OLFM4 (NM_006418) Human Tagged ORF Clone
$289.00
$475.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | OLFM4 |
| Synonyms | bA209J19.1; GC1; GW112; hGC-1; hOLfD; OlfD; OLM4; UNQ362 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_006418 |
| ORF Size | 1530 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_006418.5 |
| RefSeq Size | 2844 bp |
| RefSeq ORF | 1533 bp |
| Locus ID | 10562 |
| UniProt ID | Q6UX06 |
| Cytogenetics | 13q14.3 |
| Domains | OLF |
| Protein Families | Druggable Genome |
| MW | 57.28 kDa |
| Summary | This gene was originally cloned from human myeloblasts and found to be selectively expressed in inflammed colonic epithelium. This gene encodes a member of the olfactomedin family. The encoded protein is an antiapoptotic factor that promotes tumor growth and is an extracellular matrix glycoprotein that facilitates cell adhesion. provided by RefSeq, Mar 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_006418" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC214942L1 | Lenti ORF clone of Human olfactomedin 4 (OLFM4), Myc-DDK-tagged | 10 ug |
$775.00
|
|
| RC214942L2 | Lenti ORF clone of Human olfactomedin 4 (OLFM4), mGFP tagged | 10 ug |
$775.00
|
|
| RC214942L3 | Lenti ORF clone of Human olfactomedin 4 (OLFM4), Myc-DDK-tagged | 10 ug |
$775.00
|
|
| RC214942L4 | Lenti ORF clone of Human olfactomedin 4 (OLFM4), mGFP tagged | 10 ug |
$775.00
|
|
| RG214942 | OLFM4 (tGFP-tagged) - Human olfactomedin 4 (OLFM4) | 10 ug |
$489.00
$675.00
|
|
| SC108914 | OLFM4 (untagged)-Human olfactomedin 4 (OLFM4) | 10 ug |
$476.00
|
|
Citations
Notch and TNF-α signaling promote cytoplasmic accumulation of OLFM4 in intestinal epithelium cells and exhibit a cell protective role in the inflamed mucosa of IBD patients
Kuno, Ito, Kawamoto et al
Biochem Biophys Rep (2021) 25, 100906 DOI: 10.1016/j.bbrep.2020.100906
Kuno, Ito, Kawamoto et al
Biochem Biophys Rep (2021) 25, 100906 DOI: 10.1016/j.bbrep.2020.100906
Cited by 21
AI Summary: OriGene contributed to this study by providing the OLFM4 expression vector (RC214942), which was used to investigate the regulation and function of OLFM4 in human intestinal epithelial cells.
Snippet: L4.10[luc2] vector (Promega), between the KpnI and NheI sites. The nucleotide position of the translation start site was defined as the +1 position. The expression vector for OLFM4 was purchased from ORIGENE (RC214942). 2.10 Data presentation Unless otherwise indicated, quantitative data are displayed as mean ± SEM from at least three independent experiments. Significance was assessed using an
Showing 1-1 of 1 papers.
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