T (NM_003181) Human Tagged ORF Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | T |
| Synonyms | SAVA; T; TFT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003181 |
| ORF Size | 1308 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003181.4 |
| RefSeq Size | 2518 bp |
| RefSeq ORF | 1308 bp |
| Locus ID | 6862 |
| UniProt ID | O15178 |
| Cytogenetics | 6q27 |
| Protein Families | ES Cell Differentiation/IPS, Transcription Factors |
| MW | 47.3 kDa |
| Summary | The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies. provided by RefSeq, Sep 2018 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003181" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC218530L1 | Lenti ORF clone of Human T, brachyury homolog (mouse) (T), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC218530L2 | Lenti ORF clone of Human T, brachyury homolog (mouse) (T), mGFP tagged | 10 ug |
$757.00
|
|
| RC218530L3 | Lenti ORF clone of Human T, brachyury homolog (mouse) (T), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC218530L4 | Lenti ORF clone of Human T, brachyury homolog (mouse) (T), mGFP tagged | 10 ug |
$757.00
|
|
| RG218530 | T (tGFP-tagged) - Human T, brachyury homolog (mouse) (T) | 10 ug |
$489.00
$657.00
|
|
| SC303281 | T (untagged)-Human T, brachyury homolog (mouse) (T) | 10 ug |
$457.00
|
|
Citations
Transfection of T-Box Transcription Factor BRACHYURY and SOX2 Synergistically Promote Self-Renewal and Invasive Phenotype in Oral Cancer Cells
Akimoto, Nakamura, Hijioka et al
Int J Mol Sci (2018) 19 (11) DOI: 10.3390/ijms19113620
Akimoto, Nakamura, Hijioka et al
Int J Mol Sci (2018) 19 (11) DOI: 10.3390/ijms19113620
AI Summary: OriGene’s pCMV6-Brachyury vector was used to achieve forced expression of BRACHYURY in oral cancer cell lines, enabling the study of its role—alone and in combination with SOX2—in promoting self-renewal and invasive properties in these cells.
Snippet: r with an atmosphere of 5% CO2 at 37 °C. 4.2. Transfection and Knockdown of BRACHYURY and SOX2 For forced expression of BRACHYURY, full-length human Brachyury cDNA was cloned into the pCMV6 vector (ORIGENE Technologies Inc., Rockville, MD, USA), resulting in the pCMV6-Brachyury vector. ACCS-Bra and TF-Bra cells were generated via transfection of the pCMV6-Brachyury vector into ACCS-GFP and TF-GF
Loss of the Cyclin-Dependent Kinase Inhibitor 1 in the Context of Brachyury-Mediated Phenotypic Plasticity Drives Tumor Resistance to Immune Attack
Hamilton, McCampbell, Palena
Front Oncol (2018) 8, 143 DOI: 10.3389/fonc.2018.00143
Hamilton, McCampbell, Palena
Front Oncol (2018) 8, 143 DOI: 10.3389/fonc.2018.00143
AI Summary: OriGene contributed full-length human brachyury and p21CIP1 encoding fragments, which were used to generate expression constructs for functional studies in this paper.
Snippet: 19). The full-length human brachyury and p21CIP1 encoding fragments were purchased from Origene (Rockville, MD, USA) and subsequently cloned into the pcDNA3.1(+) expression vector (Thermo Fisher Scientific). Tumor Cached
Targeting Estrogen Receptor Signaling with Fulvestrant Enhances Immune and Chemotherapy-Mediated Cytotoxicity of Human Lung Cancer
Hamilton, Griner, Keller et al
Clin. Cancer Res. (2016) 22 (24), 6204-6216 DOI: 10.1158/1078-0432.CCR-15-3059
Hamilton, Griner, Keller et al
Clin. Cancer Res. (2016) 22 (24), 6204-6216 DOI: 10.1158/1078-0432.CCR-15-3059
AI Summary: OriGene products contributed to this study by providing expression vectors used for manipulating gene expression in human lung carcinoma cells to investigate the effects of targeting estrogen receptor signaling on immune and chemotherapy-mediated cytotoxicity.
Snippet: ...vectors were purchased from Origene Technologies. Brachyury and...12) using the following antibodies: pan-actin (clone Ab-5...incubated with anti-ESR1 antibody (GTX62423, GeneTex) overnight...were stained using primary antibodies against ESR1 (ab32063, Abcam...
Showing 1-3 of 3 papers.
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