OLFM4 (NM_006418) Human Tagged ORF Clone
$489.00
$675.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | OLFM4 |
| Synonyms | bA209J19.1; GC1; GW112; hGC-1; hOLfD; OlfD; OLM4; UNQ362 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_006418 |
| ORF Size | 1530 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_006418.5 |
| RefSeq Size | 2844 bp |
| RefSeq ORF | 1533 bp |
| Locus ID | 10562 |
| UniProt ID | Q6UX06 |
| Cytogenetics | 13q14.3 |
| Domains | OLF |
| Protein Families | Druggable Genome |
| Summary | This gene was originally cloned from human myeloblasts and found to be selectively expressed in inflammed colonic epithelium. This gene encodes a member of the olfactomedin family. The encoded protein is an antiapoptotic factor that promotes tumor growth and is an extracellular matrix glycoprotein that facilitates cell adhesion. provided by RefSeq, Mar 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_006418" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC214942 | OLFM4 (Myc-DDK-tagged)-Human olfactomedin 4 (OLFM4) | 10 ug |
$289.00
$475.00
|
|
| RC214942L1 | Lenti ORF clone of Human olfactomedin 4 (OLFM4), Myc-DDK-tagged | 10 ug |
$775.00
|
|
| RC214942L2 | Lenti ORF clone of Human olfactomedin 4 (OLFM4), mGFP tagged | 10 ug |
$775.00
|
|
| RC214942L3 | Lenti ORF clone of Human olfactomedin 4 (OLFM4), Myc-DDK-tagged | 10 ug |
$775.00
|
|
| RC214942L4 | Lenti ORF clone of Human olfactomedin 4 (OLFM4), mGFP tagged | 10 ug |
$775.00
|
|
| SC108914 | OLFM4 (untagged)-Human olfactomedin 4 (OLFM4) | 10 ug |
$476.00
|
|
Citations
High expression of olfactomedin-4 is correlated with chemoresistance and poor prognosis in pancreatic cancer
Ohkuma, Yada, Ishikawa et al
PLoS ONE (2020) 15 (1), e0226707 DOI: 10.1371/journal.pone.0226707
Ohkuma, Yada, Ishikawa et al
PLoS ONE (2020) 15 (1), e0226707 DOI: 10.1371/journal.pone.0226707
Cited by 29
AI Summary: OriGene’s pCMV6-Ac-OLFM4-GFP tag plasmid (NM_006418) was used in this study to induce OLFM4 overexpression in pancreatic cancer cell lines, enabling the investigation of OLFM4’s role in chemoresistance and prognosis.
Snippet: … OLFM4 expression plasmid (pCMV6-Ac-OLFM4-GFP tag plasmids, NM_006418) was purchased from OriGene Technologies, Inc. … OLFM4 specific siRNA (OLFM4 Silencer Select siRNA, Cat# 4392420) and negative control siRNA (Silencer Select;;... … OLFM4 expression plasmid (pCMV6-Ac-OLFM4-GFP tag plasmids, NM_006418) was purchased from OriGene Technologies, Inc. … OLFM4 specific siRNA (OLFM4 Silencer Select siRNA, Cat# 4392420) and negative control siRNA (Silencer Select
Olfactomedin 4 deficiency promotes prostate neoplastic progression and is associated with upregulation of the hedgehog-signaling pathway
Li, Liu, Chen et al
Sci Rep (2015) 5, 16974 DOI: 10.1038/srep16974
Li, Liu, Chen et al
Sci Rep (2015) 5, 16974 DOI: 10.1038/srep16974
Cited by 21
AI Summary: OriGene products, specifically the pCMV-6-AC-GFP tag vector and the pCMV-6-OLFM4-GFP tag plasmid, were used in this study to generate prostate cancer cells stably expressing OLFM4, enabling the researchers to investigate OLFM4's role in regulating hedgehog signaling and prostate cancer progression.
Snippet: fetal bovine serum (FBS) until 90% confluent 16 ,28 . The pCMV-6-AC-GFP tag-vector and pCMV-6-OLFM4-GFP tag plasmids were purchased from Origene. Stably expressing OLFM4-green fluorescent protein (GFP) tag-expressing ... Cite SaveSaving
Showing 1-2 of 2 papers.
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