MTRR (NM_024010) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC212032L3
Lenti ORF clone of Human 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (MTRR), transcript variant 2, Myc-DDK-tagged
  $964.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MTRR
Synonyms cblE; MSR
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC212032).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024010
ORF Size 2175 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024010.1
RefSeq Size 3291 bp
RefSeq ORF 2097 bp
Locus ID 4552
UniProt ID Q9UBK8
Cytogenetics 5p15.31
Domains FAD_binding_1, flavodoxin, NAD_binding_1
Protein Families Druggable Genome
MW 80.2 kDa
Summary This gene encodes a member of the ferredoxin-NADP(+) reductase (FNR) family of electron transferases. This protein functions in the synthesis of methionine by regenerating methionine synthase to a functional state. Because methionine synthesis requires methyl-group transfer by a folate donor, activity of the encoded enzyme is important for folate metabolism and cellular methylation. Mutations in this gene can cause homocystinuria-megaloblastic anemia, cbl E type. Alternative splicing of this gene results in multiple transcript variants. provided by RefSeq, Dec 2015
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Documents
Resources
"NM_024010" in other vectors (6)
SKU Description Size Price
RC212032 MTRR (Myc-DDK-tagged)-Human 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (MTRR), transcript variant 2 10 ug
$664.00
RC212032L1 Lenti ORF clone of Human 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (MTRR), transcript variant 2, Myc-DDK-tagged 10 ug
$964.00
RC212032L2 Lenti ORF clone of Human 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (MTRR), transcript variant 2, mGFP tagged 10 ug
$964.00
RC212032L4 Lenti ORF clone of Human 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (MTRR), transcript variant 2, mGFP tagged 10 ug
$964.00
RG212032 MTRR (tGFP-tagged) - Human 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (MTRR), transcript variant 2 10 ug
$789.00 $864.00
SC314547 MTRR (untagged)-Human 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (MTRR), transcript variant 2 10 ug
$665.00

Plasmid Map for RC212032L3

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Cloning scheme for RC212032L3

Circular map for RC212032L3

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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