KRT72 (NM_080747) Human Tagged ORF Clone

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SKU
RC216910
KRT72 (Myc-DDK-tagged)-Human keratin 72 (KRT72), transcript variant 1
  $476.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KRT72
Synonyms CK-72; K6irs; K6IRS2; K72; KRT6; KRT6IRS2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216910 representing NM_080747
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCGCCAACTGACCCATTTCCCCCGCGGGGAGCGCCTGGGCTTCAGCGGTTGCTCCGCGGTCCTCT
CTGGCGGGATCGGCAGCAGCTCCGCCTCATTCCGGGCCCGGGTCAAGGGCTCGGCCTCCTTTGGCAGCAA
GAGCCTCTCCTGCCTTGGGGGCAGCCGAAGCCTGGCGCTCAGCGCTGCTGCACGGCGGGGCGGCGGCCGC
CTGGGCGGCTTCGTGGGCACCGCCTTCGGCAGCGCCGGGCTGGGGCCCAAGTGTCCCTCCGTGTGCCCAC
CCGGGGGCATCCCTCAGGTCACCGTCAACAAGAGCCTCCTGGCCCCGCTCAACGTGGAGATGGACCCCGA
GATCCAGAGGGTGCGCGCCCAGGAGCGGGAGCAGATCAAGGCGCTAAACAACAAGTTCGCCTCCTTCATC
GACAAGGTGCGGTTCCTGGAGCAGCAGAATCAGGTGCTAGAGACCAAGTGGAACCTCCTACAGCAGCTGG
ACTTGAACAACTGCAGGAAGAACCTGGAGCCCATTTATGAGGGCTACATCAGCAACCTGCAGAAGCAGCT
GGAGATGCTGTCTGGGGACGGGGTGAGGCTGGATTCGGAGCTGAGGAACATGCAGGATTTGGTGGAGGAC
TACAAGAAGAGGTATGAGGTGGAGATTAACAGACGCACAGCTGCTGAGAATGAGTTTGTGGTGCTCAAGA
AGGACGTGGATGCTGCTTACATGAATAAGGTTGAGCTCCAGGCCAAGGTGGACTCCTTGACAGATGAGAT
TAAATTCTTCAAGTGCCTTTATGAAGGGGAGATCACTCAGATCCAGTCCCACATCAGCGACACGTCCATC
GTCCTGTCAATGGACAACAACCGGGATCTGGACCTGGACAGCATCATTGCCGAGGTCCGTGCCCAGTACG
AGGAGATTGCCCTAAAGAGCAAGGCCGAGGCTGAGACCCTGTACCAGACCAAGATCCAGGAGCTGCAGGT
CACAGCAGGCCAGCATGGGGATGACCTCAAGCTCACCAAGGCTGAAATCTCTGAGCTCAACCGCCTGATC
CAGAGGATCCGCTCAGAGATAGGGAATGTGAAGAAGCAGTGTGCCGATCTGGAGACGGCCATCGCCGACG
CTGAACAGCGGGGGGACTGCGCCCTGAAAGATGCCCGGGCCAAGCTGGATGAGCTGGAGGGCGCCCTGCA
CCAGGCCAAGGAGGAGCTGGCACGGATGCTGCGTGAGTACCAGGAGCTCGTGAGCCTGAAGCTGGCCCTG
GATATGGAGATCGCCACCTACCGCAAGCTGCTGGAGAGCGAGGAGTGCAGGATGTCTGGCGAATATCCAA
ATTCTGTGAGCATCTCCGTCATCAGCAGCACCAATGCTGGGGCAGGAGGGGCTGGCTTCAGCATGGGCTT
TGGCGCCTCAAGCAGTTATAGCTACAAAACTGCAGCTGCAGACGTCAAGACCAAAGGCAGCTGTGGCAGT
GAGCTCAAGGATCCCCTTGCCAAAACCTCGGGGAGCAGCTGTGCCACCAAAAAGGCCTCCAGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC216910 representing NM_080747
Red=Cloning site Green=Tags(s)

MSRQLTHFPRGERLGFSGCSAVLSGGIGSSSASFRARVKGSASFGSKSLSCLGGSRSLALSAAARRGGGR
LGGFVGTAFGSAGLGPKCPSVCPPGGIPQVTVNKSLLAPLNVEMDPEIQRVRAQEREQIKALNNKFASFI
DKVRFLEQQNQVLETKWNLLQQLDLNNCRKNLEPIYEGYISNLQKQLEMLSGDGVRLDSELRNMQDLVED
YKKRYEVEINRRTAAENEFVVLKKDVDAAYMNKVELQAKVDSLTDEIKFFKCLYEGEITQIQSHISDTSI
VLSMDNNRDLDLDSIIAEVRAQYEEIALKSKAEAETLYQTKIQELQVTAGQHGDDLKLTKAEISELNRLI
QRIRSEIGNVKKQCADLETAIADAEQRGDCALKDARAKLDELEGALHQAKEELARMLREYQELVSLKLAL
DMEIATYRKLLESEECRMSGEYPNSVSISVISSTNAGAGGAGFSMGFGASSSYSYKTAAADVKTKGSCGS
ELKDPLAKTSGSSCATKKASR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080747
ORF Size 1533 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080747.3
RefSeq Size 1985 bp
RefSeq ORF 1536 bp
Locus ID 140807
UniProt ID Q14CN4
Cytogenetics 12q13.13
MW 55.7 kDa
Summary Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. The type II keratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This gene encodes a type II keratin that is specifically expressed in the inner root sheath of hair follicles. The type II keratins are clustered in a region of chromosome 12q12-q13. Multiple transcript variants encoding different isoforms have been found for this gene.provided by RefSeq, Jun 2009
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Documents
Resources
"NM_080747" in other vectors (4)
SKU Description Size Price
RC216910L3 Lenti ORF clone of Human keratin 72 (KRT72), transcript variant 1, Myc-DDK-tagged 10 ug
$776.00
RC216910L4 Lenti ORF clone of Human keratin 72 (KRT72), transcript variant 1, mGFP tagged 10 ug
$776.00
RG216910 KRT72 (tGFP-tagged) - Human keratin 72 (KRT72), transcript variant 1 10 ug
$676.00
SC305827 KRT72 (untagged)-Human keratin 72 (KRT72), transcript variant 1 10 ug
$524.00

Plasmid Map for RC216910

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Cloning scheme for RC216910

Circular map for RC216910

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