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DUSP10 (NM_144729) Human Tagged ORF Clone

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SKU
RC211820
DUSP10 (Myc-DDK-tagged)-Human dual specificity phosphatase 10 (DUSP10), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DUSP10
Synonyms MKP-5; MKP5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211820 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCCGTCTCCTTTAGACGACAGGGTAGTAGTGGCACTATCTAGGCCCGTCCGACCTCAGGATCTCA
ACCTTTGTTTAGACTCTAGTTACCTTGGCTCTGCCAACCCAGGCAGTAACAGCCACCCTCCTGTCATCGC
CACCACCGTTGTGTCCCTCAAGGCTGCGAATCTGACGTATATGCCCTCATCCAGCGGCTCTGCCCGCTCG
CTGAATTGTGGATGCAGCAGTGCCAGCTGCTGCACTGTGGCAACCTACGACAAGGACAATCAGGCCCAAA
CCCAAGCCATTGCCGCTGGCACCACCACCACTGCCATCGGAACCTCTACCACCTGCCCTGCTAACCAGAT
GGTCAACAATAATGAGAATACAGGCTCTCTAAGTCCATCAAGTGGGGTGGGCAGCCCTGTGTCAGGGACC
CCCAAGCAGCTAGCCAGCATCAAAATAATCTACCCCAATGACTTGGCAAAGAAGATGACCAAATGCAGCA
AGAGTCACCTGCCGAGTCAGGGCCCTGTCATCATTGACTGCAGGCCCTTCATGGAGTACAACAAGAGTCA
CATCCAAGGAGCTGTCCACATTAACTGTGCCGATAAGATCAGCCGGCGGAGACTGCAGCAGGGCAAGATC
ACTGTCCTAGACTTGATTTCCTGTAGGGAAGGCAAGGACTCTTTCAAGAGGATCTTTTCCAAAGAAATTA
TAGTTTATGATGAGAATACCAATGAACCAAGCCGAGTGATGCCCTCCCAGCCACTTCACATAGTCCTCGA
GTCCCTGAAGAGAGAAGGCAAAGAACCTCTGGTGTTGAAAGGTGGACTTAGTAGTTTTAAGCAGAACCAT
GAAAACCTCTGTGACAACTCCCTCCAGCTCCAAGAGTGCCGGGAGGTGGGGGGCGGCGCATCCGCGGCCT
CGAGCTTGCTACCTCAGCCCATCCCCACCACCCCTGACATCGAGAACGCTGAGCTCACCCCCATCTTGCC
CTTCCTGTTCCTTGGCAATGAGCAGGATGCTCAGGACCTGGACACCATGCAGCGGCTGAACATCGGCTAC
GTCATCAACGTCACCACTCATCTTCCCCTCTACCACTATGAGAAAGGCCTGTTCAACTACAAGCGGCTGC
CAGCCACTGACAGCAACAAGCAGAACCTGCGGCAGTACTTTGAAGAGGCTTTTGAGTTCATTGAGGAAGC
TCACCAGTGTGGGAAGGGGCTTCTCATCCACTGCCAGGCTGGGGTGTCCCGCTCCGCCACCATCGTCATC
GCTTACTTGATGAAGCACACTCGGATGACCATGACTGATGCTTATAAATTTGTCAAAGGCAAACGACCAA
TTATCTCCCCAAACCTTAACTTCATGGGGCAGTTGCTAGAGTTCGAGGAAGACCTAAACAACGGTGTGAC
ACCGAGAATCCTTACACCAAAGCTGATGGGCGTGGAGACGGTTGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211820 protein sequence
Red=Cloning site Green=Tags(s)

MPPSPLDDRVVVALSRPVRPQDLNLCLDSSYLGSANPGSNSHPPVIATTVVSLKAANLTYMPSSSGSARS
LNCGCSSASCCTVATYDKDNQAQTQAIAAGTTTTAIGTSTTCPANQMVNNNENTGSLSPSSGVGSPVSGT
PKQLASIKIIYPNDLAKKMTKCSKSHLPSQGPVIIDCRPFMEYNKSHIQGAVHINCADKISRRRLQQGKI
TVLDLISCREGKDSFKRIFSKEIIVYDENTNEPSRVMPSQPLHIVLESLKREGKEPLVLKGGLSSFKQNH
ENLCDNSLQLQECREVGGGASAASSLLPQPIPTTPDIENAELTPILPFLFLGNEQDAQDLDTMQRLNIGY
VINVTTHLPLYHYEKGLFNYKRLPATDSNKQNLRQYFEEAFEFIEEAHQCGKGLLIHCQAGVSRSATIVI
AYLMKHTRMTMTDAYKFVKGKRPIISPNLNFMGQLLEFEEDLNNGVTPRILTPKLMGVETVV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144729
ORF Size 1446 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144729.1, NM_144729.2, NP_653330.1
RefSeq Size 1824 bp
RefSeq ORF 422 bp
Locus ID 11221
Cytogenetics 1q41
Domains DSPc
Protein Families Druggable Genome, Phosphatase
Protein Pathways MAPK signaling pathway
MW 52.6 kDa
Summary Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAP kinase superfamily, which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAP kinases, different tissue distribution and subcellular localization, and different modes of expression induction by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK. Alternative splicing results in multiple transcript variants. provided by RefSeq, Apr 2014
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Documents
Resources
"NM_144729" in other vectors (4)
SKU Description Size Price
RC211820L3 Lenti ORF clone of Human dual specificity phosphatase 10 (DUSP10), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC211820L4 Lenti ORF clone of Human dual specificity phosphatase 10 (DUSP10), transcript variant 3, mGFP tagged 10 ug
$757.00
RG211820 DUSP10 (tGFP-tagged) - Human dual specificity phosphatase 10 (DUSP10), transcript variant 3 10 ug
$489.00 $657.00
SC310672 DUSP10 (untagged)-Human dual specificity phosphatase 10 (DUSP10), transcript variant 3 10 ug
$165.00

Plasmid Map for RC211820

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211820
Circular map for RC211820

file-alt Citations

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