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PKIB (NM_181795) Human Tagged ORF Clone

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SKU
RC212372
PKIB (Myc-DDK-tagged)-Human protein kinase (cAMP-dependent, catalytic) inhibitor beta (PKIB), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "PKIB or NM_181795"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PKIB
Synonyms PRKACN2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC212372 representing NM_181795
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGACAGATTCATCAAAAATGACTGACGTGGAGTCTGGGGTCGCCAATTTTGCATCTTCAGCAAGGG
CAGGCCGCCGGAATGCCTTACCAGACATCCAGAGTTCAGCTGCCACAGACGGAACCTCAGATTTGCCCCT
CAAACTGGAGGCTCTCTCCGTGAAGGAAGATGCAAAAGAGAAAGATGAAAAAACAACACAAGACCAATTG
GAAAAGCCTCAAAATGAAGAAAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC212372 representing NM_181795
Red=Cloning site Green=Tags(s)

MRTDSSKMTDVESGVANFASSARAGRRNALPDIQSSAATDGTSDLPLKLEALSVKEDAKEKDEKTTQDQL
EKPQNEEK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181795
ORF Size 234 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181795.3
RefSeq Size 1909 bp
RefSeq ORF 237 bp
Locus ID 5570
UniProt ID Q9C010
Cytogenetics 6q22.31
Protein Families Druggable Genome
MW 8.3 kDa
Summary This gene encodes a member of the cAMP-dependent protein kinase inhibitor family. The encoded protein may play a role in the protein kinase A (PKA) pathway by interacting with the catalytic subunit of PKA, and overexpression of this gene may play a role in prostate cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Jul 2012
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"NM_181795" in other vectors (6)
SKU Description Size Price
RC212372L1 Lenti ORF clone of Human protein kinase (cAMP-dependent, catalytic) inhibitor beta (PKIB), transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC212372L2 Lenti ORF clone of Human protein kinase (cAMP-dependent, catalytic) inhibitor beta (PKIB), transcript variant 1, mGFP tagged 10 ug
$450.00
RC212372L3 Lenti ORF clone of Human protein kinase (cAMP-dependent, catalytic) inhibitor beta (PKIB), transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC212372L4 Lenti ORF clone of Human protein kinase (cAMP-dependent, catalytic) inhibitor beta (PKIB), transcript variant 1, mGFP tagged 10 ug
$450.00
RG212372 PKIB (tGFP-tagged) - Human protein kinase (cAMP-dependent, catalytic) inhibitor beta (PKIB), transcript variant 1 10 ug
$489.00
SC109615 PKIB (untagged)-Human protein kinase (cAMP-dependent, catalytic) inhibitor beta (PKIB), transcript variant 1 10 ug
$150.00

Plasmid Map for RC212372

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC212372
Circular map for RC212372

file-alt Citations

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