KRT72 (NM_080747) Human Untagged Clone

SKU
SC305827
KRT72 (untagged)-Human keratin 72 (KRT72), transcript variant 1
  $524.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol KRT72
Synonyms CK-72; K6irs; K6IRS2; K72; KRT6; KRT6IRS2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC305827 representing NM_080747.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAGCCGCCAACTGACCCATTTCCCCCGCGGGGAGCGCCTGGGCTTCAGCGGTTGCTCCGCGGTCCTC
TCTGGCGGGATCGGCAGCAGCTCCGCCTCATTCCGGGCCCGGGTCAAGGGCTCGGCCTCCTTTGGCAGC
AAGAGCCTCTCCTGCCTTGGGGGCAGCCGAAGCCTGGCGCTCAGCGCTGCTGCACGGCGGGGCGGCGGC
CGCCTGGGCGGCTTCGTGGGCACCGCCTTCGGCAGCGCCGGGCTGGGGCCCAAGTGTCCCTCCGTGTGC
CCACCCGGGGGCATCCCTCAGGTCACCGTCAACAAGAGCCTCCTGGCCCCGCTCAACGTGGAGATGGAC
CCCGAGATCCAGAGGGTGCGCGCCCAGGAGCGGGAGCAGATCAAGGCGCTAAACAACAAGTTCGCCTCC
TTCATCGACAAGGTGCGGTTCCTGGAGCAGCAGAATCAGGTGCTAGAGACCAAGTGGAACCTCCTACAG
CAGCTGGACTTGAACAACTGCAGGAAGAACCTGGAGCCCATTTATGAGGGCTACATCAGCAACCTGCAG
AAGCAGCTGGAGATGCTGTCTGGGGACGGGGTGAGGCTGGATTCGGAGCTGAGGAACATGCAGGATTTG
GTGGAGGACTACAAGAAGAGGTATGAGGTGGAGATTAACAGACGCACAGCTGCTGAGAATGAGTTTGTG
GTGCTCAAGAAGGACGTGGATGCTGCTTACATGAATAAGGTTGAGCTCCAGGCCAAGGTGGACTCCTTG
ACAGATGAGATTAAATTCTTCAAGTGCCTTTATGAAGGGGAGATCACTCAGATCCAGTCCCACATCAGC
GACACGTCCATCGTCCTGTCAATGGACAACAACCGGGATCTGGACCTGGACAGCATCATTGCCGAGGTC
CGTGCCCAGTACGAGGAGATTGCCCTAAAGAGCAAGGCCGAGGCTGAGACCCTGTACCAGACCAAGATC
CAGGAGCTGCAGGTCACAGCAGGCCAGCATGGGGATGACCTCAAGCTCACCAAGGCTGAAATCTCTGAG
CTCAACCGCCTGATCCAGAGGATCCGCTCAGAGATAGGGAATGTGAAGAAGCAGTGTGCCGATCTGGAG
ACGGCCATCGCCGACGCTGAACAGCGGGGGGACTGCGCCCTGAAAGATGCCCGGGCCAAGCTGGATGAG
CTGGAGGGCGCCCTGCACCAGGCCAAGGAGGAGCTGGCACGGATGCTGCGTGAGTACCAGGAGCTCGTG
AGCCTGAAGCTGGCCCTGGATATGGAGATCGCCACCTACCGCAAGCTGCTGGAGAGCGAGGAGTGCAGG
ATGTCTGGCGAATATCCAAATTCTGTGAGCATCTCCGTCATCAGCAGCACCAATGCTGGGGCAGGAGGG
GCTGGCTTCAGCATGGGCTTTGGCGCCTCAAGCAGTTATAGCTACAAAACTGCAGCTGCAGACGTCAAG
ACCAAAGGCAGCTGTGGCAGTGAGCTCAAGGATCCCCTTGCCAAAACCTCGGGGAGCAGCTGTGCCACC
AAAAAGGCCTCCAGATGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_080747
Insert Size 1536 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080747.2
RefSeq Size 2030 bp
RefSeq ORF 1536 bp
Locus ID 140807
UniProt ID Q14CN4
Cytogenetics 12q13.13
MW 55.9 kDa
Summary Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. The type II keratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This gene encodes a type II keratin that is specifically expressed in the inner root sheath of hair follicles. The type II keratins are clustered in a region of chromosome 12q12-q13. Multiple transcript variants encoding different isoforms have been found for this gene.provided by RefSeq, Jun 2009
Transcript Variant: This variant (1) represents the predominant transcript and encodes the longer isoform (1). Variants 1 and 2 encode the same protein.
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Documents
Resources
"NM_080747" in other vectors (4)
SKU Description Size Price
RC216910 KRT72 (Myc-DDK-tagged)-Human keratin 72 (KRT72), transcript variant 1 10 ug
$476.00
RC216910L3 Lenti ORF clone of Human keratin 72 (KRT72), transcript variant 1, Myc-DDK-tagged 10 ug
$776.00
RC216910L4 Lenti ORF clone of Human keratin 72 (KRT72), transcript variant 1, mGFP tagged 10 ug
$776.00
RG216910 KRT72 (tGFP-tagged) - Human keratin 72 (KRT72), transcript variant 1 10 ug
$676.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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