PTPN7 (NM_080588) Human Tagged ORF Clone

SKU RG216254

PTPN7 (tGFP-tagged) - Human protein tyrosine phosphatase, non-receptor type 7 (PTPN7), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PTPN7
• Synonyms: BPTP-4; HEPTP; LC-PTP; LPTP; PTPNI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216254 representing NM_080588; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGAGCCTCCTTCTGGCCAATCAGGCAGGCCAGAGAACAGCAGCGGAGGGCCCTGTCTTTCAGGCAGA; CCTCATGGCTGAGTGAGCCTCCCCTGGGCCCAGCACCCCACCTCAGCATGGTCCAAGCCCATGGGGGGCG; CTCCAGAGCACAGCCGTTGACCTTGTCTTTGGGGGCAGCCATGACCCAGCCTCCGCCTGAAAAAACGCCA; GCCAAGAAGCATGTGCGACTGCAGGAGAGGCGGGGCTCCAATGTGGCTCTGATGCTGGACGTTCGGTCCC; TGGGGGCCGTAGAACCCATCTGCTCTGTGAACACACCCCGGGAGGTCACCCTACACTTTCTGCGCACTGC; TGGACACCCCCTTACCCGCTGGGCCCTTCAGCGCCAGCCACCCAGCCCCAAGCAACTGGAAGAAGAATTC; TTGAAGATCCCTTCAAACTTTGTCAGCCCCGAAGACCTGGACATCCCTGGCCACGCCTCCAAGGACCGAT; ACAAGACCATCTTGCCAAATCCCCAGAGCCGTGTCTGTCTAGGCCGGGCACAGAGCCAGGAGGACGGAGA; TTACATCAATGCCAACTACATCCGAGGCTATGACGGGAAGGAGAAGGTCTACATTGCCACCCAGGGCCCC; ATGCCCAACACTGTGTCGGACTTCTGGGAGATGGTGTGGCAAGAGGAAGTGTCCCTCATTGTCATGCTCA; CTCAGCTCCGAGAGGGCAAGGAGAAATGTGTCCACTACTGGCCCACAGAAGAGGAAACCTATGGACCCTT; CCAGATCCGCATCCAGGACATGAAAGAGTGCCCAGAATACACTGTGCGGCAGCTCACCATCCAGTACCAG; GAAGAGCGCCGGTCAGTAAAGCACATCCTCTTTTCGGCCTGGCCAGACCATCAGACACCAGAATCAGCTG; GGCCCCTGCTGCGCCTAGTGGCAGAGGTGGAGGAGAGCCCGGAGACAGCCGCCCACCCCGGGCCTATCGT; AGTCCACTGCAGTGCAGGGATTGGCCGGACGGGCTGCTTCATCGCCACGCGAATTGGCTGTCAACAGCTG; AAAGCCCGAGGAGAAGTGGACATTCTGGGTATTGTGTGCCAACTGCGGCTAGACAGAGGGGGGATGATCC; AGACGGCAGAGCAGTACCAGTTCCTGCACCACACTTTGGCCCTGTATGCAGGCCAGCTGCCTGAGGAACC; CAGCCCC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216254 representing NM_080588; Red=Cloning site Green=Tags(s); ; MGASFWPIRQAREQQRRALSFRQTSWLSEPPLGPAPHLSMVQAHGGRSRAQPLTLSLGAAMTQPPPEKTP; AKKHVRLQERRGSNVALMLDVRSLGAVEPICSVNTPREVTLHFLRTAGHPLTRWALQRQPPSPKQLEEEF; LKIPSNFVSPEDLDIPGHASKDRYKTILPNPQSRVCLGRAQSQEDGDYINANYIRGYDGKEKVYIATQGP; MPNTVSDFWEMVWQEEVSLIVMLTQLREGKEKCVHYWPTEEETYGPFQIRIQDMKECPEYTVRQLTIQYQ; EERRSVKHILFSAWPDHQTPESAGPLLRLVAEVEESPETAAHPGPIVVHCSAGIGRTGCFIATRIGCQQL; KARGEVDILGIVCQLRLDRGGMIQTAEQYQFLHHTLALYAGQLPEEPSP; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080588
• ORF Size: 1197 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_080588.2
• RefSeq Size: 3263 bp
• RefSeq ORF: 1200 bp
• Locus ID: 5778
• UniProt ID: P35236
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: MAPK signaling pathway
• Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This gene is preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells. The non-catalytic N-terminus of this PTP can interact with MAP kinases and suppress the MAP kinase activities. This PTP was shown to be involved in the regulation of T cell antigen receptor (TCR) signaling, which was thought to function through dephosphorylating the molecules related to MAP kinase pathway. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2010]