GRID2 (NM_001510) Human Tagged ORF Clone
$877.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | GRID2 |
| Synonyms | GluD2; SCAR18 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001510 |
| ORF Size | 3021 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001510.4 |
| RefSeq Size | 3024 bp |
| RefSeq ORF | 3024 bp |
| Locus ID | 2895 |
| UniProt ID | O43424 |
| Cytogenetics | 4q22.1-q22.2 |
| Protein Families | Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane |
| Protein Pathways | Long-term depression, Neuroactive ligand-receptor interaction |
| MW | 113.2 kDa |
| Summary | The protein encoded by this gene is a member of the family of ionotropic glutamate receptors which are the predominant excitatory neurotransmitter receptors in the mammalian brain. The encoded protein is a multi-pass membrane protein that is expressed selectively in cerebellar Purkinje cells. A point mutation in the mouse ortholog, associated with the phenotype named 'lurcher', in the heterozygous state leads to ataxia resulting from selective, cell-autonomous apoptosis of cerebellar Purkinje cells during postnatal development. Mice homozygous for this mutation die shortly after birth from massive loss of mid- and hindbrain neurons during late embryogenesis. This protein also plays a role in synapse organization between parallel fibers and Purkinje cells. Alternate splicing results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause cerebellar ataxia in humans. provided by RefSeq, Apr 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001510" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC214449L3 | Lenti-ORF clone of GRID2 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, delta 2 (GRID2) | 10 ug |
$1,177.00
|
|
| RC214449L4 | Lenti-ORF clone of GRID2 (mGFP-tagged)-Human glutamate receptor, ionotropic, delta 2 (GRID2) | 10 ug |
$1,177.00
|
|
| RG214449 | GRID2 (tGFP-tagged) - Human glutamate receptor, ionotropic, delta 2 (GRID2) | 10 ug |
$1,077.00
|
|
| SC123987 | GRID2 (untagged)-Human glutamate receptor, ionotropic, delta 2 (GRID2) | 10 ug |
$877.00
|
|
Citations
Absence of GluD2 Antibodies in Patients With Opsoclonus-Myoclonus Syndrome
Petit-Pedrol, Guasp, Armangue et al
Neurology (2021) 96 (7), e1082-e1087 DOI: 10.1212/WNL.0000000000011410
Petit-Pedrol, Guasp, Armangue et al
Neurology (2021) 96 (7), e1082-e1087 DOI: 10.1212/WNL.0000000000011410
AI Summary: OriGene’s GluD2 plasmids (RC210727 and RC210727L4) were used in cell-based assays to express GluD2 and test patient sera for GluD2 antibodies, helping demonstrate that such antibodies are absent in opsoclonus-myoclonus syndrome patients.
Absence of GluD2 Antibodies in Patients with Opsoclonus-Myoclonus Syndrome
M, M, T et al
Neurology (2020)
M, M, T et al
Neurology (2020)
AI Summary: OriGene’s GluD2 plasmid (Catalog number: RC214449) was used in cell-based assays to express human GluD2 and assess the presence of GluD2 antibodies in patient and control sera in this study.
Snippet: RC214449, Origene), and (2) a custom made plasmid with the human GluD2 Control adults 4/82 (5%) 6/82 (7%) 7/82 (8.5%) *Plasmid 1 = GluD2 clone with a Myc tag at the C-terminal (Accession number: NM_001510, Asp24-Ile1007;Catalog number RC214449, Origene;- RC214449, Origene), and (2) a custom made plasmid with the human GluD2 Control adults 4/82 (5%) 6/82 (7%) 7/82 (8.5%) *Plasmid 1 = GluD2 clone with a Myc tag at the C-terminal (Accession number: NM_001510, Asp24-Ile1007
Showing 1-2 of 2 papers.
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