OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

PDE7A (NM_002603) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC214749
PDE7A (Myc-DDK-tagged)-Human phosphodiesterase 7A (PDE7A), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA338280 Rabbit Polyclonal Anti-PDE7A Antibody
100 ul
$585.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "PDE7A or NM_002603"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PDE7A
Synonyms HCP1; PDE7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214749 representing NM_002603
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAATTACATTGATCTGGTGTCTGGCCTTGGTTCTTATCAAGTGGATCACCTCTAAGAGGCGTGGAG
CTATTTCCTATGACAGTTCTGATCAGACTGCATTATACATTCGTATGCTAGGAGATGTACGTGTAAGGAG
CCGAGCAGGATTTGAATCAGAAAGAAGAGGTTCTCACCCATATATTGATTTTCGTATTTTCCACTCTCAA
TCTGAAATTGAAGTGTCTGTCTCTGCAAGGAATATCAGAAGGCTACTAAGTTTCCAGCGATATCTTAGAT
CTTCACGCTTTTTTCGTGGTACTGCGGTTTCAAATTCCCTAAACATTTTAGATGATGATTATAATGGACA
AGCCAAGTGTATGCTGGAAAAAGTTGGAAATTGGAATTTTGATATCTTTCTATTTGATAGACTAACAAAT
GGAAATAGTCTAGTAAGCTTAACCTTTCATTTATTTAGTCTTCATGGATTAATTGAGTACTTCCATTTAG
ATATGATGAAACTTCGTAGATTTTTAGTTATGATTCAAGAAGATTACCACAGTCAAAATCCTTACCATAA
CGCAGTCCACGCTGCGGATGTTACTCAGGCCATGCACTGTTACTTAAAGGAACCTAAGCTTGCCAATTCT
GTAACTCCTTGGGATATCTTGCTGAGCTTAATTGCAGCTGCCACTCATGATCTGGATCATCCAGGTGTTA
ATCAACCTTTCCTTATTAAAACTAACCATTACTTGGCAACTTTATACAAGAATACCTCAGTACTGGAAAA
TCACCACTGGAGATCTGCAGTGGGCTTATTGAGAGAATCAGGCTTATTCTCACATCTGCCATTAGAAAGC
AGGCAACAAATGGAGACACAGATAGGTGCTCTGATACTAGCCACAGACATCAGTCGCCAGAATGAGTATC
TGTCTTTGTTTAGGTCCCATTTGGATAGAGGTGATTTATGCCTAGAAGACACCAGACACAGACATTTGGT
TTTACAGATGGCTTTGAAATGTGCTGATATTTGTAACCCATGTCGGACGTGGGAATTAAGCAAGCAGTGG
AGTGAAAAAGTAACGGAGGAATTCTTCCATCAAGGAGATATAGAAAAAAAATATCATTTGGGTGTGAGTC
CACTTTGCGATCGTCACACTGAATCTATTGCCAACATCCAGATTGGTTTTATGACTTACCTAGTGGAGCC
TTTATTTACAGAATGGGCCAGGTTTTCCAATACAAGGCTATCCCAGACAATGCTTGGACACGTGGGGCTG
AATAAAGCCAGCTGGAAGGGACTGCAGAGAGAACAGTCGAGCAGTGAGGACACTGATGCTGCATTTGAGT
TGAACTCACAGTTATTACCTCAGGAAAATCGGTTATCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214749 representing NM_002603
Red=Cloning site Green=Tags(s)

MGITLIWCLALVLIKWITSKRRGAISYDSSDQTALYIRMLGDVRVRSRAGFESERRGSHPYIDFRIFHSQ
SEIEVSVSARNIRRLLSFQRYLRSSRFFRGTAVSNSLNILDDDYNGQAKCMLEKVGNWNFDIFLFDRLTN
GNSLVSLTFHLFSLHGLIEYFHLDMMKLRRFLVMIQEDYHSQNPYHNAVHAADVTQAMHCYLKEPKLANS
VTPWDILLSLIAAATHDLDHPGVNQPFLIKTNHYLATLYKNTSVLENHHWRSAVGLLRESGLFSHLPLES
RQQMETQIGALILATDISRQNEYLSLFRSHLDRGDLCLEDTRHRHLVLQMALKCADICNPCRTWELSKQW
SEKVTEEFFHQGDIEKKYHLGVSPLCDRHTESIANIQIGFMTYLVEPLFTEWARFSNTRLSQTMLGHVGL
NKASWKGLQREQSSSEDTDAAFELNSQLLPQENRLS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002603
ORF Size 1368 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002603.4
RefSeq Size 1739 bp
RefSeq ORF 1371 bp
Locus ID 5150
UniProt ID Q13946
Cytogenetics 8q13.1
Protein Families Druggable Genome
Protein Pathways Progesterone-mediated oocyte maturation, Purine metabolism
MW 52.5 kDa
Summary The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE7 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. provided by RefSeq, Jul 2011
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_002603" in other vectors (6)
SKU Description Size Price
RC214749L1 Lenti ORF clone of Human phosphodiesterase 7A (PDE7A), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC214749L2 Lenti ORF clone of Human phosphodiesterase 7A (PDE7A), transcript variant 1, mGFP tagged 10 ug
$757.00
RC214749L3 Lenti ORF clone of Human phosphodiesterase 7A (PDE7A), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC214749L4 Lenti ORF clone of Human phosphodiesterase 7A (PDE7A), transcript variant 1, mGFP tagged 10 ug
$757.00
RG214749 PDE7A (tGFP-tagged) - Human phosphodiesterase 7A (PDE7A), transcript variant 1 10 ug
$489.00 $657.00
SC303225 PDE7A (untagged)-Human phosphodiesterase 7A (PDE7A), transcript variant 1 10 ug
$457.00

Plasmid Map for RC214749

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214749
Circular map for RC214749

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products