RNF122 (NM_024787) Human Tagged ORF Clone
$150.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | RNF122 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_024787 |
| ORF Size | 465 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_024787.3, NP_079063.2 |
| RefSeq Size | 1872 bp |
| RefSeq ORF | 468 bp |
| Locus ID | 79845 |
| UniProt ID | Q9H9V4 |
| Cytogenetics | 8p12 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 17.5 kDa |
| Summary | The encoded protein contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. The encoded protein is localized to the endoplasmic reticulum and golgi apparatus, and may be associated with cell viability. provided by RefSeq, Jul 2013 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_024787" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210868L3 | Lenti ORF clone of Human ring finger protein 122 (RNF122), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC210868L4 | Lenti ORF clone of Human ring finger protein 122 (RNF122), mGFP tagged | 10 ug |
$450.00
|
|
| RG210868 | RNF122 (tGFP-tagged) - Human ring finger protein 122 (RNF122) | 10 ug |
$489.00
|
|
| SC305152 | RNF122 (untagged)-Human ring finger protein 122 (RNF122) | 10 ug |
$165.00
|
|
Citations
The RNA-Binding Ubiquitin Ligase MEX3A Affects Glioblastoma Tumorigenesis by Inducing Ubiquitylation and Degradation of RIG-I
Bufalieri, Caimano, Lospinoso Severini et al
Cancers (Basel) (2020) 12 (2) DOI: 10.3390/cancers12020321
Bufalieri, Caimano, Lospinoso Severini et al
Cancers (Basel) (2020) 12 (2) DOI: 10.3390/cancers12020321
Cited by 68
AI Summary: OriGene products, specifically the shCTR TR30021 and shMEX3A TL308061B (#1) and TL308061C (#2) shRNA plasmids, were used to genetically deplete MEX3A in glioblastoma cells, enabling the study of its role in tumorigenesis.
Snippet: …In this study, the RNA-binding ubiquitin ligase MEX3A was selected from a gene expression analysis performed on publicly available datasets, to assess its biological and still-unknown activity in GB tumorigenesis. We find that MEX3A is strongly up-regulated in GB specimens, and this correlates with very low protein levels of RIG-I, a tumor suppressor involved in differentiation, apoptosis and innate immune response. We demonstrate that MEX3A binds RIG-I and induces its ubiquitylation and proteasome-dependent degradation. Further, the genetic depletion of MEX3A leads to an increase of RIG-I protein levels and results in the suppression of GB cell growth. Our findings unveil a novel molecular mechanism involved in GB tumorigenesis and suggest MEX3A and RIG-I as promising therapeutic targets in GB.;Lentiviral particles were generated in HEK293 cells transfected with packaging and envelope plasmids (pCMV-dR8.74 and VSV-G/pMDG2), pGFP-pLKO.1 plasmids (shCTR TR30021;shMEX3A TL308061B (#1), TL308061C (#2), Origene, Rockville, MD, USA)
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
