TBC1D8B (NM_017752) Human Tagged ORF Clone

  • TrueORF®
SKU
RC215906
TBC1D8B (Myc-DDK-tagged)-Human TBC1 domain family, member 8B (with GRAM domain) (TBC1D8B), transcript variant 1
  $1,462.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TBC1D8B
Synonyms GRAMD8B; NPHS20
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017752
ORF Size 3360 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017752.2, NP_060222.2
RefSeq Size 5726 bp
RefSeq ORF 3363 bp
Locus ID 54885
UniProt ID Q0IIM8
Cytogenetics Xq22.3
MW 128.5 kDa
Summary This gene encodes a protein with a TBC (Tre-2/Bub2/CDC16) domain. Some mammalian proteins with this domain have been shown to function as Rab-GAPs by binding to specific Rab proteins and affecting their GTPase activity. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2011
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Resources
"NM_017752" in other vectors (4)
SKU Description Size Price
RC215906L3 Lenti-ORF clone of TBC1D8B (Myc-DDK-tagged)-Human TBC1 domain family, member 8B (with GRAM domain) (TBC1D8B), transcript variant 1 10 ug
$1,762.00
RC215906L4 Lenti-ORF clone of TBC1D8B (mGFP-tagged)-Human TBC1 domain family, member 8B (with GRAM domain) (TBC1D8B), transcript variant 1 10 ug
$1,762.00
RG215906 TBC1D8B (tGFP-tagged) - Human TBC1 domain family, member 8B (with GRAM domain) (TBC1D8B), transcript variant 1 10 ug
$1,662.00
SC304523 TBC1D8B (untagged)-Human TBC1 domain family, member 8B (with GRAM domain) (TBC1D8B), transcript variant 1 10 ug
$1,073.00

Plasmid Map for RC215906

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Cloning scheme for RC215906

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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