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VPS16 (NM_080413) Human Untagged Clone

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SKU
SC314454
VPS16 (untagged)-Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 3
  $701.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol VPS16
Synonyms DYT30; hVPS16
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC314454 representing NM_080413.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGACTGCTACACGGCGAACTGGAACCCACTCGGGGACTCTGCCTTTTACCGGAAATATGAGCTGTAC
AGCATGGACTGGGACCTGAAGGAGGAACTCAGGGATTGCCTGGTGGCTGCTGCACCCTATGGGGGCCCC
ATTGCACTGCTGAGGAACCCCTGGCGGAAGGAGAAAGCTGCTAGTGTGAGGCCAGTGCTCGATATATAC
TCTGCTTCCGGCATGCCTCTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGCTGGTCA
GCTGAGGAGGAGCTGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGGCTTCATGGTGACTTC
CGGAGACACTTCAGCATGGGCAATGAAGTGCTCCAGAACCGGGTTCTGGATGCCCGGATCTTTCACACT
GAGTTTGGTTCCGGAGTGGCCATCCTCACAGGGGCCCACCGCTTCACCCTCAGTGCCAATGTGGGTGAC
CTCAAACTCCGCCGGATGCCAGAGGTGCCAGGTCTGCAAAGTGCACCCTCCTGCTGGACTGTGCTGTGC
CAGGACCGAGTGGCACACATTCTTCTGGCTGTGGGGCCTGACCTTTACCTCTTGGACCATGCAGCCTGC
TCCGCAGTGACGCCCCCTGGCCTGGCCCCAGGAGTAAGCAGCTTCCTACAGATGGCTGTCTCCTTCACC
TACCGACACCTGGCACTCTTCACAGACACAGGCTACATCTGGATGGGGACAGCATCACTCAAGGAGAAG
CTATGTGAGTTCAACTGCAACATCCGGGCACCTCCAAAGCAGATGGTCTGGTGCAGCCGTCCTCGTAGC
AAGGAGAGGGCCGTGGTGGTGGCCTGGGAAAGGCGGCTGATGGTGGTGGGCGATGCACCCGAGAGCATC
CAATATAAGCAGCTCACCATCCAGGTGCTGCTGGACAGGCTCGTGTTGCGGAGACTTTACCCCCTGGCC
ATCCAGATATGCGAGTACTTGCGCCTTCCTGAAGTACAGGGCGTCAGCAGGATCCTGGCCCACTGGGCC
TGCTACAAGGTGCAACAGAAGGATGTCTCAGATGAGGATGTGGCTCGAGCCATTAACCAGAAGCTGGGG
GACACGCCTGGTGTCTCTTACTCCGACATTGCTGCACGAGCCTATGGTTGTGGCCGCACGGAGCTGGCC
ATCAAGCTGCTGGAGTATGAGCCACGCTCAGGGGAGCAGGTACCCCTTCTCCTAAAGATGAAGAGGAGC
AAACTGGCACTAAGCAAGGCCATCGAGAGCGGGGACACTGACCTGGTGTTCACGGTGTTGCTGCACCTG
AAGAACGAGCTGAACCGAGGAGATTTTTTCATGACCCTTCGGAATCAGCCCATGGCCCTCAGTTTGTAC
CGACAGTTCTGTAAGCATCAGGAGCTAGAGACGCTGAAGGACCTTTACAATCAGGATGACAATCACCAG
GAATTGGGCAGCTTCCACATCCGAGCCAGCTATGCTGCAGAAGAGCGTATTGAGGGGCGAGTAGCAGCT
CTGCAGACAGCCGCCGATGCCTTCTACAAGGCCAAGAATGAGTTTGCAGCCAAGGCTACAGAGGATCAA
ATGCGGCTCCTACGGCTGCAGCGGCGCCTAGAAGACGAGCTGGGGGGCCAGTTCCTAGACCTGTCTCTA
CATGACACAGTTACCACCCTCATTCTTGGCGGTCACAACAAGCGTGCAGAGCAGCTGGCACGTGACTTC
CGCATCCCTGACAAGAGGCTCTGGTGGCTGAAGCTGACTGCCCTGGCAGATTTGGAAGATTGGGAAGAG
CTAGAGAAGTTTTCCAAGAGCAAGAAATCACCCATTGGCTACCTGCCTTTTGTGGAGATCTGCATGAAA
CAACATAACAAATACGAAGCCAAGAAGTATGCTTCCCGCGTGGGTCCCGAGCAGAAGGTCAAGGCTTTG
CTTCTTGTTGGCGATGTGGCTCAGGCTGCAGATGTGGCCATCGAACACCGGAATGAGGCTGAGCTGAGC
CTCGTATTGTCCCACTGCACGGGAGCCACAGATGGGGCCACAGCTGACAAGATTCAACGGGCCAGGGCA
CAAGCCCAGAAGAAGTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_080413
Insert Size 2088 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080413.2
RefSeq Size 2341 bp
RefSeq ORF 2088 bp
Locus ID 64601
UniProt ID Q9H269
Cytogenetics 20p13
Domains Vps16_C, Vps16_N
Protein Families Druggable Genome
MW 78.3 kDa
Summary Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps16 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2009
Transcript Variant: This variant (3) lacks an internal sequence segment compared to transcript variant 1. It thus encodes a shorter isoform (3) which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_080413" in other vectors (4)
SKU Description Size Price
RC217614 VPS16 (Myc-DDK-tagged)-Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 3 10 ug
$636.00
RC217614L3 Lenti-ORF clone of VPS16 (Myc-DDK-tagged)-Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 3 10 ug
$936.00
RC217614L4 Lenti-ORF clone of VPS16 (mGFP-tagged)-Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 3 10 ug
$936.00
RG217614 VPS16 (tGFP-tagged) - Human vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16), transcript variant 3 10 ug
$836.00

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