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T (NM_003181) Human Untagged Clone

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SKU
SC303281
T (untagged)-Human T, brachyury homolog (mouse) (T)
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol T
Synonyms SAVA; T; TFT
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_003181 edited
TCTCAGGTAGCGAGTCTGGGCTTCGGGGACGGCGGGGAGGGGAGCCGGACGGGAGGATGA
GCTCCCCTGGCACCGAGAGCGCGGGAAAGAGCCTGCAGTACCGAGTGGACCACCTGCTGA
GCGCCGTGGAGAATGAGCTGCAGGCGGGCAGCGAGAAGGGCGACCCCACAGAGCGCGAAC
TGCGCGTGGGCCTGGAGGAGAGCGAGCTGTGGCTGCGCTTCAAGGAGCTCACCAATGAGA
TGATCGTGACCAAGAACGGCAGGAGGATGTTTCCGGTGCTGAAGGTGAACGTGTCTGGCC
TGGACCCCAACGCCATGTACTCCTTCCTGCTGGACTTCGTGGCGGCGGACAACCACCGCT
GGAAGTACGTGAACGGGGAATGGGTGCCGGGGGGCAAGCCGGAGCCGCAGGCGCCCAGCT
GCGTCTACATCCACCCCGACTCGCCCAACTTCGGGGCCCACTGGATGAAGGCTCCCGTCT
CCTTCAGCAAAGTCAAGCTCACCAACAAGCTCAACGGAGGGGGCCAGATCATGCTGAACT
CCTTGCATAAGTATGAGCCTCGAATCCACATAGTGAGAGTTGGGGGTCCACAGCGCATGA
TCACCAGCCACTGCTTCCCTGAGACCCAGTTCATAGCGGTGACTGCTTATCAGAACGAGG
AGATCACAGCTCTTAAAATTAAGTACAATCCATTTGCAAAAGCTTTCCTTGATGCAAAGG
AAAGAAGTGATCACAAAGAGATGATGGAGGAACCCGGAGACAGCCAGCAACCTGGGTACT
CCCAATCAGGGGGGTGGCTTCTTCCTGGAACCAGCACCCTGTGTCCACCTGCAAATCCTC
ATCCTCAGTTTGGAGGTGCCCTCTCCCTCCCCTCCACGCACAGCTGTGACAGGTACCCAA
CCCTGAGGAGCCACCGGTCCTCACCCTACCCCAGCCCCTATGCTCATCGGAACAATTCTC
CAACCTATTCTGACAACTCACCTGCATGTTTATCCATGCTGCAATCCCATGACAATTGGT
CCAGCCTTGGAATGCCTGCCCATCCCAGCATGCTCCCCGTGAGCCACAATGCCAGCCCAC
CTACCAGCTCCAGTCAGTACCCCAGCCTGTGGTCTGTGAGCAACGGCGCCGTCACCCCGG
GCTCCCAGGCAGCAGCCGTGTCCAACGGGCTGGGGGCCCAGTTCTTCCGGGGCTCCCCCG
CGCACTACACACCCCTCACCCATCCGGTCTCGGCGCCCTCTTCCTCGGGATCCCCACTGT
ACGAAGGGGCGGCCGCGGCCACAGACATCGTGGACAGCCAGTACGACGCCGCAGCCCAAG
GCCGCCTCATAGCCTCATGGACACCTGTGTCGCCACCTTCCATGTGAAGCAGCAAGGCCC
AGGTCCCGAAAGATGCAGTGACTTTTTGTCGTGGCAGCCAGTGGTGACTGGATTGACCTA
CTAGGTACCCAGTGGCAGTCTCAGGTTAAGAAGGAAATGCAGCCTCAGTAACTTCCTTTT
CAAAGCAGTGGAGGAGCACAC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003181 unedited
GNNCGGCCGCGNAATTCGCCCTTGNAGCAGGNAGGTGGATTCTCAGTAGCGAGTCTGGGC
TTCGGGGACGGCGGGGAGGGGAGCCGGACGGGAGGATGAGCTCCCCTGGCACCGAGAGCG
CGGGAAAGAGCCTGCAGTACCGAGTGGACCACCTGCTGAGCGCCGTGGAGAATGAGCTGC
AGGCGGGCAGCGAGAAGGGCGACCCCACAGAGCGCGAACTGCGCGTGGGCCTGGAGGAGA
GCGAGCTGTGGCTGCGCTTCAAGGAGCTCACCAATGAGATGATCGTGACCAAGAACGGCA
GGAGGATGTTTCCGGTGCTGAAGGTGAACGTGTCTGGCCTGGACCCCAACGCCATGTACT
CCTTCCTGCTGGACTTCGTGGCGGCGGACAACCACCGCTGGAAGTACGTGAACGGNGAAT
GGGTGCCGGGGGGCAAGCCGGAGCCGCAGGCGCCCAGCTGCGTCTACATCCACCCCGACT
CGCCCAACTTCGGGGCCCACTGGATGAAGGCTCCCGTCTCCTTCAGCANAGTCAAGCTCA
CCAACNAGCTCAACGGGAGGNGGCCAGATCATGCTGAACTCCTTTGCATAGTATGAGCCT
CGAATCCACATAGTGAGAGTTGGGGGGTCCACAGCGCATGATCACCAGCCACTGCTTCCC
TGAGACCCAGTTCATAGCGGTGACTGCTTATCAGAACGAGGAGATCACAGCTCTTAAAAT
TAAGTANCATCCATTTGCAAAAGCTTTCCTTGATGCANAGGANAGAAGTGATCACAAAGA
GATGAATGGANGACCCGNAGACAGCCAGCAACCTGGGTACTCCCATCA
3' Read Nucleotide Sequence
>Reverse primer walk for NM_003181 unedited
GACTTCTGGATAGTCAATCCATTCCCACTGGCTGCCACGAAAAAATCACTGCATCTTTCG
GGACCTGGGCCTTGCTGCTTCACATGGAAGGTGGCGACACAGGTGTCCATGAGGCTATGA
GGCGGCCTTGGGCTGCGGCGTCGTACTGGCTGTCCACGATGTCTGTGGCCGCGGCCGCCC
CTTCGTACAGTGGGGATCCCGAGGAAGAGGGCGCCGAGACCGGATGGGTGAGGGGTGTGT
AGTGCGCGGGGGAGCCCCGGAAGAACTGGGCCCCCAGCCCGTTGGACACGGCTGCTGCCT
GGGAGCCCGGGGTGACGGCGCCGTTGCTCACAGACCACAGGCTGGGGTACTGACTGGAGC
TGGTAGGTGGGCTGGCATTGTGGCTCACGGGGAGCATGCTGGGATGGGCAGGCATTCCAA
GGCTGGACCAATTGTCATGGGATTGCAGCATGGATAAACATGCAGGTGAGTTGTCAGAAT
AGGTTGGAGAATTGTTCCGATGAGCATAGGGGCTGGGGTAGGGTGAGGACCGGTGGCTCC
TCAGGGTTGGGTACCTGTCACAGCTGTGCGTGGAGGGGAGGGAGAGGGCACCTCCAAACT
GAGGATGAGGATTTGCAGGTGGACACAGGGTGCTGGTTCCAGGAAGAAGCCACCCCCCTG
ATTGGGAGTACCCAGGTTGCTGGCTGTCTCCGGGTTCCTCCATCATCTCTTTGTGATCAC
TTCTTTCCTTTGCATCAAGGAAAGCTTTTGCAAATGGATTGTACTTAATTTTAAGAGCTG
TGATCTCCTCGTTCTGATAAGCAGTCACCGCTATGAACTGGGTCTCAGGGAAGCAGTGGC
TA
Restriction Sites Please inquire |
ACCN NM_003181
Insert Size 1500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation There is 4 nucleotide difference between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the insertion of 1aa and substituion of 1 aa.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003181.2, NP_003172.1
RefSeq Size 2518 bp
RefSeq ORF 1308 bp
Locus ID 6862
UniProt ID O15178
Cytogenetics 6q27
Protein Families ES Cell Differentiation/IPS, Transcription Factors
Summary The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies. provided by RefSeq, Sep 2018
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_003181" in other vectors (6)
SKU Description Size Price
RC218530 T (Myc-DDK-tagged)-Human T, brachyury homolog (mouse) (T) 10 ug
$457.00
RC218530L1 Lenti ORF clone of Human T, brachyury homolog (mouse) (T), Myc-DDK-tagged 10 ug
$757.00
RC218530L2 Lenti ORF clone of Human T, brachyury homolog (mouse) (T), mGFP tagged 10 ug
$757.00
RC218530L3 Lenti ORF clone of Human T, brachyury homolog (mouse) (T), Myc-DDK-tagged 10 ug
$757.00
RC218530L4 Lenti ORF clone of Human T, brachyury homolog (mouse) (T), mGFP tagged 10 ug
$757.00
RG218530 T (tGFP-tagged) - Human T, brachyury homolog (mouse) (T) 10 ug
$489.00 $657.00

file-alt Citations

Nonviral Transfection With Brachyury Reprograms Human Intervertebral Disc Cells to a Pro-Anabolic Anti-Catabolic/Inflammatory Phenotype: A Proof of Concept Study
Tang, Richards, Khan et al
J. Orthop. Res. (2019)
AI Summary: In this study, OriGene's pCMV6-XL5 vector containing the Brachyury gene (Cat: SC303281) was used for nonviral transfection to reprogram human intervertebral disc cells, enabling the investigation of cellular phenotype changes relevant to disc degeneration therapy.
Snippet: T, with pCMV6-XL5 vector and antibiotic selection marker, ampicillin, was obtained from OriGene Technologies (Reference sequence from NCBI-NIH: NM_003181, Cat: SC303281;Supplemental Table 1). The plasmids were transformed
Showing 1-1 of 1 papers.
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