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RPGRIP1 (NM_020366) Human Tagged ORF Clone

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SKU
RC217420
RPGRIP1 (Myc-DDK-tagged)-Human retinitis pigmentosa GTPase regulator interacting protein 1 (RPGRIP1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,119.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RPGRIP1
Synonyms CORD13; LCA6; RGI1; RGRIP; RPGRIP; RPGRIP1d
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217420 representing NM_020366
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCACATCTGGTGGACCCTACATCAGGAGACTTGCCAGTTAGAGACATAGATGCTATACCTCTGGTGC
TACCAGCCTCAAAAGGTAAGAATATGAAAACTCAACCACCCTTGAGCAGGATGAACCGGGAGGAATTGGA
GGACAGTTTCTTTCGACTTCGCGAAGATCACATGTTGGTGAAGGAGCTTTCTTGGAAGCAACAGGATGAG
ATCAAAAGGCTGAGGACCACCTTGCTGCGGTTGACCGCTGCTGGCCGGGACCTGCGGGTCGCGGAGGAGG
CGGCGCCGCTCTCGGAGACCGCAAGGCGCGGGCAGAAGGCGGGATGGCGGCAGCGCCTCTCCATGCACCA
GCGCCCCCAGATGCACCGACTGCAAGGGCATTTCCACTGCGTCGGCCCTGCCAGCCCCCGCCGCGCCCAG
CCTCGCGTCCAAGTGGGACACAGACAGCTCCACACAGCCGGTGCACCGGTGCCGGAGAAACCCAAGAGGG
GGCCAAGGGACAGGCTGAGCTACACAGCCCCTCCATCGTTTAAGGAGCATGCGACAAATGAAAACAGAGG
TGAAGTAGCCAGTAAACCCAGTGAACTTGTTTCTGGTTCTAACAGCATAATTTCTTTCAGCAGTGTCATA
AGTATGGCTAAACCCATTGGTCTATGCATGCCTAACAGTGCCCACATCATGGCCAGCAATACCATGCAAG
TGGAAGAGCCACCCAAGTCTCCTGAGAAAATGTGGCCTAAAGATGAAAATTTTGAACAGAGAAGCTCATT
GGAGTGTGCTCAGAAGGCTGCAGAGCTTCGAGCTTCCATTAAAGAGAAGGTAGAGCTGATTCGACTTAAG
AAGCTCTTACATGAAAGAAATGCTTCATTGGTTATGACAAAAGCACAATTAACAGAAGTTCAAGAGGCAT
ACGAAACCTTGCTCCAGAAGAATCAGGGAATCCTGAGTGCAGCCCATGAGGCCCTCCTCAAGCAAGTGAA
TGAGCTCAGGGCAGAGCTGAAGGAAGAAAGCAAGAAGGCTGTGAGCTTGAAGAGCCAACTGGAAGATGTG
TCTATCTTGCAGATGACTCTGAAGGAGTTTCAGGAGAGAGTTGAAGATTTGGAAAAAGAACGAAAATTGC
TGAATGACAATTATGACAAACTCTTAGAAAGCATGCTGGACAGCAGTGACAGCTCCAGTCAGCCCCACTG
GAGCAACGAGCTCATAGCGGAACAGCTACAGCAGCAAGTCTCTCAGCTGCAGGATCAGCTGGATGCTGAG
CTGGAGGACAAGAGAAAAGTTTTACTTGAGCTGTCCAGGGAGAAAGCCCAAAATGAGGATCTGAAGCTTG
AAGTCACCAACATACTTCAGAAGCATAAACAGGAAGTAGAGCTCCTCCAAAATGCAGCCACAATTTCCCA
ACCTCCTGACAGGCAATCTGAACCAGCCACTCACCCAGCTGTATTGCAAGAGAACACTCAGATCGAGCCA
AGTGAACCCAAAAACCAAGAAGAAAAGAAACTGTCCCAGGTGCTAAATGAGTTGCAAGTATCACACGCAG
AGACCACATTGGAACTAGAAAAGACCAGGGACATGCTTATTCTGCAGCGCAAAATCAACGTGTGTTATCA
GGAGGAACTGGAGGCAATGATGACAAAAGCTGACAATGATAATAGAGATCACAAAGAAAAGCTGGAGAGG
TTGACTCGACTACTAGACCTCAAGAATAACCGTATCAAGCAGCTGGAAGGTATTTTAAGAAGCCATGACC
TTCCAACATCTGAACAGCTCAAAGATGTTGCTTATGGCACCCGACCGTTGTCGTTATGTTTGGAAACACT
GCCAGCCCATGGAGATGAGGATAAAGTGGATATTTCTCTGCTGCATCAGGGTGAGAATCTTTTTGAACTG
CACATCCACCAGGCCTTCCTGACATCTGCCGCCCTAGCTCAGGCTGGAGATACCCAACCTACCACTTTCT
GCACCTATTCCTTCTATGACTTTGAAACCCACTGTACCCCATTATCTGTGGGGCCACAGCCCCTCTATGA
CTTCACCTCCCAGTATGTGATGGAGACAGATTCGCTTTTCTTACACTACCTTCAAGAGGCTTCAGCCCGG
CTTGACATACACCAGGCCATGGCCAGTGAACACAGCACTCTTGCTGCAGGATGGATTTGCTTTGACAGGG
TGCTAGAGACTGTGGAGAAAGTCCATGGCTTGGCCACACTGATTGGAGCTGGTGGAGAAGAGTTCGGGGT
TCTAGAGTACTGGATGAGGCTGCGTTTCCCCATAAAACCCAGCCTACAGGCGTGCAATAAACGAAAGAAA
GCCCAGGTCTACCTGTCAACCGATGTGCTTGGAGGCCGGAAGGCCCAGGAAGAGGAGTTCAGATCGGAGT
CTTGGGAACCTCAGAACGAGCTGTGGATTGAAATCACCAAGTGCTGTGGCCTCCGGAGTCGATGGCTGGG
AACTCAACCCAGTCCATATGCTGTGTACCGCTTCTTCACCTTTTCTGACCATGACACTGCCATCATTCCA
GCCAGTAACAACCCCTACTTTAGAGACCAGGCTCGATTCCCAGTGCTTGTGACCTCTGACCTGGACCATT
ATCTGAGACGGGAGGCCTTGTCTATACATGTTTTTGATGATGAAGACTTAGAGCCTGGCTCGTATCTTGG
CCGAGCCCGAGTGCCTTTACTGCCTCTTGCAAAAAATGAATCTATCAAAGGTGATTTTAACCTCACTGAC
CCTGCAGAGAAACCCAACGGATCTATTCAAGTGCAACTGGATTGGAAGTTTCCCTACATACCCCCTGAGA
GCTTCCTGAAACCAGAAGCTCAGACTAAGGGGAAGGATACCAAGGACAGTTCAAAGATCTCATCTGAAGA
GGAAAAGGCTTCATTTCCTTCCCAGGATCAGATGGCATCTCCTGAGGTTCCCATTGAAGCTGGCCAGTAT
CGATCTAAGAGAAAACCTCCTCATGGGGGAGAAAGAAAGGAGAAGGAGCACCAGGTTGTGAGCTACTCAA
GAAGAAAACATGGCAAAAGAATAGGTGTTCAAGGAAAGAATAGAATGGAGTATCTTAGCCTTAACATCTT
AAATGGAAATACACCACAGCAGGTGAATTACACTGAGTGGAAGTTCTCAGAGACTAACAGCTTCATAGGT
GATGGCTTTAAAAATCAGCACGAGGAAGAGGAAATGACATTATCCCATTCAGCACTGAAACAGAAGGAAC
CTCTACATCCTGTAAATGACAAAGAATCCTCTGAACAAGGTTCTGAAGTCAGTGAAGCACAAACTACCGA
CAGTGATGATGTCATAGTGCCACCCATGTCTCAGAAATATCCTAAGGCAGATTCAGAGAAGATGTGCATT
GAAATTGTCTCCCTGGCCTTCTACCCAGAGGCAGAAGTGATGTCTGATGAGAACATAAAACAGGTGTATG
TGGAGTACAAATTCTACGACCTACCCTTGTCGGAGACAGAGACTCCAGTGTCCCTAAGGAAGCCTAGGGC
AGGAGAAGAAATCCACTTTCACTTTAGCAAGGTAATAGACCTGGACCCACAGGAGCAGCAAGGCCGAAGG
CGGTTTCTGTTCGACATGCTGAATGGACAAGATCCTGATCAAGGACATTTAAAGTTTACAGTGGTAAGTG
ATCCTCTGGATGAAGAAAAGAAAGAATGTGAAGAAGTGGGATATGCATATCTTCAACTGTGGCAGATCCT
GGAGTCAGGAAGAGATATTCTAGAGCAAGAGCTAGACATTGTTAGCCCTGAAGATCTGGCTACCCCAATA
GGAAGGCTGAAGGTTTCCCTTCAAGCAGCTGCTGTCCTCCATGCTATTTACAAGGAGATGACTGAAGATT
TGTTTTCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC217420 representing NM_020366
Red=Cloning site Green=Tags(s)

MSHLVDPTSGDLPVRDIDAIPLVLPASKGKNMKTQPPLSRMNREELEDSFFRLREDHMLVKELSWKQQDE
IKRLRTTLLRLTAAGRDLRVAEEAAPLSETARRGQKAGWRQRLSMHQRPQMHRLQGHFHCVGPASPRRAQ
PRVQVGHRQLHTAGAPVPEKPKRGPRDRLSYTAPPSFKEHATNENRGEVASKPSELVSGSNSIISFSSVI
SMAKPIGLCMPNSAHIMASNTMQVEEPPKSPEKMWPKDENFEQRSSLECAQKAAELRASIKEKVELIRLK
KLLHERNASLVMTKAQLTEVQEAYETLLQKNQGILSAAHEALLKQVNELRAELKEESKKAVSLKSQLEDV
SILQMTLKEFQERVEDLEKERKLLNDNYDKLLESMLDSSDSSSQPHWSNELIAEQLQQQVSQLQDQLDAE
LEDKRKVLLELSREKAQNEDLKLEVTNILQKHKQEVELLQNAATISQPPDRQSEPATHPAVLQENTQIEP
SEPKNQEEKKLSQVLNELQVSHAETTLELEKTRDMLILQRKINVCYQEELEAMMTKADNDNRDHKEKLER
LTRLLDLKNNRIKQLEGILRSHDLPTSEQLKDVAYGTRPLSLCLETLPAHGDEDKVDISLLHQGENLFEL
HIHQAFLTSAALAQAGDTQPTTFCTYSFYDFETHCTPLSVGPQPLYDFTSQYVMETDSLFLHYLQEASAR
LDIHQAMASEHSTLAAGWICFDRVLETVEKVHGLATLIGAGGEEFGVLEYWMRLRFPIKPSLQACNKRKK
AQVYLSTDVLGGRKAQEEEFRSESWEPQNELWIEITKCCGLRSRWLGTQPSPYAVYRFFTFSDHDTAIIP
ASNNPYFRDQARFPVLVTSDLDHYLRREALSIHVFDDEDLEPGSYLGRARVPLLPLAKNESIKGDFNLTD
PAEKPNGSIQVQLDWKFPYIPPESFLKPEAQTKGKDTKDSSKISSEEEKASFPSQDQMASPEVPIEAGQY
RSKRKPPHGGERKEKEHQVVSYSRRKHGKRIGVQGKNRMEYLSLNILNGNTPQQVNYTEWKFSETNSFIG
DGFKNQHEEEEMTLSHSALKQKEPLHPVNDKESSEQGSEVSEAQTTDSDDVIVPPMSQKYPKADSEKMCI
EIVSLAFYPEAEVMSDENIKQVYVEYKFYDLPLSETETPVSLRKPRAGEEIHFHFSKVIDLDPQEQQGRR
RFLFDMLNGQDPDQGHLKFTVVSDPLDEEKKECEEVGYAYLQLWQILESGRDILEQELDIVSPEDLATPI
GRLKVSLQAAAVLHAIYKEMTEDLFS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020366
ORF Size 3858 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020366.2, NP_065099.2
RefSeq Size 3861 bp
RefSeq ORF 3861 bp
Locus ID 57096
UniProt ID Q96KN7
Cytogenetics 14q11.2
Protein Families Druggable Genome
MW 146.5 kDa
Summary This gene encodes a photoreceptor protein that interacts with retinitis pigmentosa GTPase regulator protein and is a key component of cone and rod photoreceptor cells. Mutations in this gene lead to autosomal recessive congenital blindness. provided by RefSeq, Oct 2008
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"NM_020366" in other vectors (6)
SKU Description Size Price
RC217420L1 Lenti ORF clone of Human retinitis pigmentosa GTPase regulator interacting protein 1 (RPGRIP1), Myc-DDK-tagged 10 ug
$1,419.00
RC217420L2 Lenti ORF clone of Human retinitis pigmentosa GTPase regulator interacting protein 1 (RPGRIP1), mGFP tagged 10 ug
$1,419.00
RC217420L3 Lenti ORF clone of Human retinitis pigmentosa GTPase regulator interacting protein 1 (RPGRIP1), Myc-DDK-tagged 10 ug
$1,419.00
RC217420L4 Lenti ORF clone of Human retinitis pigmentosa GTPase regulator interacting protein 1 (RPGRIP1), mGFP tagged 10 ug
$1,419.00
RG217420 RPGRIP1 (tGFP-tagged) - Human retinitis pigmentosa GTPase regulator interacting protein 1 (RPGRIP1) 10 ug
$789.00 $1,319.00
SC304750 RPGRIP1 (untagged)-Human retinitis pigmentosa GTPase regulator interacting protein 1 (RPGRIP1) 10 ug
$1,120.00

Plasmid Map for RC217420

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC217420
Circular map for RC217420

file-alt Citations

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