GNRH2 (NM_178331) Human Tagged ORF Clone
SKU
RC211474
GNRH2 (Myc-DDK-tagged)-Human gonadotropin-releasing hormone 2 (GNRH2), transcript variant 3
$150.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | GNRH2 |
| Synonyms | GnRH-II; LH-RHII |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_178331 |
| ORF Size | 339 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_178331.2 |
| RefSeq Size | 402 bp |
| RefSeq ORF | 342 bp |
| Locus ID | 2797 |
| UniProt ID | O43555 |
| Cytogenetics | 20p13 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | GnRH signaling pathway |
| MW | 9.8 kDa |
| Summary | This gene is a member of the gonadotropin-releasing hormone (GnRH) gene family. Proteins encoded by members of this gene family are proteolytically cleaved to form neuropeptides which, in part, regulate reproductive functions by stimulating the production and release of the gonadotropins follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The human GNRH2 gene is predicted to encode a preproprotein from which a mature neuropeptide of 10 amino acids is cleaved. However, while the human genome retains the sequence for a functional GNRH2 decapeptide, translation of the human GNRH2 gene has not yet been demonstrated and the GNRH2 gene of chimpanzees, gorilla, and Sumatran orangutan have a premature stop at codon eight of the decapeptide sequence which suggests GNRH2 was a pseudogene in the hominid lineage. The GNRH2 gene is also believed to be a pseudogene in many other mammalian species such as mouse and cow. The receptor for this gene (GNRHR2) is predicted to be a pseudogene in human as well as many other mammalian species. The closely related GNRH1 and GNRHR1 genes are functional in human and other mammals and are generally functional in vertebrates. provided by RefSeq, Mar 2019 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_178331" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC211474L3 | Lenti-ORF clone of GNRH2 (Myc-DDK-tagged)-Human gonadotropin-releasing hormone 2 (GNRH2), transcript variant 3 | 10 ug |
$450.00
|
|
| RC211474L4 | Lenti-ORF clone of GNRH2 (mGFP-tagged)-Human gonadotropin-releasing hormone 2 (GNRH2), transcript variant 3 | 10 ug |
$450.00
|
|
| RG211474 | GNRH2 (tGFP-tagged) - Human gonadotropin-releasing hormone 2 (GNRH2), transcript variant 3 | 10 ug |
$350.00
|
|
| SC307152 | GNRH2 (untagged)-Human gonadotropin-releasing hormone 2 (GNRH2), transcript variant 3 | 10 ug |
$165.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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