SERTAD2 (NM_014755) Human Tagged ORF Clone

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SKU
RC210964
SERTAD2 (Myc-DDK-tagged)-Human SERTA domain containing 2 (SERTAD2)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SERTAD2
Synonyms Sei-2; TRIP-Br2; TRIPBR2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210964 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGGGTAAAGGAGGAAAACGGAAGTTTGATGAGCATGAAGATGGGCTGGAAGGCAAAATCGTGTCTC
CCTGTGACGGTCCATCCAAGGTGTCTTACACCTTACAGCGCCAGACTATCTTCAACATTTCCCTTATGAA
ACTCTATAACCACAGGCCCCTGACAGAGCCCAGCTTGCAAAAGACCGTTTTAATTAACAACATGTTGAGG
CGGATCCAGGAGGAACTCAAACAGGAAGGCAGCCTGAGGCCCATGTTCACCCCCTCCTCCCAGCCCACCA
CCGAGCCCAGCGACAGCTACCGAGAGGCCCCGCCGGCCTTCAGCCACCTGGCGTCCCCGTCCTCCCACCC
CTGCGACCTCGGAAGCACTACGCCCCTGGAGGCCTGCCTCACCCCGGCCTCACTGCTCGAGGACGACGAT
GACACGTTTTGCACCTCCCAGGCCATGCAGCCCACGGCTCCCACCAAACTGTCACCTCCAGCCCTCTTGC
CAGAAAAGGACAGTTTCTCCTCTGCCTTGGACGAGATCGAGGAGCTCTGTCCCACATCTACCTCCACAGA
GGCGGCCACGGCTGCGACTGACAGTGTGAAAGGGACCTCCAGCGAGGCTGGCACCCAGAAACTCGACGGT
CCTCAAGAGAGCCGCGCAGATGACTCAAAACTGATGGACTCTCTGCCTGGGAATTTTGAAATAACGACGT
CCACGGGTTTCCTGACAGACTTGACCCTGGATGACATCCTGTTTGCTGACATTGATACGTCCATGTATGA
TTTTGACCCCTGCACTTCCTCATCAGGGACAGCCTCAAAAATGGCCCCTGTGTCTGCCGACGACCTCCTC
AAAACTCTGGCTCCTTACAGCAGTCAGCCTGTCACCCCAAGTCAGCCTTTCAAAATGGACCTCACAGAGC
TGGACCACATCATGGAGGTGCTTGTTGGGTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC210964 protein sequence
Red=Cloning site Green=Tags(s)

MLGKGGKRKFDEHEDGLEGKIVSPCDGPSKVSYTLQRQTIFNISLMKLYNHRPLTEPSLQKTVLINNMLR
RIQEELKQEGSLRPMFTPSSQPTTEPSDSYREAPPAFSHLASPSSHPCDLGSTTPLEACLTPASLLEDDD
DTFCTSQAMQPTAPTKLSPPALLPEKDSFSSALDEIEELCPTSTSTEAATAATDSVKGTSSEAGTQKLDG
PQESRADDSKLMDSLPGNFEITTSTGFLTDLTLDDILFADIDTSMYDFDPCTSSSGTASKMAPVSADDLL
KTLAPYSSQPVTPSQPFKMDLTELDHIMEVLVGS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014755
ORF Size 942 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014755.3
RefSeq Size 5565 bp
RefSeq ORF 945 bp
Locus ID 9792
UniProt ID Q14140
Cytogenetics 2p14
Protein Families Transcription Factors
MW 33.9 kDa
Summary Acts at E2F-responsive promoters as coregulator to integrate signals provided by PHD- and/or bromodomain-containing transcription factors. May act as coactivator as well as corepressor of E2F1-TFDP1 and E2F4-TFDP1 complexes on E2F consensus binding sites, which would activate or inhibit E2F-target genes expression. Modulates fat storage by down-regulating the expression of key genes involved in adipocyte lipolysis, thermogenesis and oxidative metabolism.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_014755" in other vectors (4)
SKU Description Size Price
RC210964L3 Lenti ORF clone of Human SERTA domain containing 2 (SERTAD2), Myc-DDK-tagged 10 ug
$600.00
RC210964L4 Lenti ORF clone of Human SERTA domain containing 2 (SERTAD2), mGFP tagged 10 ug
$600.00
RG210964 SERTAD2 (tGFP-tagged) - Human SERTA domain containing 2 (SERTAD2) 10 ug
$489.00 $500.00
SC108703 SERTAD2 (untagged)-Human SERTA domain containing 2 (SERTAD2) 10 ug
$300.00

Plasmid Map for RC210964

Download sequence for

Cloning scheme for RC210964

Circular map for RC210964

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