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DACT2 (NM_214462) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC213496
DACT2 (Myc-DDK-tagged)-Human dapper, antagonist of beta-catenin, homolog 2 (Xenopus laevis) (DACT2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $709.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DACT2
Synonyms bA503C24.7; C6orf116; DAPPER2; DPR2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213496 representing NM_214462
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGACGCCGGGCGGACCCCCGGGGTCCGCGGGCTGGGACCGCCGTAGGTTGGGCGCGAGGTTGCGCG
CGGCGTTCGCGGGGCTGCAGGAGCTGCAGGGGCTGCGAGCCACGCAGCAGGAGCGGGTACGGGGCGCCCT
GGCCCTGCAGCCCCCGCCCGCGCCCGCCGCGCCCTGCGGCCCCCACGGCCTCCACGGCCCCGAGCAGCAG
CTGGAGGCGGCGCTGGCCGCGCTGCAGGAGCAGCTGTCCCGGCTGAGACAACAGGACATCGGCCTGAAGA
CCCACCTGGACCAGCTGGACCTGCAGATTAGCAAGCTGCAGCTGGATGTGGGCACAGCCTCAGGGGAGGC
CCTGGACAGCGACAGCAGGCCCAGCTCAGGCTTTTACGAGATGAGCGACGGTGGATCCTGCTCCCTGTCC
ACGTCCTGTGCCTCCGTCTGCAGTGACCACATCTCTCCCTCGCTGGGCAGTTTGTTGCCTGTGGCCCAGG
CCCACAAGGCCAGGCCCAGCATGGGGGACTGGAGGCCCCGGTCGGTTGATGAGACTACTGTGCCAGCGTG
GAGACCCCAGGCTACCGAGGAGGGCGCCAGGCCCCCAGGGAGCGTGGAGGATGCAGGCCAGCCGTGGGGC
ACATTCTGGCCCAGGCCTGTGTCTACAGGTGATCTTGACAGAGCCCTGCCGGCGGACACGGGGCTCCAGA
AAGCCAGCGCGGACGCCGAGCTCCTCGGGCTCCTCTGCCAGGGGGTGGATATCCCGCTGCACGTGCCGGA
CCCCAAGTATCGGCAGGACCTGGTGTCCCAGGGCGGCAGGGAGGTGTACCCGTACCCCAGCCCCCTGCAC
GCCGTGGCTCTACAGAGCCCCCTGTTTGTCCTGACTAAGGAAACCCCACAGAGAGGTGGCCCCTCGTTCC
CTAGGGAGAGCCCCAGGGGCCCCGCAGGTCTGAACACCATCCAGACTGGGCCGGTCCTCGAGGCTGGCCC
GGCCAGGGCCAGAGCTTATATCGACAGGTTGCTGCATCTGTGGGGCCGGGAGACCCCAGCAAAGGGTAGC
GAGGGAGAACAGGGACCTCTAAGGCATGCAGCGTCCCCATCTCCACAGAGGCAGGGTGGCTGGAGTACAG
ACGGTGGAGGGCGACTGCTGGTCTTCGCCCCAGGGAGGGAGGACGAGGGAGGGCCAGCTCAGAGCAGGGG
TGCCGGCAGGGGCGGGCCCCAGCAGCAGGGATACATGCCCCTTGAGGGTCCCCAGCAGTCTGGCAGCCTT
CCAGAGGAGGGCTCCAAGCCCTCAAACAGCTGTGTCCTCAGGGAGACCATGGTGCAGGCTTCTCCCAGCT
CAAAGGCCCAGCAGACACCCTCAGCTCAGGACTATGGACGAGGCAACATCATATCCCCATCCAGGATGCT
GGACAAGAGCCCCTCACCGGCCTCTGGGCACTTTGCCCACCCATCCTTTGCTGCCAGCCTGAAAATGGGT
CCCCCCAAGAGCAAGGCTGAAAAAATCAAGAGAAGTCCCATGGACAAGGTGCTGAGGTTTGCAAGGCAGC
CGCTGCTTCTACTGGACAGGCCTGAGGGAGCCCATGCAGCCCCCCAGCCATCCCTGGAGTGGGACCCTGC
CCACTGGCCCACAGGGAGGGGCGGGCTCCAGCGGAGGCCAGCCCTGGCCTGGGAGGCACCCGGGCGCTCC
TGTTCTGAGTCCACCCTCTACCCCATGCCTGTCCTCGTCCCCTTGGCAGTGGCCCCGCAGGAGAGCCACC
GGACCTCAGCCCAAGCCCTGTTCCCCTTTGAGGCGTCACTGCTCACCTCAGTGGCCAGGAGGAAGCATCG
CCGCTGGCAGTCCACCGTGGAGATCTCGGCCCGGGCCCGCCTGGCCAGCTGTCCTGAGTCTAACCTGGGG
CCCCCCAGGCCCGTGGCCAGGAGAGCAGGTGGCCCACTGGCCCGGGGCCGTCCCTCACTGGTCCGCCAGG
ACGCCTACACCAGGAGCGACTCAGAGCCCTCCAAGCACTCGGCCGAGTGTGACCCGCGGTTCCCGTCAGT
CATCCCGGAGACCAGCGAGGGAGAGTCCAGTGACCACACCACCAACCGATTCGGAGACCGTGAGTCCAGC
AGCAGCGACGAGGAGGGCGGCGCCCAGAGCAGGGACTGTGACCTGGCACTGGGCTATGTGGCGGCCGGGC
ATGCGGAGCTGGCCTGGACCCAGGAGGCCCCGGTCAGCTCGGGGCCACTCCTGTCCCCCGTGCCCAAGCT
GTGCCGTATTAAGGCCTCCAAGGCCCTGAAGAAGAAGATCCGCAGGTTCCAGCCGACGGCCCTGAAGGTC
ATGACCATGGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213496 representing NM_214462
Red=Cloning site Green=Tags(s)

MWTPGGPPGSAGWDRRRLGARLRAAFAGLQELQGLRATQQERVRGALALQPPPAPAAPCGPHGLHGPEQQ
LEAALAALQEQLSRLRQQDIGLKTHLDQLDLQISKLQLDVGTASGEALDSDSRPSSGFYEMSDGGSCSLS
TSCASVCSDHISPSLGSLLPVAQAHKARPSMGDWRPRSVDETTVPAWRPQATEEGARPPGSVEDAGQPWG
TFWPRPVSTGDLDRALPADTGLQKASADAELLGLLCQGVDIPLHVPDPKYRQDLVSQGGREVYPYPSPLH
AVALQSPLFVLTKETPQRGGPSFPRESPRGPAGLNTIQTGPVLEAGPARARAYIDRLLHLWGRETPAKGS
EGEQGPLRHAASPSPQRQGGWSTDGGGRLLVFAPGREDEGGPAQSRGAGRGGPQQQGYMPLEGPQQSGSL
PEEGSKPSNSCVLRETMVQASPSSKAQQTPSAQDYGRGNIISPSRMLDKSPSPASGHFAHPSFAASLKMG
PPKSKAEKIKRSPMDKVLRFARQPLLLLDRPEGAHAAPQPSLEWDPAHWPTGRGGLQRRPALAWEAPGRS
CSESTLYPMPVLVPLAVAPQESHRTSAQALFPFEASLLTSVARRKHRRWQSTVEISARARLASCPESNLG
PPRPVARRAGGPLARGRPSLVRQDAYTRSDSEPSKHSAECDPRFPSVIPETSEGESSDHTTNRFGDRESS
SSDEEGGAQSRDCDLALGYVAAGHAELAWTQEAPVSSGPLLSPVPKLCRIKASKALKKKIRRFQPTALKV
MTMV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_214462
ORF Size 2322 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_214462.5
RefSeq Size 2992 bp
RefSeq ORF 2325 bp
Locus ID 168002
UniProt ID Q5SW24
Cytogenetics 6q27
MW 83.1 kDa
Summary Involved in regulation of intracellular signaling pathways during development. Negatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors, such as TGFBR1. May be involved in control of the morphogenetic behavior of kidney ureteric bud cells by keeping cells epithelial and restraining their mesenchymal character. May play an inhibitory role in the re-epithelialization of skin wounds by attenuating TGF-beta signaling (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_214462" in other vectors (4)
SKU Description Size Price
RC213496L3 Lenti-ORF clone of DACT2 (Myc-DDK-tagged)-Human dapper, antagonist of beta-catenin, homolog 2 (Xenopus laevis) (DACT2) 10 ug
$1,009.00
RC213496L4 Lenti-ORF clone of DACT2 (mGFP-tagged)-Human dapper, antagonist of beta-catenin, homolog 2 (Xenopus laevis) (DACT2) 10 ug
$1,009.00
RG213496 DACT2 (tGFP-tagged) - Human dapper, antagonist of beta-catenin, homolog 2 (Xenopus laevis) (DACT2) 10 ug
$489.00 $909.00
SC318256 DACT2 (untagged)-Human dapper, antagonist of beta-catenin, homolog 2 (Xenopus laevis) (DACT2) 10 ug
$781.00

Plasmid Map for RC213496

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213496
Circular map for RC213496

file-alt Citations

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