DACT2 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | IF, IHC, WB |
|---|---|
| Recommended Dilution | WB: 1 ug/mL, ICC: 2.5 ug/mL, IF: 20 ug/mL |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Dact2 antibody was raised against a 12 amino acid peptide from near the amino terminus of human DACT2. |
| Buffer | PBS containing 0.02% sodium azide. |
| Purification | Affinity chromatography purified via peptide column |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | dishevelled binding antagonist of beta catenin 2 |
| Database Link | |
| Background | The Wnt signaling cascade is a conserved process in multicellular animals that plays important roles during development and can contribute to cancer and other diseases. Many members of this pathway are also expressed in the postnatal tissues such as brain. One such protein is Dact2, a member of the Dact protein family that was initially identified through binding to Disheveled (Dvl), a cytoplasmic protein essential to Wnt signaling. Dact2 is most prominent during the development of the thymus kidneys, and salivary gland. Dact2 is thought to play a role distinct from that of Dact1 with Dact2 having a greater impact on a b-catenin-independent process termed planar cell polarity/convergent-extension signaling. Furthermore, Dact2 but not Dact1 can inhibit Nodal signaling by promoting the endocytic degradation of TGF-b receptors. At least two isoforms of Dact2 are known to exist. |
| Synonyms | bA503C24.7; C6orf116; DAPPER2; DPR2 |
| Reference Data | |
| Protein Categories | Intracellular Proteins |
Reviews
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Citations
DACT2 regulates structural and electrical atrial remodeling in atrial fibrillation
Hou, Huang, Long et al
J Thorac Dis (2020) 12 (5), 2039-2048 DOI: 10.21037/jtd-19-4206
Hou, Huang, Long et al
J Thorac Dis (2020) 12 (5), 2039-2048 DOI: 10.21037/jtd-19-4206
Cited by 13
AI Summary: OriGene’s rabbit polyclonal DACT2 antibody was used for western blotting to assess DACT2 protein expression and investigate its role in atrial remodeling in atrial fibrillation.
Snippet: Antibody to, Mono/polyclonal, Company, Application (dilution). DACT2, Rabbit/poly, Abcam, Immunohistochemical staining (1:100). DACT2, Rabbit/poly, Origene, Western blotting (1:1,000). β-catenin, Rabbit/poly, Cell Signal Technology, Western blotting (1:1,000)
Downregulation of DACT-2 by Promoter Methylation and its Clinicopathological Significance in Prostate Cancer
Li, Yin, Huang et al
J Cancer (2019) 10 (7), 1755-1763 DOI: 10.7150/jca.28577
Li, Yin, Huang et al
J Cancer (2019) 10 (7), 1755-1763 DOI: 10.7150/jca.28577
Cited by 9
AI Summary: OriGene products contributed to this study by providing the DACT-2 cDNA clone (Cat# RC204307) for ectopic expression experiments, enabling the researchers to assess the effects of DACT-2 restoration on prostate cancer cell migration and invasion.
The new 6q27 tumor suppressor DACT2, frequently silenced by CpG methylation, sensitizes nasopharyngeal cancer cells to paclitaxel and 5-FU toxicity via β-catenin/Cdc25c signaling and G2/M arrest
Zhang, Fan, Fan et al
Clin Epigenet (2018) 10 (1) DOI: 10.1186/s13148-018-0459-2
Zhang, Fan, Fan et al
Clin Epigenet (2018) 10 (1) DOI: 10.1186/s13148-018-0459-2
AI Summary: OriGene products contributed to this paper by providing the DACT2 expression plasmid (RC210307) and DACT2-specific antibody (TA500201), which were used to restore DACT2 expression and detect its protein levels, respectively, enabling the study of DACT2’s role in cell cycle arrest and β-catenin/Cdc25c signaling in nasopharyngeal cancer cells.
Snippet: ted with _DACT2_ gene. The restoration of DACT2 expression inhibited NPC cell proliferation, migration, and invasiveness and induced G2/M cell cycle arrest. The Wnt signaling pathway is active in tumorigenesis, cell differentiation, and cell proliferation [25]. β-catenin is a transcription cofactor that induces target gene expression by binding to T cell factor/lymphoid enhancer factor (TCF/LEF)
The new 6q27 tumor suppressor DACT2, frequently silenced by CpG methylation, sensitizes nasopharyngeal cancer cells to paclitaxel and 5-FU toxicity via β-catenin/Cdc25c signaling and G2/M arrest
Zhang, Fan, Fan et al
Clin Epigenetics (2018) 10 (1), 26 DOI: 10.1186/s13148-018-0459-2
Zhang, Fan, Fan et al
Clin Epigenetics (2018) 10 (1), 26 DOI: 10.1186/s13148-018-0459-2
Cited by 46
AI Summary: OriGene products contributed to this study by providing the DACT2 expression plasmid (RC208307) and DACT2 antibody (TA500627), which were used for ectopic expression and protein detection of DACT2 in nasopharyngeal cancer cell experiments.
Upregulation of DACT2 suppresses proliferation and enhances apoptosis of glioma cell via inactivation of YAP signaling pathway
Tan, Li, Huang et al
Cell Death Dis (2017) 8 (8), e2981 DOI: 10.1038/cddis.2017.385
Tan, Li, Huang et al
Cell Death Dis (2017) 8 (8), e2981 DOI: 10.1038/cddis.2017.385
AI Summary: OriGene contributed to this study by providing the anti-DACT2 antibody, which was used to assess DACT2 protein expression in glioma cells and tissues.
Snippet: with 100U/ml penicillin, 100 µg/ml streptomycin (Hyclone) and 10% FBS. Antibodies. against DACT2 were obtained from OriGene, phospho-YAP, YAP, p-β-catenin,. β-catenin, p-LATS1, LATS1, PCNA, Bax, and CyclinD1 were obtained from Cell. ... All 2 versions Cite SaveSaving
Methylation of DACT2 promotes breast cancer development by activating Wnt signaling
Li, Zhang, He et al
Sci Rep (2017) 7 (1), 3325 DOI: 10.1038/s41598-017-03647-3
Li, Zhang, He et al
Sci Rep (2017) 7 (1), 3325 DOI: 10.1038/s41598-017-03647-3
AI Summary: OriGene’s DACT2 antibody (Cat: TA306668) was used for immunohistochemistry to detect DACT2 expression in primary breast cancer and adjacent tissue samples, enabling the study of its methylation status and role in breast cancer development.
Snippet: Immunohistochemistry (IHC) was performed in primary breast cancer samples and matched adjacent tissue samples. The DACT2 antibody was diluted to 1:500 (Cat: TA306668, OriGene Tech., MD, USA). ... Primary antibodies are as follows: DACT2 (OriGene Tech.
Showing 1-6 of 10 papers.
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