KIRREL2 (NM_032123) Human Tagged ORF Clone
SKU
RC215296
KIRREL2 (Myc-DDK-tagged)-Human kin of IRRE like 2 (Drosophila) (KIRREL2), transcript variant 1
$589.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | KIRREL2 |
| Synonyms | FILTRIN; NEPH3; NLG1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_032123 |
| ORF Size | 1899 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_032123.7 |
| RefSeq Size | 2153 bp |
| RefSeq ORF | 1902 bp |
| Locus ID | 84063 |
| UniProt ID | Q6UWL6 |
| Cytogenetics | 19q13.12 |
| Protein Families | Transmembrane |
| MW | 66.9 kDa |
| Summary | This gene encodes a type I transmembrane protein and member of the immunoglobulin superfamily of cell adhesion molecules. The encoded protein localizes to adherens junctions in pancreatic beta cells and regulates insulin secretion. Autoantibodies against the encoded protein have been detected in serum from patients with type 1 diabetes. This gene may also play a role in glomerular development and decreased expression of this gene has been observed in human glomerular diseases. This gene and the related opposite-strand gene nephrin (GeneID: 527362) are regulated by a bidirectional promoter. provided by RefSeq, Jul 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_032123" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC215296L1 | Lenti ORF clone of Human kin of IRRE like 2 (Drosophila) (KIRREL2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$889.00
|
|
| RC215296L2 | Lenti ORF clone of Human kin of IRRE like 2 (Drosophila) (KIRREL2), transcript variant 1, mGFP tagged | 10 ug |
$889.00
|
|
| RC215296L3 | Lenti ORF clone of Human kin of IRRE like 2 (Drosophila) (KIRREL2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$889.00
|
|
| RC215296L4 | Lenti ORF clone of Human kin of IRRE like 2 (Drosophila) (KIRREL2), transcript variant 1, mGFP tagged | 10 ug |
$889.00
|
|
| RG215296 | KIRREL2 (tGFP-tagged) - Human kin of IRRE like 2 (Drosophila) (KIRREL2), transcript variant 1 | 10 ug |
$489.00
$789.00
|
|
| SC305401 | KIRREL2 (untagged)-Human kin of IRRE like 2 (Drosophila) (KIRREL2), transcript variant 1 | 10 ug |
$649.00
|
|
Citations
A functional variant in NEPH3 gene confers high risk of renal failure in primary hematuric glomerulopathies. Evidence for predisposition to microalbuminuria in the general population
Voskarides, Stefanou, Pieri et al
PLoS ONE (2017) 12 (3), e0174274 DOI: 10.1371/journal.pone.0174274
Voskarides, Stefanou, Pieri et al
PLoS ONE (2017) 12 (3), e0174274 DOI: 10.1371/journal.pone.0174274
AI Summary: OriGene contributed to this study by providing the cloned human NEPH3 (filtrin) cDNA in a pCMV6 eukaryotic expression vector with a FLAG-tag, which was used to investigate the functional impact of NEPH3 variants associated with renal failure risk.
Snippet: Cloned human NEPH3 (filtrin) cDNA into a pCMV6 eukaryotic expression vector with available FLAG-tag sequence was purchased from Origene (Rockville, USA). NEPH3 353V (GTG triplet) was mutated to 353M (ATG triplet) via the Quick Change Site-Directed Mutagenesis kit (Stratagene, La Jolla, CA, USA)
Showing 1-1 of 1 papers.
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