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EBF4 (NM_020833) Human Tagged ORF Clone

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SKU
RC215467
EBF4 (Myc-DDK-tagged)-Human early B-cell factor 4 (EBF4)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $503.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EBF4
Synonyms COE4; early B-cell factor 4; KIAA1442
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215467 representing NM_020833
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGCTCTGCCCCGCAGCGGGCTGAACCTGAAGGAGGAGCCGCTGCTGCCCGCCGGCCTGGGCTCAG
TGCGCTCCTGGATGCAGGGCGCGGGCATCCTGGACGCCAGCACCGCGGCGCAGAGTGGCGTGGGTCTGGC
ACGAGCACATTTTGAGAAGCAGCCTCCCTCCAACCTCAGGAAATCCAACTTCTTCCACTTCGTGCTGGCC
ATGTACGACCGGCAGGGGCAGCCCGTGGAGGTGGAGCGCACAGCCTTCATCGACTTCGTGGAAAAGGACC
GAGAGCCCGGGGCGGAAAAGACTAACAATGGGATCCATTACCGCCTCCGGCTGGTGTATAACAATGGACT
GCGGACAGAGCAAGACCTCTACGTGCGTCTCATCGACTCCATGTCCAAACAGGCCATCATCTATGAGGGG
CAGGACAAGAACCCCGAAATGTGCCGAGTGCTGCTCACCCATGAGATCATGTGCAGCCGGTGCTGTGACC
GGAAGAGCTGTGGCAACCGGAATGAGACGCCCTCAGACCCCGTCATCATTGACAGGTTCTTCCTCAAGTT
CTTCCTCAAATGCAACCAGAACTGCCTGAAGAATGCGGGGAATCCCAGAGACATGCGCCGCTTCCAGGTG
GTGGTGTCCACGACGGTGAGCGTGGACGGACACGTGCTGGCCGTGTCCGACAACATGTTTGTGCACAACA
ACTCCAAGCATGGCCGCAGGGCGCGCCGCCTGGACCCCTCCGAAGCTGCCACCCCCTGCATCAAGGCCAT
CAGCCCCGGGGAGGGCTGGACCACGGGCGGCGCCACCGTCATTGTCATCGGCGACAACTTCTTCGACGGG
TTGCAGGTCGTGTTCGGAAACGTGCTCGTGTGGAGCGAGCTCATCACGCCCCACGCCATCCGGGTGCAGA
CGCCCCCGCGGCACATCCCCGGGGTGGTGGAGGTGACCCTCTCCTACAAGTCCAAGCAGTTTTGCAAGGG
ATGCCCCGGCCGCTTTGTCTACACAGCTCTGAACGAGCCCACCATTGACTACGGATTCCAGAGGCTACAG
AAAGTCATTCCCAGACACCCCGGAGACCCCGAGAGGCTGCCCAAGGAAGTGCTGCTGAAGCGGGCGGCCG
ACTTGGCAGAAGCCCTGTACGGAGTGCCCGGCAGTAACCAGGAGCTGCTCCTGAAGCGCGCGGCGGACGT
GGCCGAGGCTCTGTACAGCACCCCCCGCGCACCCGGGCCGCTCGCACCCCTGGCCCCGAGCCACCCACAC
CCCGCCGTCGTGGGCATCAACGCCTTCAGCAGCCCGCTGGCCATCGCCGTCGGGGACGCCACCCCGGGGC
CCGAGCCGGGCTACGCGCGCAGCTGCAGCAGCGCGTCCCCCCGCGGGTTCGCGCCCAGCCCCGGCTCGCA
GCAGAGCGGCTACGGCGGCGGCCTCGGAGCTGGCCTGGGCGGCTACGGCGCGCCGGGCGTGGCCGGCCTC
GGCGTGCCTGGGTCCCCCAGCTTCCTCAATGGCTCCACCGCCACCTCGCCCTTCGCCAAGGAGCGCCTTC
GCCCCCGTGCTGCGCCCCCCAAGCTCCCCACCCCAGGCCTGCCCCAGAGCCCACGGAGAGGGGCTTCCAG
ACCAGTCTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC215467 representing NM_020833
Red=Cloning site Green=Tags(s)

MDALPRSGLNLKEEPLLPAGLGSVRSWMQGAGILDASTAAQSGVGLARAHFEKQPPSNLRKSNFFHFVLA
MYDRQGQPVEVERTAFIDFVEKDREPGAEKTNNGIHYRLRLVYNNGLRTEQDLYVRLIDSMSKQAIIYEG
QDKNPEMCRVLLTHEIMCSRCCDRKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQV
VVSTTVSVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEAATPCIKAISPGEGWTTGGATVIVIGDNFFDG
LQVVFGNVLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGCPGRFVYTALNEPTIDYGFQRLQ
KVIPRHPGDPERLPKEVLLKRAADLAEALYGVPGSNQELLLKRAADVAEALYSTPRAPGPLAPLAPSHPH
PAVVGINAFSSPLAIAVGDATPGPEPGYARSCSSASPRGFAPSPGSQQSGYGGGLGAGLGGYGAPGVAGL
GVPGSPSFLNGSTATSPFAKERLRPRAAPPKLPTPGLPQSPRRGASRPVF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020833
ORF Size 1620 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020833.2
RefSeq Size 2814 bp
RefSeq ORF 1622 bp
Locus ID 57593
Cytogenetics 20p13
MW 58.7 kDa
Summary EBF4 belongs to the conserved Olf/EBF family of helix-loop-helix transcription factors, members of which play important roles in neural development and B-cell maturation (Wang et al., 2002 PubMed 12139918).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_020833" in other vectors (3)
SKU Description Size Price
RC215467L3 Lenti ORF clone of Human early B-cell factor 4 (EBF4), Myc-DDK-tagged 10 ug
$803.00
RC215467L4 Lenti ORF clone of Human early B-cell factor 4 (EBF4), mGFP tagged 10 ug
$803.00
RG215467 EBF4 (tGFP-tagged) - Human early B-cell factor 4 (EBF4) 10 ug
$703.00

Plasmid Map for RC215467

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC215467
Circular map for RC215467

file-alt Citations

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