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MTRR (NM_024010) Human Tagged ORF Clone

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SKU
RC212032
MTRR (Myc-DDK-tagged)-Human 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (MTRR), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $664.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MTRR
Synonyms cblE; MSR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC212032 representing NM_024010
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGCTGCGTCAGTGCGCGCTGGCGCAAGGTTGGTGGAAGTCGCGTTGTGCAGTTTCACTGTTACAT
GCCTTGAAGTGATGAGGAGGTTTCTGTTACTATATGCTACACAGCAGGGACAGGCAAAGGCCATCGCAGA
AGAAATATGTGAGCAAGCTGTGGTACATGGATTTTCTGCAGATCTTCACTGTATTAGTGAATCCGATAAG
TATGACCTAAAAACCGAAACAGCTCCTCTTGTTGTTGTGGTTTCTACCACGGGCACCGGAGACCCACCCG
ACACAGCCCGCAAGTTTGTTAAGGAAATACAGAACCAAACACTGCCGGTTGATTTCTTTGCTCACCTGCG
GTATGGGTTACTGGGTCTCGGTGATTCAGAATACACCTACTTTTGCAATGGGGGGAAGATAATTGATAAA
CGACTTCAAGAGCTTGGAGCCCGGCATTTCTATGACACTGGACATGCAGATGACTGTGTAGGTTTAGAAC
TTGTGGTTGAGCCGTGGATTGCTGGACTCTGGCCAGCCCTCAGAAAGCATTTTAGGTCAAGCAGAGGACA
AGAGGAGATAAGTGGCGCACTCCCGGTGGCATCACCTGCATCCTTGAGGACAGACCTTGTGAAGTCAGAG
CTGCTACACATTGAATCTCAAGTCGAGCTTCTGAGATTCGATGATTCAGGAAGAAAGGATTCTGAGGTTT
TGAAGCAAAATGCAGTGAACAGCAACCAATCCAATGTTGTAATTGAAGACTTTGAGTCCTCACTTACCCG
TTCGGTACCCCCACTCTCACAAGCCTCTCTGAATATTCCTGGTTTACCCCCAGAATATTTACAGGTACAT
CTGCAGGAGTCTCTTGGCCAGGAGGAAAGCCAAGTATCTGTGACTTCAGCAGATCCAGTTTTTCAAGTGC
CAATTTCAAAGGCAGTTCAACTTACTACGAATGATGCCATAAAAACCACTCTGCTGGTAGAATTGGACAT
TTCAAATACAGACTTTTCCTATCAGCCTGGAGATGCCTTCAGCGTGATCTGCCCTAACAGTGATTCTGAG
GTACAAAGCCTACTCCAAAGACTGCAGCTTGAAGATAAAAGAGAGCACTGCGTCCTTTTGAAAATAAAGG
CAGACACAAAGAAGAAAGGAGCTACCTTACCCCAGCATATACCTGCGGGATGTTCTCTCCAGTTCATTTT
TACCTGGTGTCTTGAAATCCGAGCAATTCCTAAAAAGGCATTTTTGCGAGCCCTTGTGGACTATACCAGT
GACAGTGCTGAAAAGCGCAGGCTACAGGAGCTGTGCAGTAAACAAGGGGCAGCCGATTATAGCCGCTTTG
TACGAGATGCCTGTGCCTGCTTGTTGGATCTCCTCCTCGCTTTCCCTTCTTGCCAGCCACCACTCAGTCT
CCTGCTCGAACATCTTCCTAAACTTCAACCCAGACCATATTCGTGTGCAAGCTCAAGTTTATTTCACCCA
GGAAAGCTCCATTTTGTCTTCAACATTGTGGAATTTCTGTCTACTGCCACAACAGAGGTTCTGCGGAAGG
GAGTATGTACAGGCTGGCTGGCCTTGTTGGTTGCTTCAGTTCTTCAGCCAAACATACATGCATCCCATGA
AGACAGCGGGAAAGCCCTGGCTCCTAAGATATCCATCTCTCCTCGAACAACAAATTCTTTCCACTTACCA
GATGACCCCTCAATCCCCATCATAATGGTGGGTCCAGGAACCGGCATAGCCCCGTTTATTGGGTTCCTAC
AACATAGAGAGAAACTCCAAGAACAACACCCAGATGGAAATTTTGGAGCAATGTGGTTGTTTTTTGGCTG
CAGGCATAAGGATAGGGATTATCTATTCAGAAAAGAGCTCAGACATTTCCTTAAGCATGGGATCTTAACT
CATCTAAAGGTTTCCTTCTCAAGAGATGCTCCTGTTGGGGAGGAGGAAGCCCCAGCAAAGTATGTGCAAG
ACAACATCCAGCTTCATGGCCAGCAGGTGGCAAGAATCCTCCTCCAGGAGAACGGCCATATTTATGTGTG
TGGAGATGCAAAGAATATGGCCAAGGATGTACATGATGCCCTTGTGCAAATAATAAGCAAAGAGGTTGGA
GTTGAAAAACTAGAAGCAATGAAAACCCTGGCCACTTTAAAAGAAGAAAAACGCTACCTTCAGGATATTT
GGTCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC212032 representing NM_024010
Red=Cloning site Green=Tags(s)

MGAASVRAGARLVEVALCSFTVTCLEVMRRFLLLYATQQGQAKAIAEEICEQAVVHGFSADLHCISESDK
YDLKTETAPLVVVVSTTGTGDPPDTARKFVKEIQNQTLPVDFFAHLRYGLLGLGDSEYTYFCNGGKIIDK
RLQELGARHFYDTGHADDCVGLELVVEPWIAGLWPALRKHFRSSRGQEEISGALPVASPASLRTDLVKSE
LLHIESQVELLRFDDSGRKDSEVLKQNAVNSNQSNVVIEDFESSLTRSVPPLSQASLNIPGLPPEYLQVH
LQESLGQEESQVSVTSADPVFQVPISKAVQLTTNDAIKTTLLVELDISNTDFSYQPGDAFSVICPNSDSE
VQSLLQRLQLEDKREHCVLLKIKADTKKKGATLPQHIPAGCSLQFIFTWCLEIRAIPKKAFLRALVDYTS
DSAEKRRLQELCSKQGAADYSRFVRDACACLLDLLLAFPSCQPPLSLLLEHLPKLQPRPYSCASSSLFHP
GKLHFVFNIVEFLSTATTEVLRKGVCTGWLALLVASVLQPNIHASHEDSGKALAPKISISPRTTNSFHLP
DDPSIPIIMVGPGTGIAPFIGFLQHREKLQEQHPDGNFGAMWLFFGCRHKDRDYLFRKELRHFLKHGILT
HLKVSFSRDAPVGEEEAPAKYVQDNIQLHGQQVARILLQENGHIYVCGDAKNMAKDVHDALVQIISKEVG
VEKLEAMKTLATLKEEKRYLQDIWS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024010
ORF Size 2175 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024010.2
RefSeq Size 3291 bp
RefSeq ORF 2097 bp
Locus ID 4552
UniProt ID Q9UBK8, Q9UBK8-2
Cytogenetics 5p15.31
Domains FAD_binding_1, flavodoxin, NAD_binding_1
Protein Families Druggable Genome
MW 80.2 kDa
Summary This gene encodes a member of the ferredoxin-NADP(+) reductase (FNR) family of electron transferases. This protein functions in the synthesis of methionine by regenerating methionine synthase to a functional state. Because methionine synthesis requires methyl-group transfer by a folate donor, activity of the encoded enzyme is important for folate metabolism and cellular methylation. Mutations in this gene can cause homocystinuria-megaloblastic anemia, cbl E type. Alternative splicing of this gene results in multiple transcript variants. provided by RefSeq, Dec 2015
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"NM_024010" in other vectors (6)
SKU Description Size Price
RC212032L1 Lenti ORF clone of Human 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (MTRR), transcript variant 2, Myc-DDK-tagged 10 ug
$964.00
RC212032L2 Lenti ORF clone of Human 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (MTRR), transcript variant 2, mGFP tagged 10 ug
$964.00
RC212032L3 Lenti ORF clone of Human 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (MTRR), transcript variant 2, Myc-DDK-tagged 10 ug
$964.00
RC212032L4 Lenti ORF clone of Human 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (MTRR), transcript variant 2, mGFP tagged 10 ug
$964.00
RG212032 MTRR (tGFP-tagged) - Human 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (MTRR), transcript variant 2 10 ug
$789.00 $864.00
SC314547 MTRR (untagged)-Human 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (MTRR), transcript variant 2 10 ug
$665.00

Plasmid Map for RC212032

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC212032
Circular map for RC212032

file-alt Citations

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