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SIM2 (NM_009586) Human Tagged ORF Clone

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SKU
RC216257
SIM2 (Myc-DDK-tagged)-Human single-minded homolog 2 (Drosophila) (SIM2), transcript variant SIM2s
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $531.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SIM2
Synonyms bHLHe15; HMC13F06; HMC29C01; SIM
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216257 representing NM_009586
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGAGAAGTCCAAGAATGCGGCCAAGACCAGGAGGGAGAAGGAAAATGGCGAGTTTTACGAGCTTG
CCAAGCTGCTCCCGCTGCCGTCGGCCATCACTTCGCAGCTGGACAAAGCGTCCATCATCCGCCTCACCAC
GAGCTACCTGAAGATGCGCGCCGTCTTCCCCGAAGGTTTAGGAGACGCGTGGGGACAGCCGAGCCGCGCC
GGGCCCCTGGACGGCGTCGCCAAGGAGCTGGGATCGCACTTGCTGCAGACTTTGGATGGATTTGTTTTTG
TGGTAGCATCTGATGGCAAAATCATGTATATATCCGAGACCGCTTCTGTCCATTTAGGCTTATCCCAGGT
GGAGCTCACGGGCAACAGTATTTATGAATACATCCATCCTTCTGACCACGATGAGATGACCGCTGTCCTC
ACGGCCCACCAGCCGCTGCACCACCACCTGCTCCAAGAGTATGAGATAGAGAGGTCGTTCTTTCTTCGAA
TGAAATGTGTCTTGGCGAAAAGGAACGCGGGCCTGACCTGCAGCGGATACAAGGTCATCCACTGCAGTGG
CTACTTGAAGATCAGGCAGTATATGCTGGACATGTCCCTGTACGACTCCTGCTACCAGATTGTGGGGCTG
GTGGCCGTGGGCCAGTCGCTGCCACCCAGTGCCATCACCGAGATCAAGCTGTACAGTAACATGTTCATGT
TCAGGGCCAGCCTTGACCTGAAGCTGATATTCCTGGATTCCAGGGTGACCGAGGTGACGGGGTACGAGCC
GCAGGACCTGATCGAGAAGACCCTATACCATCACGTGCACGGCTGCGACGTGTTCCACCTCCGCTACGCA
CACCACCTCCTGTTGGTGAAGGGCCAGGTCACCACCAAGTACTACCGGCTGCTGTCCAAGCGGGGCGGCT
GGGTGTGGGTGCAGAGCTACGCCACCGTGGTGCACAACAGCCGCTCGTCCCGGCCCCACTGCATCGTGAG
TGTCAATTATGTACTCACGGAGATTGAATACAAGGAACTTCAGCTGTCCCTGGAGCAGGTGTCCACTGCC
AAGTCCCAGGACTCCTGGAGGACCGCCTTGTCTACCTCACAAGAAACTAGGAAATTAGTGAAACCCAAAA
ATACCAAGATGAAGACAAAGCTGAGAACAAACCCTTACCCCCCACAGCAATACAGCTCGTTCCAAATGGA
CAAACTGGAATGCGGCCAGCTCGGAAACTGGAGAGCCAGTCCCCCTGCAAGCGCTGCTGCTCCTCCAGAA
CTGCAGCCCCACTCAGAAAGCAGTGACCTTCTGTACACGCCATCCTACAGCCTGCCCTTCTCCTACCATT
ACGGACACTTCCCTCTGGACTCTCACGTCTTCAGCAGCAAAAAGCCAATGTTGCCGGCCAAGTTCGGGCA
GCCCCAAGGATCCCCTTGTGAGGTGGCACGCTTTTTCCTGAGCACACTGCCAGCCAGCGGTGAATGCCAG
TGGCATTATGCCAACCCCCTAGTGCCTAGCAGCTCGTCTCCAGCTAAAAATCCTCCAGAGCCACCGGCGA
ACACTGCTAGGCACAGCCTGGTGCCAAGCTACGAAGGTGGGTCAGGTCTGCTCGTGGGGAAGGTGGGAGG
ACTGCGCACGGCCGGGAGCCGAAGCAGCCATGGCGGTGGGTGGCAGATGGAGACAGAACCCTCACGCTTT
GGGCAAACTTGCCCTCTTTCTGCTTCTAAG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC216257 representing NM_009586
Red=Cloning site Green=Tags(s)

MKEKSKNAAKTRREKENGEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRAVFPEGLGDAWGQPSRA
GPLDGVAKELGSHLLQTLDGFVFVVASDGKIMYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVL
TAHQPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGL
VAVGQSLPPSAITEIKLYSNMFMFRASLDLKLIFLDSRVTEVTGYEPQDLIEKTLYHHVHGCDVFHLRYA
HHLLLVKGQVTTKYYRLLSKRGGWVWVQSYATVVHNSRSSRPHCIVSVNYVLTEIEYKELQLSLEQVSTA
KSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPE
LQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTLPASGECQ
WHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYEGGSGLLVGKVGGLRTAGSRSSHGGGWQMETEPSRF
GQTCPLSASK

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_009586
ORF Size 1710 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009586.5
RefSeq Size 2823 bp
RefSeq ORF 1713 bp
Locus ID 6493
UniProt ID Q14190
Cytogenetics 21q22.13
Protein Families Druggable Genome, Transcription Factors
MW 63.3 kDa
Summary This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants. provided by RefSeq, Sep 2014
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Documents
Resources
"NM_009586" in other vectors (6)
SKU Description Size Price
RC216257L1 Lenti ORF clone of Human single-minded homolog 2 (Drosophila) (SIM2), transcript variant SIM2s, Myc-DDK-tagged 10 ug
$831.00
RC216257L2 Lenti ORF clone of Human single-minded homolog 2 (Drosophila) (SIM2), transcript variant SIM2s, mGFP tagged 10 ug
$831.00
RC216257L3 Lenti ORF clone of Human single-minded homolog 2 (Drosophila) (SIM2), transcript variant SIM2s, Myc-DDK-tagged 10 ug
$831.00
RC216257L4 Lenti ORF clone of Human single-minded homolog 2 (Drosophila) (SIM2), transcript variant SIM2s, mGFP tagged 10 ug
$831.00
RG216257 SIM2 (tGFP-tagged) - Human single-minded homolog 2 (Drosophila) (SIM2), transcript variant SIM2s 10 ug
$489.00 $731.00
SC303922 SIM2 (untagged)-Human single-minded homolog 2 (Drosophila) (SIM2), transcript variant SIM2s 10 ug
$532.00

Plasmid Map for RC216257

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC216257
Circular map for RC216257

file-alt Citations

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