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PTPN7 (NM_080588) Human Tagged ORF Clone

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SKU
RC216254
PTPN7 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 7 (PTPN7), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "PTPN7 or NM_080588"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PTPN7
Synonyms BPTP-4; HEPTP; LC-PTP; LPTP; PTPNI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216254 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGCCTCCTTCTGGCCAATCAGGCAGGCCAGAGAACAGCAGCGGAGGGCCCTGTCTTTCAGGCAGA
CCTCATGGCTGAGTGAGCCTCCCCTGGGCCCAGCACCCCACCTCAGCATGGTCCAAGCCCATGGGGGGCG
CTCCAGAGCACAGCCGTTGACCTTGTCTTTGGGGGCAGCCATGACCCAGCCTCCGCCTGAAAAAACGCCA
GCCAAGAAGCATGTGCGACTGCAGGAGAGGCGGGGCTCCAATGTGGCTCTGATGCTGGACGTTCGGTCCC
TGGGGGCCGTAGAACCCATCTGCTCTGTGAACACACCCCGGGAGGTCACCCTACACTTTCTGCGCACTGC
TGGACACCCCCTTACCCGCTGGGCCCTTCAGCGCCAGCCACCCAGCCCCAAGCAACTGGAAGAAGAATTC
TTGAAGATCCCTTCAAACTTTGTCAGCCCCGAAGACCTGGACATCCCTGGCCACGCCTCCAAGGACCGAT
ACAAGACCATCTTGCCAAATCCCCAGAGCCGTGTCTGTCTAGGCCGGGCACAGAGCCAGGAGGACGGAGA
TTACATCAATGCCAACTACATCCGAGGCTATGACGGGAAGGAGAAGGTCTACATTGCCACCCAGGGCCCC
ATGCCCAACACTGTGTCGGACTTCTGGGAGATGGTGTGGCAAGAGGAAGTGTCCCTCATTGTCATGCTCA
CTCAGCTCCGAGAGGGCAAGGAGAAATGTGTCCACTACTGGCCCACAGAAGAGGAAACCTATGGACCCTT
CCAGATCCGCATCCAGGACATGAAAGAGTGCCCAGAATACACTGTGCGGCAGCTCACCATCCAGTACCAG
GAAGAGCGCCGGTCAGTAAAGCACATCCTCTTTTCGGCCTGGCCAGACCATCAGACACCAGAATCAGCTG
GGCCCCTGCTGCGCCTAGTGGCAGAGGTGGAGGAGAGCCCGGAGACAGCCGCCCACCCCGGGCCTATCGT
AGTCCACTGCAGTGCAGGGATTGGCCGGACGGGCTGCTTCATCGCCACGCGAATTGGCTGTCAACAGCTG
AAAGCCCGAGGAGAAGTGGACATTCTGGGTATTGTGTGCCAACTGCGGCTAGACAGAGGGGGGATGATCC
AGACGGCAGAGCAGTACCAGTTCCTGCACCACACTTTGGCCCTGTATGCAGGCCAGCTGCCTGAGGAACC
CAGCCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC216254 protein sequence
Red=Cloning site Green=Tags(s)

MGASFWPIRQAREQQRRALSFRQTSWLSEPPLGPAPHLSMVQAHGGRSRAQPLTLSLGAAMTQPPPEKTP
AKKHVRLQERRGSNVALMLDVRSLGAVEPICSVNTPREVTLHFLRTAGHPLTRWALQRQPPSPKQLEEEF
LKIPSNFVSPEDLDIPGHASKDRYKTILPNPQSRVCLGRAQSQEDGDYINANYIRGYDGKEKVYIATQGP
MPNTVSDFWEMVWQEEVSLIVMLTQLREGKEKCVHYWPTEEETYGPFQIRIQDMKECPEYTVRQLTIQYQ
EERRSVKHILFSAWPDHQTPESAGPLLRLVAEVEESPETAAHPGPIVVHCSAGIGRTGCFIATRIGCQQL
KARGEVDILGIVCQLRLDRGGMIQTAEQYQFLHHTLALYAGQLPEEPSP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080588
ORF Size 1197 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080588.2
RefSeq Size 3265 bp
RefSeq ORF 1200 bp
Locus ID 5778
UniProt ID P35236, A0A024R9A7, P35236-2
Cytogenetics 1q32.1
Protein Families Druggable Genome, Phosphatase
Protein Pathways MAPK signaling pathway
MW 45 kDa
Summary The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This gene is preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells. The non-catalytic N-terminus of this PTP can interact with MAP kinases and suppress the MAP kinase activities. This PTP was shown to be involved in the regulation of T cell antigen receptor (TCR) signaling, which was thought to function through dephosphorylating the molecules related to MAP kinase pathway. Multiple alternatively spliced transcript variants have been found for this gene. provided by RefSeq, Dec 2010
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"NM_080588" in other vectors (4)
SKU Description Size Price
RC216254L3 Lenti ORF clone of Human protein tyrosine phosphatase, non-receptor type 7 (PTPN7), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC216254L4 Lenti ORF clone of Human protein tyrosine phosphatase, non-receptor type 7 (PTPN7), transcript variant 2, mGFP tagged 10 ug
$757.00
RG216254 PTPN7 (tGFP-tagged) - Human protein tyrosine phosphatase, non-receptor type 7 (PTPN7), transcript variant 2 10 ug
$489.00 $657.00
SC128004 PTPN7 (untagged)-Human protein tyrosine phosphatase, non-receptor type 7 (PTPN7), transcript variant 2 10 ug
$457.00

Plasmid Map for RC216254

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC216254
Circular map for RC216254

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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