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CREB5 (NM_182898) Human Tagged ORF Clone

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SKU
RC213568
CREB5 (Myc-DDK-tagged)-Human cAMP responsive element binding protein 5 (CREB5), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $473.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CREB5
Synonyms CRE-BPA; CREB-5; CREBPA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213568 representing NM_182898
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTTATGAGGAATCCAAGATGAATTTGGAGCAGGAGAGGCCGTTTGTCTGCAGTGCCCCAGGCTGCT
CCCAGCGCTTCCCAACAGAGGACCATCTGATGATTCATAGGCACAAACATGAAATGACTTTGAAGTTTCC
TTCAATAAAAACAGACAATATGTTATCAGATCAAACTCCGACCCCAACGAGATTCCTGAAGAACTGCGAG
GAGGTGGGCCTCTTCAGCGAGCTGGACTGCTCCCTGGAGCACGAGTTCAGGAAGGCTCAGGAAGAGGAGA
GCAGCAAGCGGAATATCTCGATGCATAATGCAGTTGGTGGGGCCATGACGGGGCCCGGAACTCACCAGCT
TAGCAGCGCTCGGCTGCCCAACCATGACACCAACGTTGTGATTCAGCAAGCCATGCCGTCGCCTCAGTCC
AGCTCTGTCATCACTCAGGCACCTTCCACCAACCGCCAGATCGGGCCTGTCCCAGGCTCTCTATCTTCTC
TGCTACATCTCCACAACAGACAGAGACAGCCCATGCCAGCCTCCATGCCTGGGACCCTGCCCAACCCTAC
AATGCCAGGATCTTCCGCCGTCTTGATGCCAATGGAGCGACAAATGTCAGTGAACTCCAGCATCATGGGG
ATGCAAGGTCCAAATCTCAGCAACCCCTGTGCTTCTCCCCAGGTCCAGCCAATGCATTCAGAAGCCAAAA
TGAGGTTGAAGGCTGCATTGACTCACCACCCTGCTGCCATGTCAAATGGGAACATGAACACCATGGGACA
CATGATGGAGATGATGGGCTCCCGGCAGGACCAGACGCCACACCATCACATGCACTCGCACCCGCATCAG
CACCAGACACTGCCACCCCATCACCCTTACCCACACCAGCACCAGCACCCAGCACACCATCCTCACCCTC
AACCCCATCACCAGCAGAACCATCCACATCACCACTCCCATTCCCACCTTCATGCACACCCAGCACATCA
CCAGACCTCGCCACATCCGCCCCTGCACACCGGCAACCAAGCACAGGTTTCACCAGCAACACAACAGATG
CAGCCAACCCAGACAATACAGCCACCCCAGCCCACAGGGGGGCGCCGGCGAAGGGTGGTAGACGAGGATC
CGGACGAGAGGCGGCGGAAATTTCTGGAACGGAACCGGGCAGCTGCCACCCGCTGCAGACAGAAGAGGAA
GGTCTGGGTGATGTCATTGGAAAAGAAAGCAGAAGAACTCACCCAGACAAACATGCAGCTTCAGAATGAA
GTGTCTATGTTGAAAAATGAGGTGGCCCAGCTGAAACAGTTGTTGTTAACACATAAAGACTGCCCAATAA
CAGCCATGCAGAAAGAATCACAAGGATATCTAAGTCCAGAGAGTAGCCCTCCTGCTAGTCCTGTCCCAGC
TTGCTCCCAGCAACAAGTCATCCAGCATAATACCATCACTACTTCCTCATCGGTCAGCGAGGTGGTAGGA
AGCTCCACCCTCAGCCAGCTCACCACTCACAGAACAGACCTGAATCCGATTCTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213568 representing NM_182898
Red=Cloning site Green=Tags(s)

MIYEESKMNLEQERPFVCSAPGCSQRFPTEDHLMIHRHKHEMTLKFPSIKTDNMLSDQTPTPTRFLKNCE
EVGLFSELDCSLEHEFRKAQEEESSKRNISMHNAVGGAMTGPGTHQLSSARLPNHDTNVVIQQAMPSPQS
SSVITQAPSTNRQIGPVPGSLSSLLHLHNRQRQPMPASMPGTLPNPTMPGSSAVLMPMERQMSVNSSIMG
MQGPNLSNPCASPQVQPMHSEAKMRLKAALTHHPAAMSNGNMNTMGHMMEMMGSRQDQTPHHHMHSHPHQ
HQTLPPHHPYPHQHQHPAHHPHPQPHHQQNHPHHHSHSHLHAHPAHHQTSPHPPLHTGNQAQVSPATQQM
QPTQTIQPPQPTGGRRRRVVDEDPDERRRKFLERNRAAATRCRQKRKVWVMSLEKKAEELTQTNMQLQNE
VSMLKNEVAQLKQLLLTHKDCPITAMQKESQGYLSPESSPPASPVPACSQQQVIQHNTITTSSSVSEVVG
SSTLSQLTTHRTDLNPIL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_182898
ORF Size 1524 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182898.4
RefSeq Size 8532 bp
RefSeq ORF 1527 bp
Locus ID 9586
UniProt ID Q02930
Cytogenetics 7p15.1
Protein Families Transcription Factors
Protein Pathways Huntington's disease, Prostate cancer
MW 56.7 kDa
Summary The product of this gene belongs to the CRE (cAMP response element)-binding protein family. Members of this family contain zinc-finger and bZIP DNA-binding domains. The encoded protein specifically binds to CRE as a homodimer or a heterodimer with c-Jun or CRE-BP1, and functions as a CRE-dependent trans-activator. Alternatively spliced transcript variants encoding different isoforms have been identified. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_182898" in other vectors (4)
SKU Description Size Price
RC213568L3 Lenti-ORF clone of CREB5 (Myc-DDK-tagged)-Human cAMP responsive element binding protein 5 (CREB5), transcript variant 1 10 ug
$773.00
RC213568L4 Lenti-ORF clone of CREB5 (mGFP-tagged)-Human cAMP responsive element binding protein 5 (CREB5), transcript variant 1 10 ug
$773.00
RG213568 CREB5 (tGFP-tagged) - Human cAMP responsive element binding protein 5 (CREB5), transcript variant 1 10 ug
$673.00
SC309723 CREB5 (untagged)-Human cAMP responsive element binding protein 5 (CREB5), transcript variant 1 10 ug
$521.00

Plasmid Map for RC213568

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213568
Circular map for RC213568

file-alt Citations

RGS1 and CREB5 are direct and common transcriptional targets of ZNF384-fusion proteins
Yamada, Okada, Odaira et al
Cancer Med (2024) 13 (14), e7471 DOI: 10.1002/cam4.7471
Cited by 3
AI Summary: OriGene products RGS1/pCMV6-Entry (RC218112) and CREB5/pCMV6-Entry (RC213568) were used in this study to express RGS1 and CREB5 genes, enabling the authors to investigate their roles as direct transcriptional targets of ZNF384-fusion proteins in B-cell acute lymphoblastic leukemia.
Snippet: lag‐ZNF384/pcDNA at EcoRI and NotI sites and inserted into Myc/pSG5 at the same sites to form Myc‐ZNF384/pSG5. RGS1/pCMV6‐Entry (RC218112) and CREB5/pCMV6‐Entry (RC213568) were purchased from ORIGENE (Rockville, MD, USA). Luciferase reporter genes, RGS1‐pr/pGL4 and CREB5‐pr/pGL4, were made by the insertion of the regions from −497 to −77 and from +6 to +174 from the transcription s
FigureFigureFigure
Showing 1-1 of 1 papers.
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