ANKLE2 (NM_015114) Human Tagged ORF Clone
$859.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | ANKLE2 |
| Synonyms | KIAA0692; Lem4; LEMD7; MCPH16 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_015114 |
| ORF Size | 2814 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_015114.3 |
| RefSeq Size | 4491 bp |
| RefSeq ORF | 2817 bp |
| Locus ID | 23141 |
| UniProt ID | Q86XL3 |
| Cytogenetics | 12q24.33 |
| Protein Families | Transmembrane |
| MW | 104.1 kDa |
| Summary | This gene encodes a member of the LEM family of inner nuclear membrane proteins. The encoded protein functions as a mitotic regulator through postmitotic formation of the nuclear envelope. Mutations in this gene cause morphology defects in the nuclear envelope and BAF hyperphosphorylation. provided by RefSeq, Mar 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_015114" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC216867L1 | Lenti-ORF clone of ANKLE2 (Myc-DDK-tagged)-Human ankyrin repeat and LEM domain containing 2 (ANKLE2) | 10 ug |
$1,159.00
|
|
| RC216867L2 | Lenti-ORF clone of ANKLE2 (mGFP-tagged)-Human ankyrin repeat and LEM domain containing 2 (ANKLE2) | 10 ug |
$1,159.00
|
|
| RC216867L3 | Lenti-ORF clone of ANKLE2 (Myc-DDK-tagged)-Human ankyrin repeat and LEM domain containing 2 (ANKLE2) | 10 ug |
$1,159.00
|
|
| RC216867L4 | Lenti-ORF clone of ANKLE2 (mGFP-tagged)-Human ankyrin repeat and LEM domain containing 2 (ANKLE2) | 10 ug |
$1,159.00
|
|
| RG216867 | ANKLE2 (tGFP-tagged) - Human ankyrin repeat and LEM domain containing 2 (ANKLE2) | 10 ug |
$789.00
$1,059.00
|
|
| SC316434 | ANKLE2 (untagged)-Human ankyrin repeat and LEM domain containing 2 (ANKLE2) | 10 ug |
$946.00
|
|
Citations
LEM4 confers tamoxifen resistance to breast cancer cells by activating cyclin D-CDK4/6-Rb and ERα pathway
Gao, Sun, Ma et al
Nat Commun (2018) 9 (1), 4180 DOI: 10.1038/s41467-018-06309-8
Gao, Sun, Ma et al
Nat Commun (2018) 9 (1), 4180 DOI: 10.1038/s41467-018-06309-8
AI Summary: OriGene products pCMV6-FLAG-LEM4 and pCMV6-FLAG-CDK4 plasmids were used to overexpress LEM4 and CDK4 in breast cancer cells, enabling the study of their roles in tamoxifen resistance.
Snippet: The pCMV6-FLAG-LEM4 and pCMV6-FLAG-CDK4 plasmids were purchased from OriGene. Mammalian expression plasmids for GFP-Aurora-A and GFP-ERα were generated in our laboratory. The mammalian expression plasmids for various ERα mutants were constructed by PCR amplification using the primers listed in Supplementary Table 2. …
Showing 1-1 of 1 papers.
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