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PPFIBP1 (NM_177444) Human Untagged Clone

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SKU
SC126646
PPFIBP1 (untagged)-Human PTPRF interacting protein, binding protein 1 (liprin beta 1) (PPFIBP1), transcript variant 2
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PPFIBP1
Synonyms hSGT2; hSgt2p; L2; SGT2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_177444, the custom clone sequence may differ by one or more nucleotides


ATGATGAGTGATGCAAGTGACATGTTGGCTGCAGCGTTGGAGCAGATGGATGGTATCATAGCAGGTTCTA
AGGCTCTGGAATATTCCAATGGGATTTTTGATTGCCAATCTCCCACCTCTCCATTCATGGGAAGTTTGCG
AGCTCTGCACCTTGTGGAAGACCTGCGTGGATTGTTAGAGATGATGGAAACAGATGAGAAAGAAGGCTTG
AGATGCCAGATCCCAGATTCAACAGCAGAAACGCTTGTTGAATGGCTTCAGAGTCAAATGACAAATGGAC
ACCTACCAGGGAACGGAGATGTGTATCAAGAAAGGCTGGCACGTTTAGAAAATGATAAAGAATCCCTCGT
TCTTCAGGTAAGTGTGTTAACAGACCAGGTGGAGGCTCAGGGAGAGAAGATTCGAGATTTGGAGTTTTGT
CTTGAAGAGCACAGAGAGAAGTTGAATGCCACAGAAGAAATGCTGCAGCAGGAGCTTCTAAGTAGGACAT
CCTTAGAAACTCAGAAGTTGGATCTGATGGCTGAAATATCTAACTTGAAGTTGAAACTGACAGCTGTAGA
GAAGGACAGATTGGATTATGAAGATAAGTTCAGAGACACAGAGGGGCTGATTCAGGAGATCAATGATTTG
AGGTTAAAAGTTAGTGAAATGGACAGTGAGAGACTTCAGTATGAAAAAAAGCTTAAATCAACCAAATCTT
TAATGGCCAAACTTTCTAGCATGAAAATCAAGGTGGGTCAGATGCAGTATGAAAAGCAGCGGATGGAACA
AAAATGGGAGTCACTGAAGGATGAACTGGCATCTTTAAAAGAACAACTAGAAGAAAAGGAATCTGAAGTA
AAAAGGCTACAAGAAAAATTGGTTTGCAAGATGAAAGGAGAAGGGGTTGAAATTGTTGATAGAGACATCG
AAGTACAAAAAATGAAAAAAGCTGTGGAGTCCTTGATGGCAGCAAATGAAGAAAAGGATCGGAAAATAGA
AGATCTTCGACAGTGCCTGAACAGGTACAAGAAAATGCAAGACACGGTGGTACTGGCCCAAGGCAAAGAT
GGAGAATATGAAGAGCTGCTCAATTCCAGTTCCATCTCCTCTTTGCTGGATGCACAGGGTTTCAGTGATC
TGGAGAAAAGTCCATCACCCACTCCAGTAATGGGATCTCCCAGTTGTGACCCATTTAACACAAGTGTTCC
CGAAGAGTTCCATACTACCATCTTGCAAGTTTCCATCCCTTCATTATTGCCAGCAACTGTAAGCATGGAA
ACTTCTGAAAAATCAAAGTTGACTCCTAAGCCAGAGACTTCATTTGAAGAAAATGATGGAAACATAATCC
TTGGTGCCACTGTTGATACCCAACTGTGTGATAAACTTTTAACTTCAAGTCTGCAGAAGTCCAGCAGCCT
GGGCAATCTGAAGAAAGAGACATCTGATGGGGAAAAGGAAACTATTCAGAAGACTTCAGAGGACAGAGCT
CCGGCAGAAAGCAGGCCATTTGGGACCCTTCCTCCCAGGCCCCCAGGGCAGGACACCTCCATGGATGACA
ACCCCTTCGGCACTCGAAAAGTCAGATCTTCCTTTGGCCGGGGCTTTTTTAAAATCAAAAGTAACAAGAG
AACAGCAAGTGCACCAAACTTAGCTGAAACAGAAAAAGAGACAGCAGAGCACCTAGATCTGGCTGGTGCT
TCTTCTCGGCCAAAAGATTCACAGAGGAACAGTCCCTTCCAGATACCGCCTCCATCTCCAGATTCCAAAA
AGAAATCCAGAGGTATCATGAAACTCTTTGGAAAACTTAGGAGAAGTCAATCAACTACATTCAACCCAGA
TGACATGTCTGAGCCTGAATTCAAAAGAGGAGGGACAAGGGCAACCGCGGGGCCCCGATTAGGTTGGTCT
CGAGACTTGGGACAGTCTAACAGTGACTTGGATATGCCATTTGCCAAGTGGACCAAGGAGCAGGTTTGCA
ATTGGCTGATGGAACAGGGCTTGGGCTCGTACCTGAATTCTGGCAAGCACTGGATTGCATCTGGCCAAAC
GCTTTTGCAGGCTTCTCAACAAGATCTAGAGAAGGAACTTGGAATCAAGCATTCACTTCATCGAAAGAAA
CTCCAGCTAGCACTCCAAGCCCTGGGATCTGAAGAAGAAACCAATCATGGGAAGCTGGATTTCAACTGGG
TCACTAGATGGTTGGATGACATTGGCCTCCCTCAATATAAGACCCAGTTTGATGAAGGACGGGTTGATGG
TCGAATGCTACATTACATGACTGTTGATGACTTACTGTCTCTGAAGGTTGTAAGTGTGCTACACCATCTC
AGTATCAAAAGGGCCATCCAGGTCCTGAGGATCAATAACTTTGAACCAAACTGTCTACGGAGGCGGCCAT
CTGATGAGAATACCATCGCCCCATCAGAAGTTCAGAAGTGGACTAACCATCGAGTGATGGAGTGGCTGCG
CTCCGTGGACTTGGCAGAATATGCGCCCAATCTCAGAGGCAGTGGTGTCCATGGTGGGCTCATGGTTCTA
GAGCCTCGTTTTAACGTAGAAACAATGGCTCAGTTATTGAACATCCCACCCAATAAGACTTTGCTGCGAA
GACATTTGGCCACTCATTTCAACCTTCTGATTGGGGCTGAGGCACAGCACCAGAAGCGAGATGCCATGGA
GCTGCCGGATTATGTACTTCTAACAGCTACTGCCAAAGTGAAGCCAAAGAAACTTGCCTTTAGCAATTTT
GGGAATTTGAGAAAGAAGAAACAGGAAGATGGTGAAGAATATGTTTGTCCAATGGAATTGGGACAGGCAT
CAGGAAGTGCATCTAAGAAAGGATTTAAACCTGGTTTGGATATGCGCCTGTATGAGGAAGATGATTTGGA
CCGGTTAGAGCAGATGGAAGATTCAGAAGGGACAGTGAGACAGATAGGTGCATTCTCTGAAGGCATCAAC
AATCTGACGCACATGTTAAAAGAAGATGACATGTTTAAAGATTTTGCTGCCCGTTCCCCCAGTGCCAGCA
TTACAGATGAAGACTCAAACGTTTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_177444 unedited
TGTAATACCGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTC
GGCACGAGGCCGGGNNNGAGTGCTGGGGCTTTGAACTCCGAGAGGAGGTGGACCAGAACT
TTTGGAACTAGTGCCGGCGGCTCTCCACCCCCCGNNNTAAAAGAACGTGTGGATCACTTT
GCTGAGTACATCCAAGATTTGAAGAACTGAAATAAATCAGCTTTAAACCNNCTTTTTAAA
AATATCTGGGTTGGAATTTGCCCCTGACAAATAATAAAATGATGAGTGATGCAAGTGACA
TGTNNNCTGCAGCGTTGGAGCAGATGGATGGTATCATAGCAGGTTCTAAGGCTCTGGAAT
ATTCCAATGGGATTTNNNATTGCCAATCTCCCACCTCTCCATTCATGGGAAGTTTGCGAG
CTCTGCACCTTGTGGAAGACCTGCGTGGATTNNNAGAGATGATGGAAACAGATGAGAAAG
AAGGCTTGAGATGCCAGATCCCAGATTCAACAGCAGAAACGCTTGTTGGNNNNCTTCAGA
GTCAAATGACAAATGGACACCTACCAGGGAACGGAGATGTGTATCAAGAAAGGCTGGCAC
GTTTAGAAAATGATAAAGNNNNNCTCGTTCTTAGGTAAGTGTGTTAACAGACCAGGTGGA
GGCTCAGGGAGAGAAGATTCGAGATTTGGAGNTTTTGTCTTGAAGAGCACANNNNNAGGT
TGAATGCCACAGAAGAAATGCTGCAGCGGGTATGTGCAGAGGCCANAACCAGATGGGATN
TCCCTGCTGAACTATGTGAGATGCTGCATTTCTATGGTGNNNNAAGTCTGATGCAGCAGT
AAGTTTCTTGATTCCCTTAGACCGCACAAAAAAAAAAAAAAACTCACNTTAGATTGCGGG
CGCGTCATAGCTGGTCCTGACAAATCCNGNTTGGCATCCTGTGNNNNNNNCAGTACCTNC
TGNCCTGNAAGTGCCCTNCANGCCC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_177444 unedited
ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTGCTGGTCTAAGTGAA
TCAAGAAACTTACTGCTGCATCAGAGCAAACACAACATAGAAATGCAGCATCTCACATAG
TTCAGCAGGGAAATCCCATCTTGGTTCTGGCCTCTGCACATACCTGCTGCAGCATTTCTT
CTGTGGCATTCAACTTCTCTCTGTGCTCTTCAAGACAAAACTCCAAATCTCGAATCTTCT
CTCCCTGAGCCTCCACCTGGTCTGTTAACACACTTACCTGAAGAACGAGGGATTCTTTAT
CATTTTCTAAACGTGCCAGCCTTTCTTGATACACATCTCCGTTCCCTGGTAGGTGTCCAT
TTGTCATTTGACTCTGAAGCCATTCAACAAGCGTTTCTGCTGTTGAATCTGGGATCTGGC
ATCTCAAGCCTTCTTTCTCATCTGTTTCCATCATCTCTAACAATCCACGCAGGTCTTCCA
CAAGGTGCAGAGCTCGCAAACTTCCCATGAATGGAGAGGTGGGAGATTGGCAATCAAAAA
TCCCATTGGAATATTCCAGAGCCTTAGAACCTGCTATGATACCATCCATCTGCTCCAACG
CTGCAGCCAACATGTCACTTGCATCACTCATCATTTTATTATTTGTCAGGGGCAAATTCC
AACCCAGATATTTTTAAAAAGCANGTTTAAAGCTGATTTATTTCAGTTCTTCAAATCTTG
GATGTACTCAGCAAAGTGATCCACACGTTCTTTTTACTGNGGGGTGGAGAGCCCGCGCAC
TAGTCCAAAAAGTCTGTCACCCTCTCTCGAGTCAAAGCCCACACTCCGNCCGGNCTCTGC
CGAATCGAACNNAGCCTCTGCCCGATCNCGNCGNCCTTANNGAGTCTATACAAAATCTGA
CGTACTAACAGCTTGCTTATAGACTCC
Restriction Sites NotI-NotI |
ACCN NM_177444
Insert Size 880 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177444.1, NP_803193.1
RefSeq Size 971 bp
RefSeq ORF 513 bp
Locus ID 8496
UniProt ID Q86W92
Cytogenetics 12p11.23-p11.22
Summary The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. It has been proposed that liprins are multivalent proteins that form complex structures and act as scaffolds for the recruitment and anchoring of LAR family of tyrosine phosphatases. This protein was found to interact with S100A4, a calcium-binding protein related to tumor invasiveness and metastasis. In vitro experiment demonstrated that the interaction inhibited the phosphorylation of this protein by protein kinase C and protein kinase CK2. Alternatively spliced transcript variants encoding distinct isoforms have been reported. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2).
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"NM_177444" in other vectors (4)
SKU Description Size Price
RC211655 PPFIBP1 (Myc-DDK-tagged)-Human PTPRF interacting protein, binding protein 1 (liprin beta 1) (PPFIBP1), transcript variant 2 10 ug
$300.00
RC211655L3 Lenti ORF clone of Human PTPRF interacting protein, binding protein 1 (liprin beta 1) (PPFIBP1), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC211655L4 Lenti ORF clone of Human PTPRF interacting protein, binding protein 1 (liprin beta 1) (PPFIBP1), transcript variant 2, mGFP tagged 10 ug
$600.00
RG211655 PPFIBP1 (tGFP-tagged) - Human PTPRF interacting protein, binding protein 1 (liprin beta 1) (PPFIBP1), transcript variant 2 10 ug
$489.00 $500.00

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