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SRP72 (NM_006947) Human Untagged Clone

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SKU
SC115795
SRP72 (untagged)-Human signal recognition particle 72kDa (SRP72)
  $615.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SRP72
Synonyms BMFF; BMFS1; HEL103
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_006947 edited
GCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGT
AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTCTCCTCCAAGATG
GCGAGCGGCGGCAGCGGGGGGGTGTCAGTACCTGCGCTGTGGAGTGAAGTGAACCGGTAT
GGCCAGAACGGCGACTTCACGCGCGCTCTCAAGACCGTCAATAAGATACTACAGATCAAC
AAAGATGACGTAACTGCCCTGCATTGTAAAGTGGTATGCCTTATCCAGAATGGAAGTTTC
AAGGAAGCTTTGAATGTCATCAATACTCACACCAAAGTGTTAGCCAATAACTCTCTCTCC
TTTGAAAAGGCATATTGCGAGTACAGGCTGAACAGAATTGAGAATGCCTTGAAGACAATA
GAAAGTGCCAACCAGCAGACAGACAAACTGAAGGAGCTTTATGGACAAGTGTTATATCGT
TTGGAACGCTATGATGAATGCTTAGCAGTGTATAGAGATCTCGTCCGAAACTCCCAAGAT
GATTATGATGAGGAGAGGAAAACAAACCTTTCAGCAGTTGTTGCAGCTCAAAGCAATTGG
GAAAAAGTGGTTCCAGAGAACCTGGGCCTCCAAGAAGGCACACATGAGCTGTGCTACAAC
ACTGCATGTGCACTGATAGGCCAAGGCCAGCTGAACCAGGCCATGAAAATCCTACAAAAA
GCTGAAGATCTTTGCCGCCGTTCATTATCAGAAGACACTGATGGGACTGAGGAAGACCCA
CAGGCAGAACTGGCCATCATTCATGGTCAGATGGCTTATATTCTGCAGCTTCAGGGTCGA
ACAGAGGAGGCTTTGCAACTTTACAATCAAATAATAAAACTAAAACCAACAGATGTGGGA
TTACTAGCTGTAATTGCAAATAACATCATTACCATTAACAAGGACCAAAATGTCTTTGAC
TCCAAGAAGAAAGTGAAATTAACCAATGCGGAAGGAGTAGAGTTTAAGCTTTCCAAGAAA
CAACTACAAGCTATAGAATTTAACAAAGCTTTACTTGCTATGTACACAAACCAGGCTGAA
CAATGCCGCAAAATATCTGCCAGTTTACAGTCCCAAAGTCCCGAGCATCTCTTACCTGTG
TTAATCCAAGCTGCCCAGCTCTGCCGTGAAAAGCAGCACACAAAAGCAATAGAGCTGCTT
CAGGAATTTTCAGATCAGCATCCAGAAAATGCAGCTGAAATTAAGCTGACCATGGCACAG
TTGAAAATTTCTCAAGGTAATATTTCTAAAGCATGTCTAATATTGAGAAGCATAGAGGAG
TTAAAGCATAAACCAGGCATGGTATCTGCATTAGTTACCATGTATAGCCATGAAGAAGAT
ATTGATAGTGCCATTGAGGTCTTCACACAAGCTATCCAGTGGTATCAAAACCATCAGCCA
AAATCTCCTGCTCATTTGTCCTTGATAAGAGAAGCTGCAAACTTCAAACTCAAATATGGG
CGGAAGAAGGAGGCAATTAGTGACCTACAACAGCTGTGGAAACAAAATCCAAAAGATATT
CACACCCTGGCACAGCTTATTTCTGCTTACTCACTTGTAGATCCAGAGAAAGCCAAAGCT
CTTAGTAAACACTTGCCATCGTCAGATAGTATGTCTCTAAAAGTAGATGTTGAGGCTCTT
GAAAATTCTGCTGGTGCTACATACATTCGGAAGAAGGGTGGAAAAGTTACTGGAGATAGT
CAACCAAAGGAACAAGGACAGGGAGATTTGAAAAAGAAGAAAAAGAAAAAGAAGGGAAAA
TTGCCTAAGAATTATGACCCAAAAGTTACCCCAGATCCAGAAAGATGGCTGCCAATGCGA
GAACGTTCTTACTACCGGGGAAGAAAGAAGGGTAAAAAGAAGGATCAGATTGGAAAAGGG
ACCCAGGGAGCAACTGCAGGAGCTTCATCTGAACTGGATGCCAGTAAAACTGTGAGCAGC
CCACCCACCTCCCCAAGACCTGGCAGTGCTGCAACAGTATCTGCCTCTACAAGTAACATC
ATACCCCCAAGACACCAGAAACCTGCAGGGGCTCCAGCAACAAAAAAGAAACAGCAACAG
AAAAAGAAGAAAGGTGGAAAAGGTGGCTGGTGATGAGAATATTCTTGTTGCAGGCTGTTT
TTAAACTAGTGTCAGTGACACTAGGAATATAATAAAGGTAACACAGCAAGAAGCACAGAA
CTACTCCCTCTTCATCTCCATATTTTCATAATTTCTTGTGTTTCAAATAGGGAAACATCT
TCCTCAAAGTCTGCCTAGTGAGATATGGCCTACTGGTTGCCTCATAGCTTTGTACAGATT
ATGAGGACTGAAAATAATTGGGCATTTACCCATCTTGGTATCTGTTGTATCCTTTATCTG
TGTGTGCTGATTTGATCTTTTTTCAGTTTCACATACCTTATCTAAGGTTTCCCAGGATTT
AAACAGAAACTACTT
5' Read Nucleotide Sequence
>OriGene 5' read for NM_006947 unedited
TCACCAATTTGGTATACGACTCACTATAGNNGGCGGCCGCGNAATTCGGCACGAGGCCTC
GTCTCCTCCAAATGGCGAGCGGCGGCAGCGGGGGGGTGTCAGTACCTGCGCTGTGGAGTG
AAGTGAACCGGTATGGCCAGAACGGCGACTTCACGCGCGCTCTCAAGACCGTCAATAAGA
TACTACAGATCAACAAAGATGACGTAACTGCCCTGCATTGTAAAGTGGTATGCCTTATCC
AGAATGGAAGTTTCAAGGAAGCTTTGAATGTCATCAATACTCACACCAAAGTGTTAGCCA
ATAACTCTCTCTCCTTTGAAAAGGCATATTGCGAGTACAGGCTGAACAGAATTGAGAATG
CCTTGAAGACAATAGAAAGTGCCAACCAGCAGACAGACAAACTGAAGGAGCTTTATGGAC
AAGTGTTATATCGTTTGGAACGCTATGATGAATGCTTAGCAGTGTATAGAGATCTCGTCC
GAAACTCCCAAGATGATTATGATGAGGAGAGGAAAACAAACCTTTCAGCAGTTGTTGCAG
CTCAAAGCAATTGGGAAAAAGTGGTTCCAGAGAACCTGNGCCTCCAAGAAGGCACACATG
AGCTGTGCTACAACACTGCATGTGCACTGATAGGCCAAGGCCAGCTGAACCAGGCCATGA
AAATCCTACATAAAGCTGAAGATCCTTTGCCGCCGTTCATTATCAGAAGACACTGATGGG
ACTGAAGAAGACCCACAGGCAGAACTGGCCATCATTCATGGGCAGAAGGCTTATATTCTG
CAGCTTCAGGGTCGAACAGAGGGAGCTTTGCAACTTTACATTAAATAATAAAACTAAACC
CACCGATGTGGGATTACTAGCTGTAATTGCAATAACATTCTTCCCTTAACACGAACCAAA
TGTCTTTTACTCCCAGAACAAGTGAAATTACCAT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_006947 unedited
CCCCCCCGGGCCCCCCCCCNCNTTTTACCCCCNNNTTTTTGGGCANNCCTTTCTTTTTTT
TGTGGGCCTNCCTTTNATGNAANACATNCATGTATTAAAAAGTCCCTCTTTATCACATGA
TATTTCTAAGCAGNATATTTAGAATGCTCAGAAGATTCCACTTTCAGTCTCCTAAGAACA
TAATTTAAAGTGGGAACTTGAACAGNAAACAAGTATCTTCAGACTCCAGCTGAAATCATA
GAAGTAGTTTCTGTTTAAATCCTGGGAAACCTTAGATAAGGTATGTGAAACTGAAAAAAG
ATCAAATCAGCACACACAGATAAAGGATACAACAGATACCAAGATGGGTAAATGCCCAAT
TATTTTCAGTCCTCATAATCTGTACAAAGCTATGAGGCAACCAGTAGGCCATATCTCACT
AGGCAGACTTTGACGAAGATGTTTCCCTATTTGAAACACAAGAAATTATGAAAATATGGA
GATGAAGAGGGAGTAGTTCTGTGCTTCTTGCTGTGTTACCTTTATTATATTCCTAGTGTC
ACTGACACTAGTTTAAAAACAGCCTGCAACAAGAATATTCTCATCACCAGCCACCTTTTC
CACCTTTCTTCTTTTTCTGTTGCTGTTTCTTTTTTGTTGCTGGAGCCCCTGCAGGTTTCT
GGTGTCTTGGGGGTATGATGTTACTTGTAGAGGCAGATACTGTTGCAGCACTGCCAGGTC
TTGGGGAGGTGGGTGGGCTGCTCACAGTTTTACTGGCATCCAGTTCAGATGAAGCTCCTG
CAGTTGCTCCCTGGGTCCCTTTTTCCATCTGATCCTTCTTTTTACCCCTCTTTCTTCCCC
GGTAGTAAAACGTTCTCGCATTGGCAGCCATCTTTCTGGGTCTGGGGGTAACTTTGGGTC
ATAATTCTTAGGCAATTTCCCTTCTTTTCTTTTTCCTTCTTTTCAAACTCCCTGTCCTTG
GTC
Restriction Sites NotI-NotI |
ACCN NM_006947
Insert Size 2690 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006947.2, NP_008878.2
RefSeq Size 3852 bp
RefSeq ORF 2016 bp
Locus ID 6731
UniProt ID O76094
Cytogenetics 4q12
Domains TPR
Protein Pathways Protein export
Summary This gene encodes the 72 kDa subunit of the signal recognition particle (SRP), a ribonucleoprotein complex that mediates the targeting of secretory proteins to the endoplasmic reticulum (ER). The SRP complex consists of a 7S RNA and 6 protein subunits: SRP9, SRP14, SRP19, SRP54, SRP68, and SRP72, that are bound to the 7S RNA as monomers or heterodimers. SRP has at least 3 distinct functions that can be associated with the protein subunits: signal recognition, translational arrest, and ER membrane targeting by interaction with the docking protein. Mutations in this gene are associated with familial bone marrow failure. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jun 2012
Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1).
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Documents
Resources
"NM_006947" in other vectors (6)
SKU Description Size Price
RC214727 SRP72 (Myc-DDK-tagged)-Human signal recognition particle 72kDa (SRP72) 10 ug
$614.00
RC214727L1 Lenti ORF clone of Human signal recognition particle 72kDa (SRP72), Myc-DDK-tagged 10 ug
$914.00
RC214727L2 Lenti ORF clone of Human signal recognition particle 72kDa (SRP72), mGFP tagged 10 ug
$914.00
RC214727L3 Lenti ORF clone of Human signal recognition particle 72kDa (SRP72), Myc-DDK-tagged 10 ug
$914.00
RC214727L4 Lenti ORF clone of Human signal recognition particle 72kDa (SRP72), mGFP tagged 10 ug
$914.00
RG214727 SRP72 (tGFP-tagged) - Human signal recognition particle 72kDa (SRP72) 10 ug
$789.00 $814.00

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