Protor 1 (PRR5) (NM_015366) Human Tagged ORF Clone

SKU RC217823

PRR5 (Myc-DDK-tagged)-Human proline rich 5 (renal) (PRR5), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Protor 1
• Synonyms: FLJ20185k; PP610; PROTOR-1; PROTOR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217823 representing NM_015366; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGTTCGCCCAGCCTCAGTGACCTGGGCAAGAGAGAGCCGGCCGCCGCCGCGGACGAGCGGGGCACGC; AGCAGCGCCGGGCCTGCGCCAACGCCACCTGGAACAGCATCCACAACGGGGTGATCGCCGTCTTCCAGCG; CAAGGGGCTGCCCGACCAGGAGCTCTTCAGCCTCAACGAGGGCGTCCGGCAGCTGTTGAAGACAGAGCTG; GGGTCCTTCTTCACGGAGTACCTGCAGAACCAGCTGCTGACAAAAGGCATGGTGATCCTTCGGGACAAGA; TTCGCTTCTATGAGGGACAGAAGCTGCTGGACTCACTGGCAGAGACCTGGGACTTCTTCTTCAGTGACGT; GCTGCCCATGCTGCAGGCCATCTTCTACCCGGTGCAGGGCAAGGAGCCATCGGTGCGCCAGCTGGCCCTG; CTGCACTTCCGGAATGCCATCACCCTCAGTGTGAAGCTAGAGGATGCGCTGGCCCGGGCCCATGCCCGTG; TGCCCCCTGCCATCGTGCAGATGCTGCTGGTGCTGCAGGGGGTACATGAGTCCAGGGGCGTGACTGAGGA; CTACCTGCGCCTGGAGACGCTGGTCCAGAAGGTGGTGTCGCCATACCTGGGCACCTACGGCCTCCACTCC; AGCGAGGGGCCCTTCACCCATTCCTGCATCCTGGAAAAGCGCCTCCTCCGCCGCTCCCGCTCGGGGGACG; TGCTGGCCAAGAACCCTGTGGTGCGCTCCAAGAGCTACAACACGCCTCTGCTGAACCCCGTGCAGGAGCA; CGAGGCGGAGGGCGCGGCGGCCGGCGGTACCAGCATCCGCAGGCACTCTGTGTCGGAGATGACGTCCTGC; CCCGAGCCTCAGGGCTTCTCCGACCCGCCCGGCCAGGGCCCCACCGGGACCTTCAGGTCCTCCCCGGCGC; CCCACTCAGGGCCCTGCCCCAGCAGACTGTACCCCACGACCCAGCCCCCTGAGCAGGGCTTGGATCCCAC; CCGCAGCTCCCTGCCCCGCTCCAGCCCGGAGAACCTGGTGGACCAGATCCTGGAGTCCGTGGACTCGGAT; TCTGAAGGGATTTTCATTGACTTTGGCCGGGGCCGGGGCTCTGGCATGTCCGACTTGGAGGGCTCTGGGG; GCCGGCAGAGTGTCGTG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217823 representing NM_015366; Red=Cloning site Green=Tags(s); ; MSSPSLSDLGKREPAAAADERGTQQRRACANATWNSIHNGVIAVFQRKGLPDQELFSLNEGVRQLLKTEL; GSFFTEYLQNQLLTKGMVILRDKIRFYEGQKLLDSLAETWDFFFSDVLPMLQAIFYPVQGKEPSVRQLAL; LHFRNAITLSVKLEDALARAHARVPPAIVQMLLVLQGVHESRGVTEDYLRLETLVQKVVSPYLGTYGLHS; SEGPFTHSCILEKRLLRRSRSGDVLAKNPVVRSKSYNTPLLNPVQEHEAEGAAAGGTSIRRHSVSEMTSC; PEPQGFSDPPGQGPTGTFRSSPAPHSGPCPSRLYPTTQPPEQGLDPTRSSLPRSSPENLVDQILESVDSD; SEGIFIDFGRGRGSGMSDLEGSGGRQSVV; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015366
• ORF Size: 1137 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015366.4
• RefSeq Size: 1653 bp
• RefSeq ORF: 1140 bp
• Locus ID: 55615
• UniProt ID: P85299
• Cytogenetics: 22q13.31
• Domains: RhoGAP, SEC14
• MW: 41.4 kDa
• Summary: This gene encodes a protein with a proline-rich domain. This gene is located in a region of chromosome 22 reported to contain a tumor suppressor gene that may be involved in breast and colorectal tumorigenesis. The protein is a component of the mammalian target of rapamycin complex 2 (mTORC2), and it regulates platelet-derived growth factor (PDGF) receptor beta expression and PDGF signaling to Akt and S6K1. Alternative splicing and the use of alternative promoters results in transcripts encoding different isoforms. Read-through transcripts from this gene into the downstream Rho GTPase activating protein 8 (ARHGAP8) gene also exist, which led to the original description of PRR5 and ARHGAP8 being a single gene. [provided by RefSeq, Nov 2010]