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UBA6 (NM_018227) Human Tagged ORF Clone

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SKU
RC214483
UBA6 (Myc-DDK-tagged)-Human ubiquitin-like modifier activating enzyme 6 (UBA6)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $916.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol UBA6
Synonyms E1-L2; MOP-4; UBE1L2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214483 representing NM_018227
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGGATCCGAGCCTGTGGCCGCCCATCAGGGGGAAGAGGCGTCCTGTTCTTCCTGGGGGACTGGCA
GCACAAATAAAAATTTGCCCATTATGTCAACAGCATCTGTGGAAATCGATGATGCATTGTATAGTCGACA
GAGGTACGTTCTTGGAGACACAGCAATGCAGAAGATGGCCAAGTCCCATGTTTTCTTAAGTGGGATGGGT
GGTCTTGGTTTGGAAATTGCAAAGAATCTTGTTCTTGCAGGGATTAAGGCAGTTACAATTCATGATACAG
AAAAATGCCAAGCATGGGATCTAGGAACCAACTTCTTTCTCAGTGAAGATGATGTTGTTAATAAGAGAAA
CAGGGCTGAAGCTGTACTTAAACATATTGCAGAACTAAATCCATACGTTCATGTCACATCATCTTCTGTT
CCTTTCAATGAGACCACAGATCTCTCCTTTTTAGATAAATACCAGTGTGTAGTATTGACTGAGATGAAAC
TTCCATTGCAGAAGAAGATCAATGACTTTTGCCGTTCTCAGTGCCCTCCAATTAAGTTTATCAGTGCAGA
TGTACATGGAATTTGGTCAAGGTTATTTTGTGATTTCGGTGATGAATTTGAAGTTTTAGATACAACAGGA
GAAGAACCAAAAGAAATTTTCATTTCAAACATAACGCAAGCAAATCCTGGCATTGTTACTTGCCTTGAAA
ATCATCCTCACAAACTGGAGACAGGACAATTCCTAACATTTCGAGAAATTAATGGAATGACAGGTTTAAA
TGGATCTATACAACAAATAACGGTGATATCGCCATTTTCTTTTAGTATTGGTGACACCACAGAACTGGAA
CCATATTTACATGGAGGCATAGCTGTCCAAGTTAAGACTCCTAAAACAGTTTTTTTTGAATCACTGGAGA
GGCAGTTAAAACATCCAAAGTGCCTTATTGTGGATTTTAGCAACCCTGAGGCACCTTTAGAGATTCACAC
AGCTATGCTTGCCTTGGACCAGTTTCAGGAGAAATACAGTCGCAAGCCAAATGTTGGATGCCAACAAGAT
TCAGAAGAACTGTTGAAACTAGCAACATCTATAAGTGAAACCTTGGAAGAGAAGCCTGATGTAAATGCTG
ACATTGTGCATTGGCTCTCTTGGACTGCCCAAGGCTTTTTATCTCCACTTGCTGCAGCAGTAGGAGGTGT
TGCCAGCCAAGAAGTATTGAAAGCTGTAACAGGAAAATTTTCTCCTTTGTGCCAGTGGTTATATCTTGAA
GCAGCAGATATTGTTGAATCACTAGGCAAACCTGAATGTGAAGAATTTCTCCCACGAGGAGATAGATATG
ATGCCTTAAGAGCTTGCATTGGAGACACTTTGTGTCAGAAACTGCAAAATTTAAACATCTTCTTAGTAGG
GTGTGGAGCCATAGGCTGTGAAATGTTGAAAAATTTTGCTTTACTTGGTGTTGGCACAAGCAAAGAGAAA
GGAATGATTACAGTTACAGATCCTGACTTGATAGAGAAATCCAACTTAAATAGACAGTTCCTATTTCGTC
CTCATCACATACAGAAACCTAAAAGCTACACTGCTGCTGATGCTACTCTGAAAATAAATTCTCAAATAAA
GATAGATGCACACCTGAACAAAGTATGTCCAACCACTGAGACCATTTACAATGATGAGTTCTATACTAAA
CAAGATGTAATTATTACAGCATTAGATAATGTGGAAGCCAGGAGATACGTAGACAGTCGTTGCTTAGCAA
ATCTAAGGCCTCTTTTAGATTCTGGAACAATGGGCACTAAGGGACACACTGAAGTTATTGTACCGCATTT
GACTGAGTCTTACAATAGTCATCGGGATCCCCCAGAAGAGGAAATACCATTTTGTACTCTAAAATCCTTT
CCAGCTGCTATTGAACATACCATACAGTGGGCAAGAGATAAGTTTGAAAGTTCCTTTTCCCACAAACCTT
CATTGTTTAACAAATTTTGGCAAACCTATTCATCTGCAGAAGAAGTCTTACAGAAGATACAGAGTGGACA
CAGTTTAGAAGGCTGTTTTCAAGTTATAAAGTTACTTAGCAGAAGACCTAGAAATTGGTCCCAGTGTGTA
GAATTAGCAAGATTAAAGTTTGAAAAATATTTTAACCATAAGGCTCTTCAGCTTCTTCACTGTTTCCCTC
TGGACATACGATTAAAAGATGGCAGTTTATTTTGGCAGTCACCAAAGAGGCCACCCTCTCCAATAAAATT
TGATTTAAATGAGCCTTTGCACCTCAGTTTCCTTCAGAATGCTGCAAAACTATATGCTACAGTATATTGT
ATTCCATTTGCAGAAGAGGACTTATCAGCAGATGCCCTCTTGAATATTCTTTCAGAAGTAAAGATTCAGG
AATTCAAGCCTTCCAATAAGGTTGTTCAAACAGATGAAACTGCAAGGAAACCAGACCATGTTCCTATTAG
CAGTGAAGATGAGAGGAATGCAATTTTCCAACTAGAAAAGGCTATTTTATCTAATGAAGCCACCAAAAGT
GACCTTCAGATGGCAGTGCTTTCATTTGAAAAAGATGATGATCATAATGGACACATAGATTTCATCACAG
CTGCATCAAATCTTCGTGCCAAAATGTACAGCATTGAACCAGCTGACCGTTTCAAAACAAAGCGCATAGC
TGGTAAAATTATACCTGCTATAGCAACAACCACTGCTACAGTTTCTGGCTTGGTTGCCTTGGAGATGATC
AAAGTAACTGGTGGCTATCCATTTGAAGCTTACAAAAATTGTTTTCTTAACTTAGCCATTCCAATTGTAG
TATTTACAGAGACAACTGAAGTAAGGAAAACTAAAATCAGAAATGGAATATCATTTACAATTTGGGATCG
ATGGACCGTACATGGAAAAGAAGATTTCACCCTCTTGGATTTCATAAATGCAGTCAAAGAGAAGTATGGA
ATTGAGCCAACAATGGTGGTACAGGGAGTCAAAATGCTTTATGTTCCTGTAATGCCTGGTCATGCAAAAA
GATTGAAGTTAACAATGCATAAACTTGTAAAACCTACTACTGAAAAGAAATATGTGGATCTTACTGTGTC
ATTTGCTCCAGACATTGATGGAGATGAAGATTTGCCGGGACCTCCAGTAAGATACTACTTCAGTCATGAC
ACTGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214483 representing NM_018227
Red=Cloning site Green=Tags(s)

MEGSEPVAAHQGEEASCSSWGTGSTNKNLPIMSTASVEIDDALYSRQRYVLGDTAMQKMAKSHVFLSGMG
GLGLEIAKNLVLAGIKAVTIHDTEKCQAWDLGTNFFLSEDDVVNKRNRAEAVLKHIAELNPYVHVTSSSV
PFNETTDLSFLDKYQCVVLTEMKLPLQKKINDFCRSQCPPIKFISADVHGIWSRLFCDFGDEFEVLDTTG
EEPKEIFISNITQANPGIVTCLENHPHKLETGQFLTFREINGMTGLNGSIQQITVISPFSFSIGDTTELE
PYLHGGIAVQVKTPKTVFFESLERQLKHPKCLIVDFSNPEAPLEIHTAMLALDQFQEKYSRKPNVGCQQD
SEELLKLATSISETLEEKPDVNADIVHWLSWTAQGFLSPLAAAVGGVASQEVLKAVTGKFSPLCQWLYLE
AADIVESLGKPECEEFLPRGDRYDALRACIGDTLCQKLQNLNIFLVGCGAIGCEMLKNFALLGVGTSKEK
GMITVTDPDLIEKSNLNRQFLFRPHHIQKPKSYTAADATLKINSQIKIDAHLNKVCPTTETIYNDEFYTK
QDVIITALDNVEARRYVDSRCLANLRPLLDSGTMGTKGHTEVIVPHLTESYNSHRDPPEEEIPFCTLKSF
PAAIEHTIQWARDKFESSFSHKPSLFNKFWQTYSSAEEVLQKIQSGHSLEGCFQVIKLLSRRPRNWSQCV
ELARLKFEKYFNHKALQLLHCFPLDIRLKDGSLFWQSPKRPPSPIKFDLNEPLHLSFLQNAAKLYATVYC
IPFAEEDLSADALLNILSEVKIQEFKPSNKVVQTDETARKPDHVPISSEDERNAIFQLEKAILSNEATKS
DLQMAVLSFEKDDDHNGHIDFITAASNLRAKMYSIEPADRFKTKRIAGKIIPAIATTTATVSGLVALEMI
KVTGGYPFEAYKNCFLNLAIPIVVFTETTEVRKTKIRNGISFTIWDRWTVHGKEDFTLLDFINAVKEKYG
IEPTMVVQGVKMLYVPVMPGHAKRLKLTMHKLVKPTTEKKYVDLTVSFAPDIDGDEDLPGPPVRYYFSHD
TD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018227
ORF Size 3156 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018227.6
RefSeq Size 6462 bp
RefSeq ORF 3159 bp
Locus ID 55236
UniProt ID A0AVT1, A1LT96
Cytogenetics 4q13.2
Domains ThiF, UBACT
Protein Pathways Ubiquitin mediated proteolysis
MW 117.8 kDa
Summary Modification of proteins with ubiquitin (UBB; MIM 191339) or ubiquitin-like proteins controls many signaling networks and requires a ubiquitin-activating enzyme (E1), a ubiquitin conjugating enzyme (E2), and a ubiquitin protein ligase (E3). UBE1L2 is an E1 enzyme that initiates the activation and conjugation of ubiquitin-like proteins (Jin et al., 2007 PubMed 17597759).supplied by OMIM, Mar 2008
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Resources
"NM_018227" in other vectors (7)
SKU Description Size Price
RC214483L1 Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 6 (UBA6), Myc-DDK-tagged 10 ug
$1,216.00
RC214483L2 Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 6 (UBA6), mGFP tagged 10 ug
$1,216.00
RC214483L3 Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 6 (UBA6), Myc-DDK-tagged 10 ug
$1,216.00
RC214483L4 Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 6 (UBA6), mGFP tagged 10 ug
$1,216.00
RG214483 UBA6 (tGFP-tagged) - Human ubiquitin-like modifier activating enzyme 6 (UBA6) 10 ug
$789.00 $1,116.00
SC111323 UBA6 (untagged)-Human ubiquitin-like modifier activating enzyme 6 (UBA6) 10 ug
$1,778.00
SC318471 UBA6 (untagged)-Human ubiquitin-like modifier activating enzyme 6 (UBA6) 10 ug
$917.00

Plasmid Map for RC214483

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214483
Circular map for RC214483

file-alt Citations

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