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GRID2 (NM_001510) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC214449L4
Lenti-ORF clone of GRID2 (mGFP-tagged)-Human glutamate receptor, ionotropic, delta 2 (GRID2)
ORF Plasmid:
mGFP DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $1,177.00
3 Weeks*
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Other products for "GRID2 or NM_001510"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GRID2
Synonyms GluD2; SCAR18
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC214449).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001510
ORF Size 3021 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001510.2
RefSeq Size 3024 bp
RefSeq ORF 3024 bp
Locus ID 2895
UniProt ID O43424
Cytogenetics 4q22.1-q22.2
Protein Families Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
Protein Pathways Long-term depression, Neuroactive ligand-receptor interaction
MW 113.2 kDa
Summary The protein encoded by this gene is a member of the family of ionotropic glutamate receptors which are the predominant excitatory neurotransmitter receptors in the mammalian brain. The encoded protein is a multi-pass membrane protein that is expressed selectively in cerebellar Purkinje cells. A point mutation in the mouse ortholog, associated with the phenotype named 'lurcher', in the heterozygous state leads to ataxia resulting from selective, cell-autonomous apoptosis of cerebellar Purkinje cells during postnatal development. Mice homozygous for this mutation die shortly after birth from massive loss of mid- and hindbrain neurons during late embryogenesis. This protein also plays a role in synapse organization between parallel fibers and Purkinje cells. Alternate splicing results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause cerebellar ataxia in humans. provided by RefSeq, Apr 2014
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"NM_001510" in other vectors (4)
SKU Description Size Price
RC214449 GRID2 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, delta 2 (GRID2) 10 ug
$877.00
RC214449L3 Lenti-ORF clone of GRID2 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, delta 2 (GRID2) 10 ug
$1,177.00
RG214449 GRID2 (tGFP-tagged) - Human glutamate receptor, ionotropic, delta 2 (GRID2) 10 ug
$1,077.00
SC123987 GRID2 (untagged)-Human glutamate receptor, ionotropic, delta 2 (GRID2) 10 ug
$877.00

Plasmid Map for RC214449L4

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Cloning scheme for RC214449L4
Circular map for RC214449L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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