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SRP72 (NM_006947) Human Tagged ORF Clone

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SKU
RC214727
SRP72 (Myc-DDK-tagged)-Human signal recognition particle 72kDa (SRP72)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $614.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SRP72
Synonyms BMFF; BMFS1; HEL103
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214727 representing NM_006947
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAGCGGCGGCAGCGGGGGGGTGTCAGTACCTGCGCTGTGGAGTGAAGTGAACCGGTATGGCCAGA
ACGGCGACTTCACGCGCGCTCTCAAGACCGTCAATAAGATACTACAGATCAACAAAGATGACGTAACTGC
CCTGCATTGTAAAGTGGTATGCCTTATCCAGAATGGAAGTTTCAAGGAAGCTTTGAATGTCATCAATACT
CACACCAAAGTGTTAGCCAATAACTCTCTCTCCTTTGAAAAGGCATATTGCGAGTACAGGCTGAACAGAA
TTGAGAATGCCTTGAAGACAATAGAAAGTGCCAACCAGCAGACAGACAAACTGAAGGAGCTTTATGGACA
AGTGTTATATCGTTTGGAACGCTATGATGAATGCTTAGCAGTGTATAGAGATCTCGTCCGAAACTCCCAA
GATGATTATGATGAGGAGAGGAAAACAAACCTTTCAGCAGTTGTTGCAGCTCAAAGCAATTGGGAAAAAG
TGGTTCCAGAGAACCTGGGCCTCCAAGAAGGCACACATGAGCTGTGCTACAACACTGCATGTGCACTGAT
AGGCCAAGGCCAGCTGAACCAGGCCATGAAAATCCTACAAAAAGCTGAAGATCTTTGCCGCCGTTCATTA
TCAGAAGACACTGATGGGACTGAGGAAGACCCACAGGCAGAACTGGCCATCATTCATGGTCAGATGGCTT
ATATTCTGCAGCTTCAGGGTCGAACAGAGGAGGCTTTGCAACTTTACAATCAAATAATAAAACTAAAACC
AACAGATGTGGGATTACTAGCTGTAATTGCAAATAACATCATTACCATTAACAAGGACCAAAATGTCTTT
GACTCCAAGAAGAAAGTGAAATTAACCAATGCGGAAGGAGTAGAGTTTAAGCTTTCCAAGAAACAACTAC
AAGCTATAGAATTTAACAAAGCTTTACTTGCTATGTACACAAACCAGGCTGAACAATGCCGCAAAATATC
TGCCAGTTTACAGTCCCAAAGTCCCGAGCATCTCTTACCTGTGTTAATCCAAGCTGCCCAGCTCTGCCGT
GAAAAGCAGCACACAAAAGCAATAGAGCTGCTTCAGGAATTTTCAGATCAGCATCCAGAAAATGCAGCTG
AAATTAAGCTGACCATGGCACAGTTGAAAATTTCTCAAGGTAATATTTCTAAAGCATGTCTAATATTGAG
AAGCATAGAGGAGTTAAAGCATAAACCAGGCATGGTATCTGCATTAGTTACCATGTATAGCCATGAAGAA
GATATTGATAGTGCCATTGAGGTCTTCACACAAGCTATCCAGTGGTATCAAAACCATCAGCCAAAATCTC
CTGCTCATTTGTCCTTGATAAGAGAAGCTGCAAACTTCAAACTCAAATATGGGCGGAAGAAGGAGGCAAT
TAGTGACCTACAACAGCTGTGGAAACAAAATCCAAAAGATATTCACACCCTGGCACAGCTTATTTCTGCT
TACTCACTTGTAGATCCAGAGAAAGCCAAAGCTCTTAGTAAACACTTGCCATCGTCAGATAGTATGTCTC
TAAAAGTAGATGTTGAGGCTCTTGAAAATTCTGCTGGTGCTACATACATTCGGAAGAAGGGTGGAAAAGT
TACTGGAGATAGTCAACCAAAGGAACAAGGACAGGGAGATTTGAAAAAGAAGAAAAAGAAAAAGAAGGGA
AAATTGCCTAAGAATTATGACCCAAAAGTTACCCCAGATCCAGAAAGATGGCTGCCAATGCGAGAACGTT
CTTACTACCGGGGAAGAAAGAAGGGTAAAAAGAAGGATCAGATTGGAAAAGGGACCCAGGGAGCAACTGC
AGGAGCTTCATCTGAACTGGATGCCAGTAAAACTGTGAGCAGCCCACCCACCTCCCCAAGACCTGGCAGT
GCTGCAACAGTATCTGCCTCTACAAGTAACATCATACCCCCAAGACACCAGAAACCTGCAGGGGCTCCAG
CAACAAAAAAGAAACAGCAACAGAAAAAGAAGAAAGGTGGAAAAGGTGGCTGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214727 representing NM_006947
Red=Cloning site Green=Tags(s)

MASGGSGGVSVPALWSEVNRYGQNGDFTRALKTVNKILQINKDDVTALHCKVVCLIQNGSFKEALNVINT
HTKVLANNSLSFEKAYCEYRLNRIENALKTIESANQQTDKLKELYGQVLYRLERYDECLAVYRDLVRNSQ
DDYDEERKTNLSAVVAAQSNWEKVVPENLGLQEGTHELCYNTACALIGQGQLNQAMKILQKAEDLCRRSL
SEDTDGTEEDPQAELAIIHGQMAYILQLQGRTEEALQLYNQIIKLKPTDVGLLAVIANNIITINKDQNVF
DSKKKVKLTNAEGVEFKLSKKQLQAIEFNKALLAMYTNQAEQCRKISASLQSQSPEHLLPVLIQAAQLCR
EKQHTKAIELLQEFSDQHPENAAEIKLTMAQLKISQGNISKACLILRSIEELKHKPGMVSALVTMYSHEE
DIDSAIEVFTQAIQWYQNHQPKSPAHLSLIREAANFKLKYGRKKEAISDLQQLWKQNPKDIHTLAQLISA
YSLVDPEKAKALSKHLPSSDSMSLKVDVEALENSAGATYIRKKGGKVTGDSQPKEQGQGDLKKKKKKKKG
KLPKNYDPKVTPDPERWLPMRERSYYRGRKKGKKKDQIGKGTQGATAGASSELDASKTVSSPPTSPRPGS
AATVSASTSNIIPPRHQKPAGAPATKKKQQQKKKKGGKGGW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006947
ORF Size 2013 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006947.4
RefSeq Size 3852 bp
RefSeq ORF 2016 bp
Locus ID 6731
UniProt ID O76094
Cytogenetics 4q12
Domains TPR
Protein Pathways Protein export
MW 74.4 kDa
Summary This gene encodes the 72 kDa subunit of the signal recognition particle (SRP), a ribonucleoprotein complex that mediates the targeting of secretory proteins to the endoplasmic reticulum (ER). The SRP complex consists of a 7S RNA and 6 protein subunits: SRP9, SRP14, SRP19, SRP54, SRP68, and SRP72, that are bound to the 7S RNA as monomers or heterodimers. SRP has at least 3 distinct functions that can be associated with the protein subunits: signal recognition, translational arrest, and ER membrane targeting by interaction with the docking protein. Mutations in this gene are associated with familial bone marrow failure. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jun 2012
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"NM_006947" in other vectors (6)
SKU Description Size Price
RC214727L1 Lenti ORF clone of Human signal recognition particle 72kDa (SRP72), Myc-DDK-tagged 10 ug
$914.00
RC214727L2 Lenti ORF clone of Human signal recognition particle 72kDa (SRP72), mGFP tagged 10 ug
$914.00
RC214727L3 Lenti ORF clone of Human signal recognition particle 72kDa (SRP72), Myc-DDK-tagged 10 ug
$914.00
RC214727L4 Lenti ORF clone of Human signal recognition particle 72kDa (SRP72), mGFP tagged 10 ug
$914.00
RG214727 SRP72 (tGFP-tagged) - Human signal recognition particle 72kDa (SRP72) 10 ug
$789.00 $814.00
SC115795 SRP72 (untagged)-Human signal recognition particle 72kDa (SRP72) 10 ug
$615.00

Plasmid Map for RC214727

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214727
Circular map for RC214727

file-alt Citations

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