGAACTTTATGTGTGTGTTTGGTCATTGTCCTTTGGGGAATCAGACTGCTGCTACA
GAGAAAGAAAAAATTAGTGTCAACTAGCAGAAATGGGAAAAATCAAATGGTGATTGCATTTTTTCATCCA
TACTGCAATGCTGGTGGAGGAGGAGAAAGAGTTTTATGGTGTGCTTTAAGAGCCCTGCAGAAAAAGTATC
CTGAAGCAGTTTATGTTGTTTATACCGGCGATGTTAATGTCAACGGTCAACAGATACTAGAAGGTGCTTT
CAGAAGATTTAACATCAGATTAATTCACCCAGTGCAGTTTGTTTTTTTAAGGAAACGCTATCTTGTGGAA
GATTCACTGTATCCTCACTTCACACTGCTGGGCCAAAGTCTAGGATCCATTTTTCTTGGCTGGGAAGCTC
TAATGCAGTGTGTTCCTGATGTTTACATTGATTCAATGGGATACGCTTTTACGCTTCCTCTGTTTAAGTA
TATAGGGGGTTGCCAAGTTGGAAGCTATGTTCATTATCCTACTATCAGCACCGACATGCTCTCTGTAGTG
AAGAATCAAAATATTGGATTTAATAATGCAGCCTTCATTACCAGGAATCCTTTTCTCAGCAAAGTAAAGC
TCATCTACTACTATTTATTTGCTTTTATTTATGGACTTGTTGGTTCTTGCAGTGATGTAGTCATGGTCAA
TTCTTCTTGGACACTAAACCATATTCTCTCACTATGGAAAGTTGGGAATTGCACTAACATTGTTTATCCA
CCTTGTGATGTGCAGACATTTCTGGACATTCCCTTACATGAGAAAAAGATGACCCCAGGACATTTGCTGG
TTTCTGTTGGCCAGTTTAGGCCGGAAAAGAATCATCCATTGCAGATCAGAGCCTTTGCTAAATTGCTGAA
TAAGAAGATGGTTGAGTCACCTCCTTCGCTTAAACTTGTCCTCATTGGAGGTTGTCGTAACAAAGATGAT
GAACTTAGGGTAAACCAACTGAGAAGGCTGTCTGAGGATTTAGGAGTTCAAGAATATGTGGAATTTAAAA
TAAACATTCCATTTGATGAATTAAAGAATTATTTGTCTGAAGCAACAATTGGTCTGCATACCATGTGGAA
CGAGCATTTTGGGATTGGAGTTGTGGAGTGTATGGCAGCTGGCACAATTATCCTTGCACACAATTCGGGG
GGCCCAAAGCTTGACATTGTGATTCCTCACGAAGGAGATATAACTGGCTTTCTGGCTGAGAGTGAAGAAG
ACTATGCTGAAACTATCGCTCACATTCTTTCCATGTCTGCAGAAAAGAGACTCCAAATCAGAAAAAGTGC
TCGTGCATCTGTAAGCAGATTCTCTGATCAGGAATTTGAAGTGACATTCCTATCATCTGTGGAAAAGTTA
TTTAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC218890 representing NM_001004127
Red=Cloning site Green=Tags(s)

MAAGERSWCLCKLLRFFYSLFFPGLIVCGTLCVCLVIVLWGIRLLLQRKKKLVSTSRNGKNQMVIAFFHP
YCNAGGGGERVLWCALRALQKKYPEAVYVVYTGDVNVNGQQILEGAFRRFNIRLIHPVQFVFLRKRYLVE
DSLYPHFTLLGQSLGSIFLGWEALMQCVPDVYIDSMGYAFTLPLFKYIGGCQVGSYVHYPTISTDMLSVV
KNQNIGFNNAAFITRNPFLSKVKLIYYYLFAFIYGLVGSCSDVVMVNSSWTLNHILSLWKVGNCTNIVYP
PCDVQTFLDIPLHEKKMTPGHLLVSVGQFRPEKNHPLQIRAFAKLLNKKMVESPPSLKLVLIGGCRNKDD
ELRVNQLRRLSEDLGVQEYVEFKINIPFDELKNYLSEATIGLHTMWNEHFGIGVVECMAAGTIILAHNSG
GPKLDIVIPHEGDITGFLAESEEDYAETIAHILSMSAEKRLQIRKSARASVSRFSDQEFEVTFLSSVEKL
FK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001004127
ORF Size 1476 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001004127.1, NP_001004127.1
RefSeq Size 2585 bp
RefSeq ORF 1479 bp
Locus ID 440138
UniProt ID Q2TAA5
Cytogenetics 13q14.3
Protein Families Transmembrane
Protein Pathways Metabolic pathways, N-Glycan biosynthesis
MW 55.5 kDa
Summary This gene encodes a GDP-Man:Man3GlcNAc2-PP-dolichol-alpha1,2-mannosyltransferase which is localized to the cytosolic side of the endoplasmic reticulum (ER) and catalyzes the transfer of the fourth and fifth mannose residue from GDP-mannose (GDP-Man) to Man3GlcNAc2-PP-dolichol and Man4GlcNAc2-PP-dolichol resulting in the production of Man5GlcNAc2-PP-dolichol. Mutations in this gene are associated with congenital disorder of glycosylation type Ip (CDGIP). This gene overlaps but is distinct from the UTP14, U3 small nucleolar ribonucleoprotein, homolog C (yeast) gene. A pseudogene of the GDP-Man:Man3GlcNAc2-PP-dolichol-alpha1,2-mannosyltransferase has been identified on chromosome 19. provided by RefSeq, Aug 2010
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"NM_001004127" in other vectors (4)
SKU Description Size Price
RC218890L3 Lenti ORF clone of Human asparagine-linked glycosylation 11, alpha-1,2-mannosyltransferase homolog (yeast) (ALG11), transcript variant A, Myc-DDK-tagged 10 ug
$757.00
RC218890L4 Lenti ORF clone of Human asparagine-linked glycosylation 11, alpha-1,2-mannosyltransferase homolog (yeast) (ALG11), transcript variant A, mGFP tagged 10 ug
$757.00
RG218890 ALG11 (tGFP-tagged) - Human asparagine-linked glycosylation 11, alpha-1,2-mannosyltransferase homolog (yeast) (ALG11), transcript variant A 10 ug
$657.00
SC300606 ALG11 (untagged)-Human asparagine-linked glycosylation 11, alpha-1,2-mannosyltransferase homolog (yeast) (ALG11), transcript variant A 10 ug
$503.00

Plasmid Map for RC218890

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC218890
Circular map for RC218890

file-alt Citations

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