HSP90AA1 (NM_005348) Human Tagged ORF Clone
SKU
RC212496
HSP90AA1 (Myc-DDK-tagged)-Human heat shock protein 90kDa alpha (cytosolic), class A member 1 (HSP90AA1), transcript variant 2
$670.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | HSP90AA1 |
| Synonyms | EL52; HEL-S-65p; HSP86; Hsp89; HSP89A; Hsp90; HSP90A; HSP90N; Hsp103; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; LAP-2; LAP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005348 |
| ORF Size | 2196 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005348.4 |
| RefSeq Size | 2912 bp |
| RefSeq ORF | 2199 bp |
| Locus ID | 3320 |
| UniProt ID | P07900 |
| Cytogenetics | 14q32.31 |
| Domains | HATPase_c, HSP90 |
| Protein Families | Druggable Genome |
| Protein Pathways | Antigen processing and presentation, NOD-like receptor signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer |
| MW | 84.5 kDa |
| Summary | The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jan 2012 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005348" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC212496L1 | Lenti ORF clone of Human heat shock protein 90kDa alpha (cytosolic), class A member 1 (HSP90AA1), transcript variant 2, Myc-DDK-tagged | 10 ug |
$970.00
|
|
| RC212496L2 | Lenti ORF clone of Human heat shock protein 90kDa alpha (cytosolic), class A member 1 (HSP90AA1), transcript variant 2, mGFP tagged | 10 ug |
$970.00
|
|
| RC212496L3 | Lenti ORF clone of Human heat shock protein 90kDa alpha (cytosolic), class A member 1 (HSP90AA1), transcript variant 2, Myc-DDK-tagged | 10 ug |
$970.00
|
|
| RC212496L4 | Lenti ORF clone of Human heat shock protein 90kDa alpha (cytosolic), class A member 1 (HSP90AA1), transcript variant 2, mGFP tagged | 10 ug |
$970.00
|
|
| RG212496 | HSP90AA1 (tGFP-tagged) - Human heat shock protein 90kDa alpha (cytosolic), class A member 1 (HSP90AA1), transcript variant 2 | 10 ug |
$789.00
$870.00
|
|
| SC112678 | HSP90AA1 (untagged)-Human heat shock protein 90kDa alpha (cytosolic), class A member 1 (HSP90AA1), transcript variant 2 | 10 ug |
$671.00
|
|
Citations
YWK-II/APLP2 inhibits TGF-β signaling by interfering with the TGFBR2-Hsp90 interaction
Tuersuntuoheti, Li, Teng et al
Biochim Biophys Acta Mol Cell Res (2023) 1870 (7), 119548 DOI: 10.1016/j.bbamcr.2023.119548
Tuersuntuoheti, Li, Teng et al
Biochim Biophys Acta Mol Cell Res (2023) 1870 (7), 119548 DOI: 10.1016/j.bbamcr.2023.119548
Cited by 3
AI Summary: OriGene products pCMV-APLP2 (RC226790), pCMV-TGFBR2 (RC219855), and pCMV-Hsp90 (RC212496) were used as source plasmids for the open reading frames of key genes studied, enabling the authors to investigate how APLP2 modulates TGF-β signaling by affecting TGFBR2 stability and its interaction with Hsp90.
Snippet: Transforming growth factor-β (TGF-β) regulates multiple cellular biological processes by activating TGF-β type I receptors (TGFBR1) and type II receptors (TGFBR2), and Save;The open reading frame of target genes purchased from Origene (pCMV-APLP2: RC226790, pCMV-TGFBR2: RC219855, pCMV-Hsp90: RC212496) were subcloned into the PCDH-Flag / pcDNA3-HA/ pcDNA6-V5 vector (invitrogen), respectively
Expression of Intestinal Stem Cell and Cancer Stem Cell Markers in Submucosal Invasion and Its Prognostic Significance in Gastric Cancers
Kim, Song, Jeong et al
PrePrint (2020)
Kim, Song, Jeong et al
PrePrint (2020)
Cited by 1
Snippet: Full-length cDNA encoding CDX2 cDNA (pCMV6-CDX2) was purchased from OriGene (Rockville, MD, USA). The cells were transfected with 2.5 μg of pCMV6 systems), and HSP90 (Origene, Rockville, MD, USA) for overnight at 4 °C After washing with TBS;- Full-length cDNA encoding CDX2 cDNA (pCMV6-CDX2) was purchased from OriGene (Rockville, MD, USA). …
MicroRNA-361-Mediated Inhibition of HSP90 Expression and EMT in Cervical Cancer Is Counteracted by Oncogenic lncRNA NEAT1
Xu, Dong, Xiong et al
Cells (2020) 9 (3) DOI: 10.3390/cells9030632
Xu, Dong, Xiong et al
Cells (2020) 9 (3) DOI: 10.3390/cells9030632
Cited by 60
AI Summary: OriGene contributed to this study by providing the HSP90AA1 expression vector and the corresponding empty control vector, which were used to investigate the functional role of HSP90 in cervical cancer cell EMT and invasion.
Snippet: Expression vector encoding HSP90AA1 and the corresponding empty control vector were obtained from OriGene (Rockville, MD, USA). Cell transfections were performed using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol Cached
A biosensor-based framework to measure latent proteostasis capacity
Wood, Ormsby, Radwan et al
Nat Commun (2018) 9 (1), 287 DOI: 10.1038/s41467-017-02562-5
Wood, Ormsby, Radwan et al
Nat Commun (2018) 9 (1), 287 DOI: 10.1038/s41467-017-02562-5
AI Summary: OriGene products pCMV6-AC BAG1, pCMV6-AC HSF1, and pCMV6-Entry myc-HSP70AA1 plasmids were used in this study to overexpress BAG1, HSF1, and HSP70AA1, enabling the authors to investigate how these proteins modulate proteostasis capacity using their biosensor-based framework.
Snippet: pCMV6-AC BAG1 (cat# SC319483), pCMV6-AC HSF1 (cat# SC321225) and pCMV6-Entry myc-HSP70AA1 (cat# RC212496) were purchased from Origene
Mutations in PIH1D3 Cause X-Linked Primary Ciliary Dyskinesia with Outer and Inner Dynein Arm Defects
Paff, Loges, Aprea et al
Am. J. Hum. Genet. (2016)
Paff, Loges, Aprea et al
Am. J. Hum. Genet. (2016)
AI Summary: OriGene provided human PIH1D3 and HSP90 cDNA clones that were used in this study to investigate the molecular interactions and functional roles of PIH1D3 in primary ciliary dyskinesia.
Snippet: Human PIH1D3 and HSP90 clones were purchased from Origene. All other cDNAs have been previously cloned by nested PCR from human bronchial epithelial cell cDNA (ScienCell) using KOD polymerase according to manufacturer’s directions as previously described
Showing 1-5 of 5 papers.
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