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CDCA7 (NM_031942) Human Tagged ORF Clone

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SKU
RC216735
CDCA7 (Myc-DDK-tagged)-Human cell division cycle associated 7 (CDCA7), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CDCA7
Synonyms ICF3; JPO1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216735 representing NM_031942
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGCTCGCCGCGTGCCGCAGAAAGATCTCAGAGTAAAGAAGAACTTAAAGAAATTCAGATATGTGA
AGTTGATTTCCATGGAAACCTCGTCATCCTCTGATGACAGTTGTGACAGCTTTGCTTCTGATAATTTTGC
AAACACGAAACCTAAATTCAGGTCAGATATCAGTGAAGAACTGGCAAATGTTTTTTATGAGGACTCTGAT
AATGAATCTTTCTGCGGCTTTTCAGAAAGTGAGGTGCAAGATGTATTAGACCATTGTGGATTTTTACAGA
AACCAAGGCCAGATGTCACTAACGAACTGGCCGGTATTTTTCATGCCGACTCTGACGATGAATCATTTTG
CGGTTTCTCAGAGAGTGAGATACAAGATGGAATGAGGCTGCAGTCAGTTCGGGAAGGCTGTAGGACCCGC
AGCCAGTGCAGGCACTCTGGACCTCTCAGGGTGGCGATGAAGTTTCCAGCGCGGAGTACCAGGGGAGCAA
CCAACAAAAAAGCAGAGTCCCGCCAGCCCTCAGAGAATTCTGTGACTGATTCCAACTCCGATTCAGAAGA
TGAAAGTGGAATGAATTTTTTGGAGAAAAGGGCTTTAAATATAAAGCAAAACAAAGCAATGCTTGCAAAA
CTCATGTCTGAATTAGAAAGCTTCCCTGGCTCGTTCCGTGGAAGACATCCCCTCCCAGGCTCCGACTCAC
AATCAAGGAGACCGCGAAGGCGTACATTCCCGGGTGTTGCTTCCAGGAGAAACCCTGAACGGAGAGCTCG
TCCTCTTACCAGGTCAAGGTCCCGGATCCTCGGGTCCCTTGACGCTCTACCCATGGAGGAGGAGGAGGAA
GAGGATAAGTACATGTTGGTGAGAAAGAGGAAGACCGTGGATGGCTACATGAATGAAGATGACCTGCCCA
GAAGCCGTCGCTCCAGATCATCCGTGACCCTTCCGCATATAATTCGCCCAGTGGAAGAAATTACAGAGGA
GGAGTTGGAGAACGTCTGCAGCAATTCTCGAGAGAAGATATATAACCGTTCACTGGGCTCTACTTGTCAT
CAATGCCGTCAGAAGACTATTGATACCAAAACAAACTGCAGAAACCCAGACTGCTGGGGCGTTCGAGGCC
AGTTCTGTGGCCCCTGCCTTCGAAACCGTTATGGTGAAGAGGTCAGGGATGCTCTGCTGGATCCGAACTG
GCATTGCCCGCCTTGTCGAGGAATCTGCAACTGCAGTTTCTGCCGGCAGCGAGATGGACGGTGTGCGACT
GGGGTCCTTGTGTATTTAGCCAAATATCATGGCTTTGGGAATGTGCATGCCTACTTGAAAAGCCTGAAAC
AGGAATTTGAAATGCAAGCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC216735 representing NM_031942
Red=Cloning site Green=Tags(s)

MDARRVPQKDLRVKKNLKKFRYVKLISMETSSSSDDSCDSFASDNFANTKPKFRSDISEELANVFYEDSD
NESFCGFSESEVQDVLDHCGFLQKPRPDVTNELAGIFHADSDDESFCGFSESEIQDGMRLQSVREGCRTR
SQCRHSGPLRVAMKFPARSTRGATNKKAESRQPSENSVTDSNSDSEDESGMNFLEKRALNIKQNKAMLAK
LMSELESFPGSFRGRHPLPGSDSQSRRPRRRTFPGVASRRNPERRARPLTRSRSRILGSLDALPMEEEEE
EDKYMLVRKRKTVDGYMNEDDLPRSRRSRSSVTLPHIIRPVEEITEEELENVCSNSREKIYNRSLGSTCH
QCRQKTIDTKTNCRNPDCWGVRGQFCGPCLRNRYGEEVRDALLDPNWHCPPCRGICNCSFCRQRDGRCAT
GVLVYLAKYHGFGNVHAYLKSLKQEFEMQA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_031942
ORF Size 1350 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031942.5
RefSeq Size 2839 bp
RefSeq ORF 1353 bp
Locus ID 83879
UniProt ID Q9BWT1
Cytogenetics 2q31.1
MW 51.3 kDa
Summary This gene was identified as a c-Myc responsive gene, and behaves as a direct c-Myc target gene. Overexpression of this gene is found to enhance the transformation of lymphoblastoid cells, and it complements a transformation-defective Myc Box II mutant, suggesting its involvement in c-Myc-mediated cell transformation. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. provided by RefSeq, Jul 2008
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"NM_031942" in other vectors (6)
SKU Description Size Price
RC216735L1 Lenti ORF clone of Human cell division cycle associated 7 (CDCA7), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC216735L2 Lenti ORF clone of Human cell division cycle associated 7 (CDCA7), transcript variant 1, mGFP tagged 10 ug
$757.00
RC216735L3 Lenti ORF clone of Human cell division cycle associated 7 (CDCA7), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC216735L4 Lenti ORF clone of Human cell division cycle associated 7 (CDCA7), transcript variant 1, mGFP tagged 10 ug
$757.00
RG216735 CDCA7 (tGFP-tagged) - Human cell division cycle associated 7 (CDCA7), transcript variant 1 10 ug
$657.00
SC311382 CDCA7 (untagged)-Human cell division cycle associated 7 (CDCA7), transcript variant 1 10 ug
$457.00

Plasmid Map for RC216735

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC216735
Circular map for RC216735

file-alt Citations

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