L Kynurenine Hydrolase (KYNU) (NM_003937) Human Tagged ORF Clone
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | L Kynurenine Hydrolase |
| Synonyms | KYNUU; VCRL2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003937 |
| ORF Size | 1395 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003937.3 |
| RefSeq Size | 1688 bp |
| RefSeq ORF | 1398 bp |
| Locus ID | 8942 |
| UniProt ID | Q16719 |
| Cytogenetics | 2q22.2 |
| Protein Families | Protease |
| Protein Pathways | Metabolic pathways, Tryptophan metabolism |
| MW | 52.2 kDa |
| Summary | Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Alternative splicing results in multiple transcript variants. provided by RefSeq, Nov 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003937" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC214932L1 | Lenti ORF clone of Human kynureninase (KYNU), transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC214932L2 | Lenti ORF clone of Human kynureninase (KYNU), transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RC214932L3 | Lenti ORF clone of Human kynureninase (KYNU), transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC214932L4 | Lenti ORF clone of Human kynureninase (KYNU), transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RG214932 | KYNU (tGFP-tagged) - Human kynureninase (KYNU), transcript variant 1 | 10 ug |
$489.00
$886.00
|
|
| SC317002 | KYNU (untagged)-Human kynureninase (KYNU), transcript variant 1 | 10 ug |
$457.00
|
|
Citations
Differential Gene Expression and Methylation Analysis of Melanoma in TCGA Database to Further Study the Expression Pattern of KYNU in Melanoma
Wang, Liu, Huang et al
J Pers Med (2022) 12 (8) DOI: 10.3390/jpm12081209
Wang, Liu, Huang et al
J Pers Med (2022) 12 (8) DOI: 10.3390/jpm12081209
Cited by 9
AI Summary: In this study, the OriGene kynuORF-Pcmv66-Entry recombinant plasmid (#RC214932) was used to overexpress KYNU in melanoma and keratinocyte cell lines, enabling the authors to investigate KYNU's role in melanoma cell proliferation, apoptosis, invasion, and migration.
Snippet: medium (H-DMEM) containing 10% fetal bovine serum and 1% penicillin/streptomycin, before incubating at 37 °C with 5% CO2. For KYNU overexpression, 1 μg of kynuORF-Pcmv66-Entry recombinant plasmid (OriGene, #RC214932) was mixed with 3 μL of PEI transfection reagent, and incubated with opti-MEM for 20 min for cell transfection. When the cells grew well and the cell fusion rate reached 50–60%,
New cases that expand the genotypic and phenotypic spectrum of Congenital NAD Deficiency Disorder
Szot, Slavotinek, Chong et al
Hum Mutat (2021)
Szot, Slavotinek, Chong et al
Hum Mutat (2021)
Cited by 31
AI Summary: OriGene’s Human KYNU cDNA (product RC214932) was used as the template to clone and study disease-associated KYNU variants, enabling functional analysis of their impact on NAD synthesis in this research.
Snippet: pAG416GPD (Addgene plasmid #14148) by Gateway LR reaction. Human KYNU cDNA was amplified from Origene:RC214932 using cloning primers (Supp. Table S1), then gateway cloned into pDONR201 via BP reaction (Thermo Fisher) according
A novel role of kynureninase in the growth control of breast cancer cells and its relationships with breast cancer
Liu, Feng, Lai et al
J. Cell. Mol. Med. (2019) 23 (10), 6700-6707 DOI: 10.1111/jcmm.14547
Liu, Feng, Lai et al
J. Cell. Mol. Med. (2019) 23 (10), 6700-6707 DOI: 10.1111/jcmm.14547
AI Summary: OriGene’s pcDNA-KYNU plasmid (OriGene #RC214932) was used to overexpress kynureninase in breast cancer cells, enabling the study of its role in inhibiting cancer cell proliferation and tumor growth.
Snippet: 2.4 | Transfections The transfection of pcDNA‐KYNU (OriGene #RC214932) was performed by using the TransIT‐LT1 transfection reagent (Mirus) according to manufacturer's recommendations. Stable transfection was achieved by G418 selection
Showing 1-3 of 3 papers.
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