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RPGRIP1 (NM_020366) Human Untagged Clone

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SKU
SC304750
RPGRIP1 (untagged)-Human retinitis pigmentosa GTPase regulator interacting protein 1 (RPGRIP1)
  $1,120.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol RPGRIP1
Synonyms CORD13; LCA6; RGI1; RGRIP; RPGRIP; RPGRIP1d
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_020366 edited
ATGTCACATCTGGTGGACCCTACATCAGGAGACTTGCCAGTTAGAGACATAGATGCTATA
CCTCTGGTGCTACCAGCCTCAAAAGGTAAGAATATGAAAACTCAACCACCCTTGAGCAGG
ATGAACCGGGAGGAATTGGAGGACAGTTTCTTTCGACTTCGCGAAGATCACATGTTGGTG
AAGGAGCTTTCTTGGAAGCAACAGGATGAGATCAAAAGGCTGAGGACCACCTTGCTGCGG
TTGACCGCTGCTGGCCGGGACCTGCGGGTCGCGGAGGAGGCGGCGCCGCTCTCGGAGACC
GCAAGGCGCGGGCAGAAGGCGGGATGGCGGCAGCGCCTCTCCATGCACCAGCGCCCCCAG
ATGCACCGACTGCAAGGGCATTTCCACTGCGTCGGCCCTGCCAGCCCCCGCCGCGCCCAG
CCTCGCGTCCAAGTGGGACACAGACAGCTCCACACAGCCGGTGCACCGGTGCCGGAGAAA
CCCAAGAGGGGGCCAAGGGACAGGCTGAGCTACACAGCCCCTCCATCGTTTAAGGAGCAT
GCGACAAATGAAAACAGAGGTGAAGTAGCCAGTAAACCCAGTGAACTTGTTTCTGGTTCT
AACAGCATAATTTCTTTCAGCAGTGTCATAAGTATGGCTAAACCCATTGGTCTATGCATG
CCTAACAGTGCCCACATCATGGCCAGCAATACCATGCAAGTGGAAGAGCCACCCAAGTCT
CCTGAGAAAATGTGGCCTAAAGATGAAAATTTTGAACAGAGAAGCTCATTGGAGTGTGCT
CAGAAGGCTGCAGAGCTTCGAGCTTCCATTAAAGAGAAGGTAGAGCTGATTCGACTTAAG
AAGCTCTTACATGAAAGAAATGCTTCATTGGTTATGACAAAAGCACAATTAACAGAAGTT
CAAGAGGCATACGAAACCTTGCTCCAGAAGAATCAGGGAATCCTGAGTGCAGCCCATGAG
GCCCTCCTCAAGCAAGTGAATGAGCTCAGGGCAGAGCTGAAGGAAGAAAGCAAGAAGGCT
GTGAGCTTGAAGAGCCAACTGGAAGATGTGTCTATCTTGCAGATGACTCTGAAGGAGTTT
CAGGAGAGAGTTGAAGATTTGGAAAAAGAACGAAAATTGCTGAATGACAATTATGACAAA
CTCTTAGAAAGCATGCTGGACAGCAGTGACAGCTCCAGTCAGCCCCACTGGAGCAACGAG
CTCATAGCGGAACAGCTACAGCAGCAAGTCTCTCAGCTGCAGGATCAGCTGGATGCTGAG
CTGGAGGACAAGAGAAAAGTTTTACTTGAGCTGTCCAGGGAGAAAGCCCAAAATGAGGAT
CTGAAGCTTGAAGTCACCAACATACTTCAGAAGCATAAACAGGAAGTAGAGCTCCTCCAA
AATGCAGCCACAATTTCCCAACCTCCTGACAGGCAATCTGAACCAGCCACTCACCCAGCT
GTATTGCAAGAGAACACTCAGATCGAGCCAAGTGAACCCAAAAACCAAGAAGAAAAGAAA
CTGTCCCAGGTGCTAAATGAGTTGCAAGTATCACACGCAGAGACCACATTGGAACTAGAA
AAGACCAGGGACATGCTTATTCTGCAGCGCAAAATCAACGTGTGTTATCAGGAGGAACTG
GAGGCAATGATGACAAAAGCTGACAATGATAATAGAGATCACAAAGAAAAGCTGGAGAGG
TTGACTCGACTACTAGACCTCAAGAATAACCGTATCAAGCAGCTGGAAGGTATTTTAAGA
AGCCATGACCTTCCAACATCTGAACAGCTCAAAGATGTTGCTTATGGCACCCGACCGTTG
TCGTTATGTTTGGAAACACTGCCAGCCCATGGAGATGAGGATAAAGTGGATATTTCTCTG
CTGCATCAGGGTGAGAATCTTTTTGAACTGCACATCCACCAGGCCTTCCTGACATCTGCC
GCCCTAGCTCAGGCTGGAGATACCCAACCTACCACTTTCTGCACCTATTCCTTCTATGAC
TTTGAAACCCACTGTACCCCATTATCTGTGGGGCCACAGCCCCTCTATGACTTCACCTCC
CAGTATGTGATGGAGACAGATTCGCTTTTCTTACACTACCTTCAAGAGGCTTCAGCCCGG
CTTGACATACACCAGGCCATGGCCAGTGAACACAGCACTCTTGCTGCAGGATGGATTTGC
TTTGACAGGGTGCTAGAGACTGTGGAGAAAGTCCATGGCTTGGCCACACTGATTGGAGCT
GGTGGAGAAGAGTTCGGGGTTCTAGAGTACTGGATGAGGCTGCGTTTCCCCATAAAACCC
AGCCTACAGGCGTGCAATAAACGAAAGAAAGCCCAGGTCTACCTGTCAACCGATGTGCTT
GGAGGCCGGAAGGCCCAGGAAGAGGAGTTCAGATCGGAGTCTTGGGAACCTCAGAACGAG
CTGTGGATTGAAATCACCAAGTGCTGTGGCCTCCGGAGTCGATGGCTGGGAACTCAACCC
AGTCCATATGCTGTGTACCGCTTCTTCACCTTTTCTGACCATGACACTGCCATCATTCCA
GCCAGTAACAACCCCTACTTTAGAGACCAGGCTCGATTCCCAGTGCTTGTGACCTCTGAC
CTGGACCATTATCTGAGACGGGAGGCCTTGTCTATACATGTTTTTGATGATGAAGACTTA
GAGCCTGGCTCGTATCTTGGCCGAGCCCGAGTGCCTTTACTGCCTCTTGCAAAAAATGAA
TCTATCAAAGGTGATTTTAACCTCACTGACCCTGCAGAGAAACCCAACGGATCTATTCAA
GTGCAACTGGATTGGAAGTTTCCCTACATACCCCCTGAGAGCTTCCTGAAACCAGAAGCT
CAGACTAAGGGGAAGGATACCAAGGACAGTTCAAAGATCTCATCTGAAGAGGAAAAGGCT
TCATTTCCTTCCCAGGATCAGATGGCATCTCCTGAGGTTCCCATTGAAGCTGGCCAGTAT
CGATCTAAGAGAAAACCTCCTCATGGGGGAGAAAGAAAGGAGAAGGAGCACCAGGTTGTG
AGCTACTCAAGAAGAAAACATGGCAAAAGAATAGGTGTTCAAGGAAAGAATAGAATGGAG
TATCTTAGCCTTAACATCTTAAATGGAAATACACCACAGCAGGTGAATTACACTGAGTGG
AAGTTCTCAGAGACTAACAGCTTCATAGGTGATGGCTTTAAAAATCAGCACGAGGAAGAG
GAAATGACATTATCCCATTCAGCACTGAAACAGAAGGAACCTCTACATCCTGTAAATGAC
AAAGAATCCTCTGAACAAGGTTCTGAAGTCAGTGAAGCACAAACTACCGACAGTGATGAT
GTCATAGTGCCACCCATGTCTCAGAAATATCCTAAGGCAGATTCAGAGAAGATGTGCATT
GAAATTGTCTCCCTGGCCTTCTACCCAGAGGCAGAAGTGATGTCTGATGAGAACATAAAA
CAGGTGTATGTGGAGTACAAATTCTACGACCTACCCTTGTCGGAGACAGAGACTCCAGTG
TCCCTAAGGAAGCCTAGGGCAGGAGAAGAAATCCACTTTCACTTTAGCAAGGTAATAGAC
CTGGACCCACAGGAGCAGCAAGGCCGAAGGCGGTTTCTGTTCGACATGCTGAATGGACAA
GATCCTGATCAAGGACATTTAAAGTTTACAGTGGTAAGTGATCCTCTGGATGAAGAAAAG
AAAGAATGTGAAGAAGTGGGATATGCATATCTTCAACTGTGGCAGATCCTGGAGTCAGGA
AGAGATATTCTAGAGCAAGAGCTAGACATTGTTAGCCCTGAAGATCTGGCTACCCCAATA
GGAAGGCTGAAGGTTTCCCTTCAAGCAGCTGCTGTCCTCCATGCTATTTACAAGGAGATG
ACTGAAGATTTGTTTTCATGA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_020366 unedited
GGGTTCACGATTTTGTATACGACTCACTATAGGGACGGCCGCGAAATCATGTCACATCTG
GTGGACCCTACATCAGGAGACTTGCCAGTTAGAGACATAGATGCTATACCTCTGGTGCTA
CCAGCCTCAAAAGGTAAGAATATGAAAACTCAACCACCCTTGAGCAGGATGAACCGGGAG
GAATTGGAGGACAGTTTCTTTCGACTTCGCGAAGATCACATGTTGGTGAAGGAGCTTTCT
TGGAAGCAACAGGATGAGATCAAAAGGCTGAGGACCACCTTGCTGCGGTTGACCGCTGCT
GGCCGGGACCTGCGGGTCGCGGAGGAGGCGGCGCCGCTCTCGGAGACCGCAAGGCGCGGG
CAGAAGGCGGGATGGCGGCAGCGCCTCTCCATGCACCAGCGCCCCCAGATGCACCGACTG
CAAGGGCATTTCCACTGCGTCGGCCCTGCCAGCCCCCGCCGCGCCCAGCCTCGCGTCCAA
GTGGGACACAGACAGCTCCACACAGCCGGTGCACCGGTGCCGGAGAAACCCAAGAGGGGG
CCAAGGGACAGGCTGAGCTACACAGCCCCTCCATCGTTTAAGGAGCATGCGACAAATGAA
AACAGAGGTGAAGTAGCCAGTAAACCCAGTGAACTTGTTTCTGGTTCTAACAGCATAATT
TCTTTCAGCAGTGTCATAAGTATGGCTAAACCCATTGGTCTATGCATGCCTAACAGTGCC
CACATCATGGCCAGCAATACCATGCAAGTGGAAGAGCCACCCAAGTCTCCTGAGAAAATG
TGGCCTANAGATGAAATTTTGAACAGAGAAGCTCATTGGAGTGTGCTCAGAAGGCTGCTG
AGCTTCGAGCTTCCATTTAAGAGAAGTAGAGCTGATTCGACTTAAGAAGCTCTTACT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_020366 unedited
NGGGCCATTGGANGATGGCACTTNCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGG
ATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCCCTTTTTTTTTTTTT
TTTTTTTTTTTTTTCATGTTATAGCAAGCTAACTTTATAAAAGACTTTTTACTATGGTTC
CCTCAAAGACTTTTAAATTGGAATAGCACTTGTTCCTTCATGAAAACAAATCTTCAGTCA
TCTCCTTGTAAATAGCATGGAGGACAGCAGCTGCTTGAAGGGAAACCTTCAGCCTTCCTA
TTGGGGTAGCCAGATCTTCAGGGCTAACAATGTCTAGCTCTTGCTCTAAAATATCTCTTC
CTGACTCCAGGATCTGCCACAGTTGAAAATATGCATATCCCACTTCTTCACATTCTTTCT
TTTCTTCATCCAGAGGATCACTTACCACTGTAAACTTTAAATGTCCTTGATCAGGATCTT
GTCCATTCAGCATGTCGAACAGAAACCGCCTTCGGCCTTGCTGCTCCTGTGGGTCCAGGT
CTATTACCTTGCTAAAGTGAAAGTGGATTTCTTCTCCTGCCCTAGGCTTCCTTAGGGACA
CTGGAGTCTCTGTCTCCGACAAGGGTAGGTCGTAAAATTTGTACTCCACATACACCTGTT
TTATGTTCTCATCAGACATCACTTCTGCCTCTGGGTAGAAGGCCAGGGAGACAATTTCAA
TGCACATCTTCTCTGAATCTGCCTTAGGATATTTCTGAGACATGGGTGGCACTATGACAT
CATCACTGTCGGTAGTTTGTGCTTCACTGACTTCAGAACCTTGTTCAGAGGATTCTTTGT
CATTTACAGGATGTANNAGGTTCCTTCTGTTTTCAGGCTGAATGGGATN
Restriction Sites Please inquire |
ACCN NM_020366
Insert Size 3861 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020366.2, NP_065099.2
RefSeq Size 3861 bp
RefSeq ORF 3861 bp
Locus ID 57096
UniProt ID Q96KN7
Cytogenetics 14q11.2
Protein Families Druggable Genome
Summary This gene encodes a photoreceptor protein that interacts with retinitis pigmentosa GTPase regulator protein and is a key component of cone and rod photoreceptor cells. Mutations in this gene lead to autosomal recessive congenital blindness. provided by RefSeq, Oct 2008
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"NM_020366" in other vectors (6)
SKU Description Size Price
RC217420 RPGRIP1 (Myc-DDK-tagged)-Human retinitis pigmentosa GTPase regulator interacting protein 1 (RPGRIP1) 10 ug
$1,119.00
RC217420L1 Lenti ORF clone of Human retinitis pigmentosa GTPase regulator interacting protein 1 (RPGRIP1), Myc-DDK-tagged 10 ug
$1,419.00
RC217420L2 Lenti ORF clone of Human retinitis pigmentosa GTPase regulator interacting protein 1 (RPGRIP1), mGFP tagged 10 ug
$1,419.00
RC217420L3 Lenti ORF clone of Human retinitis pigmentosa GTPase regulator interacting protein 1 (RPGRIP1), Myc-DDK-tagged 10 ug
$1,419.00
RC217420L4 Lenti ORF clone of Human retinitis pigmentosa GTPase regulator interacting protein 1 (RPGRIP1), mGFP tagged 10 ug
$1,419.00
RG217420 RPGRIP1 (tGFP-tagged) - Human retinitis pigmentosa GTPase regulator interacting protein 1 (RPGRIP1) 10 ug
$789.00 $1,319.00

file-alt Citations

Replacement gene therapy with a human RPGRIP1 sequence slows photoreceptor degeneration in a murine model of Leber congenital amaurosis
Pawlyk, Bulgakov, Liu et al
Hum Gene Ther (2010) 21 (8), 993-1004 DOI: 10.1089/hum.2009.218
Cited by 79
AI Summary: In this study, OriGene’s human RPGRIP1 cDNA clone (Cat # RC221091) was used as the source of the replacement gene for constructing the gene therapy vector, enabling the evaluation of human RPGRIP1 gene therapy in a murine model of Leber congenital amaurosis.
Showing 1-1 of 1 papers.
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