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UBA6 (NM_018227) Human Untagged Clone

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SKU
SC318471
UBA6 (untagged)-Human ubiquitin-like modifier activating enzyme 6 (UBA6)
  $917.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol UBA6
Synonyms E1-L2; MOP-4; UBE1L2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC318471 representing NM_018227.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGAAGGATCCGAGCCTGTGGCCGCCCATCAGGGGGAAGAGGCGTCCTGTTCTTCCTGGGGGACTGGC
AGCACAAATAAAAATTTGCCCATTATGTCAACAGCATCTGTGGAAATCGATGATGCATTGTATAGTCGA
CAGAGGTACGTTCTTGGAGACACAGCAATGCAGAAGATGGCCAAGTCCCATGTTTTCTTAAGTGGGATG
GGTGGTCTTGGTTTGGAAATTGCAAAGAATCTTGTTCTTGCAGGGATTAAGGCAGTTACAATTCATGAT
ACAGAAAAATGCCAAGCATGGGATCTAGGAACCAACTTCTTTCTCAGTGAAGATGATGTTGTTAATAAG
AGAAACAGGGCTGAAGCTGTACTTAAACATATTGCAGAACTAAATCCATACGTTCATGTCACATCATCT
TCTGTTCCTTTCAATGAGACCACAGATCTCTCCTTTTTAGATAAATACCAGTGTGTAGTATTGACTGAG
ATGAAACTTCCATTGCAGAAGAAGATCAATGACTTTTGCCGTTCTCAGTGCCCTCCAATTAAGTTTATC
AGTGCAGATGTACATGGAATTTGGTCAAGGTTATTTTGTGATTTCGGTGATGAATTTGAAGTTTTAGAT
ACAACAGGAGAAGAACCAAAAGAAATTTTCATTTCAAACATAACGCAAGCAAATCCTGGCATTGTTACT
TGCCTTGAAAATCATCCTCACAAACTGGAGACAGGACAATTCCTAACATTTCGAGAAATTAATGGAATG
ACAGGTTTAAATGGATCTATACAACAAATAACGGTGATATCGCCATTTTCTTTTAGTATTGGTGACACC
ACAGAACTGGAACCATATTTACATGGAGGCATAGCTGTCCAAGTTAAGACTCCTAAAACAGTTTTTTTT
GAATCACTGGAGAGGCAGTTAAAACATCCAAAGTGCCTTATTGTGGATTTTAGCAACCCTGAGGCACCT
TTAGAGATTCACACAGCTATGCTTGCCTTGGACCAGTTTCAGGAGAAATACAGTCGCAAGCCAAATGTT
GGATGCCAACAAGATTCAGAAGAACTGTTGAAACTAGCAACATCTATAAGTGAAACCTTGGAAGAGAAG
CCTGATGTAAATGCTGACATTGTGCATTGGCTCTCTTGGACTGCCCAAGGCTTTTTATCTCCACTTGCT
GCAGCAGTAGGAGGTGTTGCCAGCCAAGAAGTATTGAAAGCTGTAACAGGAAAATTTTCTCCTTTGTGC
CAGTGGTTATATCTTGAAGCAGCAGATATTGTTGAATCACTAGGCAAACCTGAATGTGAAGAATTTCTC
CCACGAGGAGATAGATATGATGCCTTAAGAGCTTGCATTGGAGACACTTTGTGTCAGAAACTGCAAAAT
TTAAACATCTTCTTAGTAGGGTGTGGAGCCATAGGCTGTGAAATGTTGAAAAATTTTGCTTTACTTGGT
GTTGGCACAAGCAAAGAGAAAGGAATGATTACAGTTACAGATCCTGACTTGATAGAGAAATCCAACTTA
AATAGACAGTTCCTATTTCGTCCTCATCACATACAGAAACCTAAAAGCTACACTGCTGCTGATGCTACT
CTGAAAATAAATTCTCAAATAAAGATAGATGCACACCTGAACAAAGTATGTCCAACCACTGAGACCATT
TACAATGATGAGTTCTATACTAAACAAGATGTAATTATTACAGCATTAGATAATGTGGAAGCCAGGAGA
TACGTAGACAGTCGTTGCTTAGCAAATCTAAGGCCTCTTTTAGATTCTGGAACAATGGGCACTAAGGGA
CACACTGAAGTTATTGTACCGCATTTGACTGAGTCTTACAATAGTCATCGGGATCCCCCAGAAGAGGAA
ATACCATTTTGTACTCTAAAATCCTTTCCAGCTGCTATTGAACATACCATACAGTGGGCAAGAGATAAG
TTTGAAAGTTCCTTTTCCCACAAACCTTCATTGTTTAACAAATTTTGGCAAACCTATTCATCTGCAGAA
GAAGTCTTACAGAAGATACAGAGTGGACACAGTTTAGAAGGCTGTTTTCAAGTTATAAAGTTACTTAGC
AGAAGACCTAGAAATTGGTCCCAGTGTGTAGAATTAGCAAGATTAAAGTTTGAAAAATATTTTAACCAT
AAGGCTCTTCAGCTTCTTCACTGTTTCCCTCTGGACATACGATTAAAAGATGGCAGTTTATTTTGGCAG
TCACCAAAGAGGCCACCCTCTCCAATAAAATTTGATTTAAATGAGCCTTTGCACCTCAGTTTCCTTCAG
AATGCTGCAAAACTATATGCTACAGTATATTGTATTCCATTTGCAGAAGAGGACTTATCAGCAGATGCC
CTCTTGAATATTCTTTCAGAAGTAAAGATTCAGGAATTCAAGCCTTCCAATAAGGTTGTTCAAACAGAT
GAAACTGCAAGGAAACCAGACCATGTTCCTATTAGCAGTGAAGATGAGAGGAATGCAATTTTCCAACTA
GAAAAGGCTATTTTATCTAATGAAGCCACCAAAAGTGACCTTCAGATGGCAGTGCTTTCATTTGAAAAA
GATGATGATCATAATGGACACATAGATTTCATCACAGCTGCATCAAATCTTCGTGCCAAAATGTACAGC
ATTGAACCAGCTGACCGTTTCAAAACAAAGCGCATAGCTGGTAAAATTATACCTGCTATAGCAACAACC
ACTGCTACAGTTTCTGGCTTGGTTGCCTTGGAGATGATCAAAGTAACTGGTGGCTATCCATTTGAAGCT
TACAAAAATTGTTTTCTTAACTTAGCCATTCCAATTGTAGTATTTACAGAGACAACTGAAGTAAGGAAA
ACTAAAATCAGAAATGGAATATCATTTACAATTTGGGATCGATGGACCGTACATGGAAAAGAAGATTTC
ACCCTCTTGGATTTCATAAATGCAGTCAAAGAGAAGTATGGAATTGAGCCAACAATGGTGGTACAGGGA
GTCAAAATGCTTTATGTTCCTGTAATGCCTGGTCATGCAAAAAGATTGAAGTTAACAATGCATAAACTT
GTAAAACCTACTACTGAAAAGAAATATGTGGATCTTACTGTGTCATTTGCTCCAGACATTGATGGAGAT
GAAGATTTGCCGGGACCTCCAGTAAGATACTACTTCAGTCATGACACTGATTAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_018227
Insert Size 3159 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018227.5
RefSeq Size 6462 bp
RefSeq ORF 3159 bp
Locus ID 55236
UniProt ID A0AVT1
Cytogenetics 4q13.2
Domains ThiF, UBACT
Protein Pathways Ubiquitin mediated proteolysis
MW 118 kDa
Summary Modification of proteins with ubiquitin (UBB; MIM 191339) or ubiquitin-like proteins controls many signaling networks and requires a ubiquitin-activating enzyme (E1), a ubiquitin conjugating enzyme (E2), and a ubiquitin protein ligase (E3). UBE1L2 is an E1 enzyme that initiates the activation and conjugation of ubiquitin-like proteins (Jin et al., 2007 PubMed 17597759).supplied by OMIM, Mar 2008
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"NM_018227" in other vectors (7)
SKU Description Size Price
RC214483 UBA6 (Myc-DDK-tagged)-Human ubiquitin-like modifier activating enzyme 6 (UBA6) 10 ug
$916.00
RC214483L1 Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 6 (UBA6), Myc-DDK-tagged 10 ug
$1,216.00
RC214483L2 Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 6 (UBA6), mGFP tagged 10 ug
$1,216.00
RC214483L3 Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 6 (UBA6), Myc-DDK-tagged 10 ug
$1,216.00
RC214483L4 Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 6 (UBA6), mGFP tagged 10 ug
$1,216.00
RG214483 UBA6 (tGFP-tagged) - Human ubiquitin-like modifier activating enzyme 6 (UBA6) 10 ug
$789.00 $1,116.00
SC111323 UBA6 (untagged)-Human ubiquitin-like modifier activating enzyme 6 (UBA6) 10 ug
$1,778.00

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