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PPFIBP1 (NM_177444) Human Tagged ORF Clone

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SKU
RC211655
PPFIBP1 (Myc-DDK-tagged)-Human PTPRF interacting protein, binding protein 1 (liprin beta 1) (PPFIBP1), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "PPFIBP1 or NM_177444"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PPFIBP1
Synonyms hSGT2; hSgt2p; L2; SGT2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211655 representing NM_177444
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGAGTGATGCAAGTGACATGTTGGCTGCAGCGTTGGAGCAGATGGATGGTATCATAGCAGGTTCTA
AGGCTCTGGAATATTCCAATGGGATTTTTGATTGCCAATCTCCCACCTCTCCATTCATGGGAAGTTTGCG
AGCTCTGCACCTTGTGGAAGACCTGCGTGGATTGTTAGAGATGATGGAAACAGATGAGAAAGAAGGCTTG
AGATGCCAGATCCCAGATTCAACAGCAGAAACGCTTGTTGAATGGCTTCAGAGTCAAATGACAAATGGAC
ACCTACCAGGGAACGGAGATGTGTATCAAGAAAGGCTGGCACGTTTAGAAAATGATAAAGAATCCCTCGT
TCTTCAGGTAAGTGTGTTAACAGACCAGGTGGAGGCTCAGGGAGAGAAGATTCGAGATTTGGAGTTTTGT
CTTGAAGAGCACAGAGAGAAGTTGAATGCCACAGAAGAAATGCTGCAGCAGGTATGTGCAGAGGCCAGAA
CCAAGATGGGATTTCCCTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211655 representing NM_177444
Red=Cloning site Green=Tags(s)

MMSDASDMLAAALEQMDGIIAGSKALEYSNGIFDCQSPTSPFMGSLRALHLVEDLRGLLEMMETDEKEGL
RCQIPDSTAETLVEWLQSQMTNGHLPGNGDVYQERLARLENDKESLVLQVSVLTDQVEAQGEKIRDLEFC
LEEHREKLNATEEMLQQVCAEARTKMGFPC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177444
ORF Size 510 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177444.1, NP_803193.1
RefSeq Size 971 bp
RefSeq ORF 3036 bp
Locus ID 8496
UniProt ID Q86W92
Cytogenetics 12p11.23-p11.22
MW 18.8 kDa
Summary The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. It has been proposed that liprins are multivalent proteins that form complex structures and act as scaffolds for the recruitment and anchoring of LAR family of tyrosine phosphatases. This protein was found to interact with S100A4, a calcium-binding protein related to tumor invasiveness and metastasis. In vitro experiment demonstrated that the interaction inhibited the phosphorylation of this protein by protein kinase C and protein kinase CK2. Alternatively spliced transcript variants encoding distinct isoforms have been reported. provided by RefSeq, Jul 2008
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"NM_177444" in other vectors (4)
SKU Description Size Price
RC211655L3 Lenti ORF clone of Human PTPRF interacting protein, binding protein 1 (liprin beta 1) (PPFIBP1), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC211655L4 Lenti ORF clone of Human PTPRF interacting protein, binding protein 1 (liprin beta 1) (PPFIBP1), transcript variant 2, mGFP tagged 10 ug
$600.00
RG211655 PPFIBP1 (tGFP-tagged) - Human PTPRF interacting protein, binding protein 1 (liprin beta 1) (PPFIBP1), transcript variant 2 10 ug
$489.00 $500.00
SC126646 PPFIBP1 (untagged)-Human PTPRF interacting protein, binding protein 1 (liprin beta 1) (PPFIBP1), transcript variant 2 10 ug
$300.00

Plasmid Map for RC211655

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211655
Circular map for RC211655

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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