CDC40 (NM_015891) Human Tagged ORF Clone

SKU RC214712

CDC40 (Myc-DDK-tagged)-Human cell division cycle 40 homolog (S. cerevisiae) (CDC40)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: CDC40
• Synonyms: EHB3; PCH15; PRP17; PRPF17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214712 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCGGCTGCGATTGCAGCTCTGGCCGCTTCCTATGGTTCGGGTTCAGGGTCCGAATCGGACTCGGACA; GTGAGAGCAGTCGGTGTCCGCTGCCAGCCGCCGACTCCCTCATGCACTTGACTAAATCGCCTTCATCAAA; GCCGTCTCTAGCAGTGGCAGTGGACTCGGCTCCGGAGGTGGCAGTTAAGGAAGATTTGGAGACTGGAGTT; CACCTTGACCCTGCCGTCAAAGAAGTTCAGTATAATCCTACCTATGAGACCATGTTTGCTCCTGAGTTTG; GACCAGAAAATCCCTTTAGGACACAGCAAATGGCTGCCCCTAGAAATATGCTTTCTGGATATGCCGAACC; AGCTCATATCAATGATTTCATGTTTGAGCAGCAAAGGAGAACTTTTGCAACATATGGTTATGCATTAGAC; CCTTCATTAGATAATCATCAAGTGTCTGCTAAATATATTGGTTCTGTAGAAGAAGCTGAAAAAAATCAAG; GTTTAACTGTATTTGAAACTGGTCAGAAGAAAACAGAAAAGAGGAAAAAGTTTAAAGAAAATGATGCATC; CAATATTGATGGTTTTTTGGGACCATGGGCAAAATATGTGGATGAAAAAGATGTAGCCAAACCTTCAGAA; GAAGAGCAAAAAGAATTGGATGAAATCACAGCAAAGAGGCAGAAAAAAGGAAAACAGGAAGAAGAGAAAC; CTGGGGAGGAGAAGACAATCTTACATGTTAAAGAAATGTATGACTATCAAGGCAGGTCCTATCTTCACAT; ACCTCAGGATGTTGGTGTTAATCTACGGTCAACTATGCCACCTGAGAAGTGTTATCTTCCCAAAAAACAA; ATTCATGTGTGGTCTGGACACACAAAGGGCGTCAGTGCAGTCAGATTGTTTCCTCTCTCTGGCCATTTAT; TGCTGTCTTGTTCCATGGACTGTAAAATTAAGCTATGGGAGGTTTATGGAGAACGGCGCTGTCTGAGAAC; ATTTATTGGTCACAGTAAGGCTGTTAGGGATATCTGCTTCAATACTGCAGGAACACAGTTCCTCAGTGCA; GCCTATGACAGGTATCTTAAGCTCTGGGACACTGAGACAGGACAGTGTATATCAAGATTTACAAACCGAA; AAGTACCTTATTGTGTCAAATTCAATCCTGATGAAGATAAGCAAAATCTCTTTGTGGCTGGGATGTCTGA; TAAGAAGATTGTGCAATGGGACATTCGAAGTGGAGAAATTGTGCAGGAATATGATCGGCATTTGGGAGCT; GTCAACACCATTGTTTTTGTGGATGAGAATAGGAGATTTGTGAGCACATCTGATGATAAAAGCCTAAGAG; TTTGGGAATGGGATATCCCTGTGGATTTCAAGTACATAGCAGAACCCAGTATGCACTCAATGCCTGCAGT; GACTTTGTCTCCAAATGGAAAATGGCTAGCATGCCAATCAATGGACAACCAAATCTTAATTTTTGGAGCA; CAGAACAGATTTAGATTAAATAAGAAAAAAATTTTTAAGGGCCATATGGTAGCAGGCTATGCTTGTCAGG; TGGACTTTTCACCAGACATGAGTTATGTGATTTCAGGAGATGGAAATGGAAAATTAAACATTTGGGACTG; GAAGACCACAAAACTCTACAGTCGATTTAAAGCTCATGATAAAGTGTGTATAGGTGCAGTGTGGCATCCT; CATGAAACTTCTAAGGTCATAACATGTGGTTGGGATGGTCTCATTAAATTGTGGGAT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214712 protein sequence; Red=Cloning site Green=Tags(s); ; MSAAIAALAASYGSGSGSESDSDSESSRCPLPAADSLMHLTKSPSSKPSLAVAVDSAPEVAVKEDLETGV; HLDPAVKEVQYNPTYETMFAPEFGPENPFRTQQMAAPRNMLSGYAEPAHINDFMFEQQRRTFATYGYALD; PSLDNHQVSAKYIGSVEEAEKNQGLTVFETGQKKTEKRKKFKENDASNIDGFLGPWAKYVDEKDVAKPSE; EEQKELDEITAKRQKKGKQEEEKPGEEKTILHVKEMYDYQGRSYLHIPQDVGVNLRSTMPPEKCYLPKKQ; IHVWSGHTKGVSAVRLFPLSGHLLLSCSMDCKIKLWEVYGERRCLRTFIGHSKAVRDICFNTAGTQFLSA; AYDRYLKLWDTETGQCISRFTNRKVPYCVKFNPDEDKQNLFVAGMSDKKIVQWDIRSGEIVQEYDRHLGA; VNTIVFVDENRRFVSTSDDKSLRVWEWDIPVDFKYIAEPSMHSMPAVTLSPNGKWLACQSMDNQILIFGA; QNRFRLNKKKIFKGHMVAGYACQVDFSPDMSYVISGDGNGKLNIWDWKTTKLYSRFKAHDKVCIGAVWHP; HETSKVITCGWDGLIKLWD; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015891
• ORF Size: 1737 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015891.3
• RefSeq Size: 3866 bp
• RefSeq ORF: 1740 bp
• Locus ID: 51362
• UniProt ID: O60508
• Cytogenetics: 6q21
• Domains: WD40
• Protein Pathways: Spliceosome
• MW: 65.5 kDa
• Summary: Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp17 protein, which functions in two different cellular processes: pre-mRNA splicing and cell cycle progression. It suggests that this protein may play a role in cell cycle progression. [provided by RefSeq, Jul 2008]