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ANKLE2 (NM_015114) Human Untagged Clone

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SKU
SC316434
ANKLE2 (untagged)-Human ankyrin repeat and LEM domain containing 2 (ANKLE2)
  $946.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ANKLE2
Synonyms KIAA0692; Lem4; LEMD7; MCPH16
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC316434 representing NM_015114.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCTGTGGCCGCGGCTGGCGGCGGCCGAGTGGGCGGCGCTGGCCTGGGAGCTGCTGGGCGCCTCGGTG
CTGCTGATCGCTGTGCGGTGGCTGGTGCGGCGGCTGGGCCCGCGGCCGGGAGGTCTGGGCCGCAGCGGG
ACCCCAGTTCCTCCGCCAAGCGCGGCCGCCGCCCCCGCCTCAGGTGAAATGACAATGGATGCTCTGTTG
GCTCGATTGAAACTTCTGAATCCAGATGACCTTAGAGAAGAAATCGTCAAAGCCGGATTGAAATGTGGA
CCCATTACATCAACTACAAGGTTCATTTTTGAGAAAAAATTGGCTCAGGCTTTACTGGAGCAAGGAGGA
AGGCTGTCTTCTTTCTACCACCATGAGGCAGGTGTCACAGCTCTCAGCCAGGACCCACAAAGGATTTTG
AAGCCAGCTGAAGGGAACCCAACTGATCAGGCTGGTTTTTCTGAAGACAGAGATTTTGGTTACAGTGTG
GGCCTGAATCCTCCAGAGGAGGAAGCTGTGACATCCAAGACCTGCTCGGTGCCCCCTAGTGACACCGAC
ACCTACAGAGCTGGAGCGACTGCGTCTAAGGAGCCGCCCCTGTACTATGGGGTGTGTCCAGTGTATGAG
GACGTCCCAGCGAGAAATGAAAGGATCTATGTTTATGAAAATAAAAAGGAAGCATTGCAAGCTGTCAAG
ATGATCAAAGGGTCCCGATTTAAAGCTTTTTCTACCAGAGAAGACGCTGAGAAATTTGCTAGAGGAATT
TGTGATTATTTCCCTTCTCCAAGCAAAACGTCCTTACCACTGTCTCCTGTGAAAACAGCTCCACTCTTT
AGCAATGACAGGTTGAAAGATGGTTTGTGCTTGTCGGAATCAGAAACAGTCAACAAAGAGCGAGCGAAC
AGTTACAAAAATCCCCGCACGCAGGACCTCACCGCCAAGCTTCGGAAAGCTGTGGAGAAGGGAGAGGAG
GACACCTTTTCTGACCTTATCTGGAGCAACCCCCGGTATCTGATAGGCTCAGGAGACAACCCCACTATC
GTGCAGGAAGGGTGCAGGTACAACGTGATGCATGTTGCTGCCAAAGAGAACCAGGCTTCCATCTGCCAG
CTGACTCTGGACGTCCTGGAGAACCCTGACTTCATGAGGCTGATGTACCCTGATGACGACGAGGCCATG
CTGCAGAAGCGTATCCGTTACGTGGTGGACCTGTACCTCAACACCCCCGACAAGATGGGCTATGACACA
CCGTTGCATTTTGCTTGTAAGTTTGGAAATGCAGATGTAGTCAACGTGCTTTCGTCACACCATTTGATT
GTAAAAAACTCAAGGAATAAATATGATAAAACACCTGAAGATGTAATTTGTGAAAGAAGCAAAAATAAA
TCTGTGGAACTGAAGGAGCGGATCAGAGAGTATTTAAAGGGCCACTACTACGTGCCCCTCCTGAGAGCG
GAAGAGACTTCTTCTCCAGTCATCGGGGAGCTGTGGTCCCCAGACCAGACGGCTGAGGCCTCTCACGTC
AGCCGCTATGGAGGCAGCCCCAGAGACCCGGTACTGACCCTGAGAGCCTTCGCAGGGCCCCTGAGTCCA
GCCAAGGCAGAAGATTTTCGCAAGCTCTGGAAAACTCCACCTCGAGAGAAAGCAGGCTTCCTTCACCAC
GTCAAGAAGTCGGACCCGGAAAGAGGCTTTGAGAGAGTGGGAAGGGAGCTAGCTCATGAGCTGGGGTAT
CCCTGGGTTGAATACTGGGAATTTCTGGGCTGTTTTGTTGATCTGTCTTCCCAGGAAGGCCTGCAAAGA
CTAGAAGAATATCTCACACAGCAGGAAATAGGCAAAAAGGCTCAACAAGAAACAGGAGAACGGGAAGCC
TCCTGCCGAGATAAAGCCACCACGTCTGGCAGCAATTCCATTTCCGTGAGGGCGTTTCTAGATGAAGAT
GACATGAGCTTGGAAGAAATAAAAAATCGGCAAAATGCAGCTCGAAATAACAGCCCGCCCACAGTCGGT
GCTTTTGGACATACGAGGTGCAGCGCCTTCCCCTTGGAGCAGGAGGCAGACCTCATAGAAGCCGCCGAG
CCGGGAGGTCCACACAGCAGCAGAAATGGGCTCTGCCATCCTCTGAATCACAGCAGGACCCTGGCGGGC
AAGAGACCAAAGGCCCCCCGTGGGGAGGAAGCCCATCTGCCACCTGTCTCGGATTTGACTGTTGAGTTT
GATAAACTGAATTTGCAAAATATAGGACGTAGCGTTTCCAAGACACCAGATGAAAGTACAAAAACTAAA
GATCAGATCCTGACTTCAAGAATCAATGCAGTAGAAAGAGACTTGTTAGAGCCTTCTCCCGCAGACCAA
CTCGGGAATGGCCACAGGAGGACAGAAAGTGAAATGTCAGCCAGGATCGCTAAAATGTCCTTGAGTCCC
AGCAGCCCCAGGCACGAGGATCAGCTCGAGGTCACCAGGGAACCGGCCAGGCGGCTCTTCCTTTTTGGA
GAGGAGCCATCAAAACTCGATCAGGATGTTTTGGCCGCTCTTGAATGTGCAGACGTCGACCCCCATCAG
TTCCCGGCCGTGCACAGATGGAAGAGTGCTGTCCTGTGCTACTCACCCTCGGACAGACAGAGTTGGCCC
AGTCCCGCGGTGAAAGGAAGGTTCAAGTCTCAGCTGCCAGATCTCAGTGGCCCTCACAGCTACAGTCCG
GGGAGAAACAGCGTGGCTGGAAGCAACCCCGCAAAGCCAGGCCTGGGCAGTCCTGGGCGCTACAGCCCC
GTGCACGGGAGCCAGCTCCGCAGGATGGCGCGCCTGGCTGAGCTTGCCGCCCTGTAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_015114
Insert Size 2817 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015114.2
RefSeq Size 4499 bp
RefSeq ORF 2817 bp
Locus ID 23141
UniProt ID Q86XL3
Cytogenetics 12q24.33
Protein Families Transmembrane
MW 104.1 kDa
Summary This gene encodes a member of the LEM family of inner nuclear membrane proteins. The encoded protein functions as a mitotic regulator through postmitotic formation of the nuclear envelope. Mutations in this gene cause morphology defects in the nuclear envelope and BAF hyperphosphorylation. provided by RefSeq, Mar 2014
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"NM_015114" in other vectors (6)
SKU Description Size Price
RC216867 ANKLE2 (Myc-DDK-tagged)-Human ankyrin repeat and LEM domain containing 2 (ANKLE2) 10 ug
$859.00
RC216867L1 Lenti-ORF clone of ANKLE2 (Myc-DDK-tagged)-Human ankyrin repeat and LEM domain containing 2 (ANKLE2) 10 ug
$1,159.00
RC216867L2 Lenti-ORF clone of ANKLE2 (mGFP-tagged)-Human ankyrin repeat and LEM domain containing 2 (ANKLE2) 10 ug
$1,159.00
RC216867L3 Lenti-ORF clone of ANKLE2 (Myc-DDK-tagged)-Human ankyrin repeat and LEM domain containing 2 (ANKLE2) 10 ug
$1,159.00
RC216867L4 Lenti-ORF clone of ANKLE2 (mGFP-tagged)-Human ankyrin repeat and LEM domain containing 2 (ANKLE2) 10 ug
$1,159.00
RG216867 ANKLE2 (tGFP-tagged) - Human ankyrin repeat and LEM domain containing 2 (ANKLE2) 10 ug
$789.00 $1,059.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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