OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

VPS16 (NM_080413) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC217614
VPS16 (Myc-DDK-tagged)-Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $636.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA307915 Rabbit polyclonal antibody to VPS16 (vacuolar protein sorting 16 homolog (S. cerevisiae))
100 ul
$665.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "VPS16 or NM_080413"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol VPS16
Synonyms DYT30; hVPS16
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217614 representing NM_080413
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTGCTACACGGCGAACTGGAACCCACTCGGGGACTCTGCCTTTTACCGGAAATATGAGCTGTACA
GCATGGACTGGGACCTGAAGGAGGAACTCAGGGATTGCCTGGTGGCTGCTGCACCCTATGGGGGCCCCAT
TGCACTGCTGAGGAACCCCTGGCGGAAGGAGAAAGCTGCTAGTGTGAGGCCAGTGCTCGATATATACTCT
GCTTCCGGCATGCCTCTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGCTGGTCAGCTG
AGGAGGAGCTGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGGCTTCATGGTGACTTCCGGAG
ACACTTCAGCATGGGCAATGAAGTGCTCCAGAACCGGGTTCTGGATGCCCGGATCTTTCACACTGAGTTT
GGTTCCGGAGTGGCCATCCTCACAGGGGCCCACCGCTTCACCCTCAGTGCCAATGTGGGTGACCTCAAAC
TCCGCCGGATGCCAGAGGTGCCAGGTCTGCAAAGTGCACCCTCCTGCTGGACTGTGCTGTGCCAGGACCG
AGTGGCACACATTCTTCTGGCTGTGGGGCCTGACCTTTACCTCTTGGACCATGCAGCCTGCTCCGCAGTG
ACGCCCCCTGGCCTGGCCCCAGGAGTAAGCAGCTTCCTACAGATGGCTGTCTCCTTCACCTACCGACACC
TGGCACTCTTCACAGACACAGGCTACATCTGGATGGGGACAGCATCACTCAAGGAGAAGCTATGTGAGTT
CAACTGCAACATCCGGGCACCTCCAAAGCAGATGGTCTGGTGCAGCCGTCCTCGTAGCAAGGAGAGGGCC
GTGGTGGTGGCCTGGGAAAGGCGGCTGATGGTGGTGGGCGATGCACCCGAGAGCATCCAATATAAGCAGC
TCACCATCCAGGTGCTGCTGGACAGGCTCGTGTTGCGGAGACTTTACCCCCTGGCCATCCAGATATGCGA
GTACTTGCGCCTTCCTGAAGTACAGGGCGTCAGCAGGATCCTGGCCCACTGGGCCTGCTACAAGGTGCAA
CAGAAGGATGTCTCAGATGAGGATGTGGCTCGAGCCATTAACCAGAAGCTGGGGGACACGCCTGGTGTCT
CTTACTCCGACATTGCTGCACGAGCCTATGGTTGTGGCCGCACGGAGCTGGCCATCAAGCTGCTGGAGTA
TGAGCCACGCTCAGGGGAGCAGGTACCCCTTCTCCTAAAGATGAAGAGGAGCAAACTGGCACTAAGCAAG
GCCATCGAGAGCGGGGACACTGACCTGGTGTTCACGGTGTTGCTGCACCTGAAGAACGAGCTGAACCGAG
GAGATTTTTTCATGACCCTTCGGAATCAGCCCATGGCCCTCAGTTTGTACCGACAGTTCTGTAAGCATCA
GGAGCTAGAGACGCTGAAGGACCTTTACAATCAGGATGACAATCACCAGGAATTGGGCAGCTTCCACATC
CGAGCCAGCTATGCTGCAGAAGAGCGTATTGAGGGGCGAGTAGCAGCTCTGCAGACAGCCGCCGATGCCT
TCTACAAGGCCAAGAATGAGTTTGCAGCCAAGGCTACAGAGGATCAAATGCGGCTCCTACGGCTGCAGCG
GCGCCTAGAAGACGAGCTGGGGGGCCAGTTCCTAGACCTGTCTCTACATGACACAGTTACCACCCTCATT
CTTGGCGGTCACAACAAGCGTGCAGAGCAGCTGGCACGTGACTTCCGCATCCCTGACAAGAGGCTCTGGT
GGCTGAAGCTGACTGCCCTGGCAGATTTGGAAGATTGGGAAGAGCTAGAGAAGTTTTCCAAGAGCAAGAA
ATCACCCATTGGCTACCTGCCTTTTGTGGAGATCTGCATGAAACAACATAACAAATACGAAGCCAAGAAG
TATGCTTCCCGCGTGGGTCCCGAGCAGAAGGTCAAGGCTTTGCTTCTTGTTGGCGATGTGGCTCAGGCTG
CAGATGTGGCCATCGAACACCGGAATGAGGCTGAGCTGAGCCTCGTATTGTCCCACTGCACGGGAGCCAC
AGATGGGGCCACAGCTGACAAGATTCAACGGGCCAGGGCACAAGCCCAGAAGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC217614 representing NM_080413
Red=Cloning site Green=Tags(s)

MDCYTANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWRKEKAASVRPVLDIYS
ASGMPLASLLWKSGPVVSLGWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEF
GSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLQSAPSCWTVLCQDRVAHILLAVGPDLYLLDHAACSAV
TPPGLAPGVSSFLQMAVSFTYRHLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERA
VVVAWERRLMVVGDAPESIQYKQLTIQVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWACYKVQ
QKDVSDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSK
AIESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELETLKDLYNQDDNHQELGSFHI
RASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRLQRRLEDELGGQFLDLSLHDTVTTLI
LGGHNKRAEQLARDFRIPDKRLWWLKLTALADLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKYEAKK
YASRVGPEQKVKALLLVGDVAQAADVAIEHRNEAELSLVLSHCTGATDGATADKIQRARAQAQKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080413
ORF Size 2085 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080413.2, NP_536338.1
RefSeq Size 2337 bp
RefSeq ORF 2088 bp
Locus ID 64601
UniProt ID Q9H269
Cytogenetics 20p13
Domains Vps16_C, Vps16_N
Protein Families Druggable Genome
MW 78.1 kDa
Summary Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps16 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2009
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_080413" in other vectors (4)
SKU Description Size Price
RC217614L3 Lenti-ORF clone of VPS16 (Myc-DDK-tagged)-Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 3 10 ug
$936.00
RC217614L4 Lenti-ORF clone of VPS16 (mGFP-tagged)-Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 3 10 ug
$936.00
RG217614 VPS16 (tGFP-tagged) - Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 3 10 ug
$836.00
SC314454 VPS16 (untagged)-Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 3 10 ug
$701.00

Plasmid Map for RC217614

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC217614
Circular map for RC217614

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products