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NCKAP1L (NM_005337) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC210832
NCKAP1L (Myc-DDK-tagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $981.00
5 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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Other products for "NCKAP1L or NM_005337"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NCKAP1L
Synonyms HEM1; IMD72
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210832 representing NM_005337
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTTGACATCTGCTTACCAGCATAAATTAGCAGAGAAGCTCACTATCCTGAATGATCGCGGTCAGG
GGGTTCTCATCCGTATGTATAACATCAAGAAGACTTGTTCAGACCCCAAATCTAAGCCACCTTTCTTACT
GGAAAAGTCCATGGAACCATCTCTCAAGTATATCAACAAGAAATTTCCCAACATAGATGTCCGAAACAGC
ACGCAACATTTAGGACCAGTACATCGTGAAAAAGCCGAGATAATTAGATTCCTCACCAACTACTACCAGT
CATTTGTGGATGTCATGGAATTTCGGGATCATGTATATGAACTTCTCAACACCATTGATGCCTGCCAGTG
CCATTTTGATATCAATCTCAACTTTGATTTCACTCGGAGTTACCTGGACTTGATTGTAACTTACACCTCA
GTCATTTTACTTCTGTCACGGATTGAAGATCGGCGGATACTCATTGGCATGTACAATTGTGCCCATGAGA
TGCTGCATGGGCATGGTGACCCCAGTTTTGCCCGTCTGGGTCAGATGGTCTTGGAGTATGACCACCCTCT
GAAGAAGCTGACAGAAGAGTTTGGGCCTCACACAAAGGCTGTGAGTGGAGCCCTCCTCTCTTTGCATTTC
CTCTTTGTCCGAAGAAACCAGGGGGCTGAGCAGTGGCGCAGTGCCCAACTTCTAAGCCTCATCAGCAACC
CCCCAGCCATGATTAACCCTGCTAATTCAGATACAATGGCCTGTGAGTATCTGTCTGTGGAAGTAATGGA
GCGCTGGATTATCATTGGGTTTCTTCTTTGTCATGGGTGCCTCAACTCCAATAGCCAGTGCCAGAAGCTG
TGGAAGCTGTGTCTGCAGGGCTCCCTCTACATCACCCTTATCCGTGAGGATGTGCTGCAGGTGCACAAAG
TCACCGAGGACCTGTTTAGCAGTTTGAAAGGGTATGGCAAGAGAGTGGCAGACATAAAGGAGAGCAAGGA
ACATGTAATTGCAAACAGTGGCCAGTTTCATTGTCAACGGCGGCAATTTCTGCGGATGGCAGTGAAGGAG
CTGGAGACTGTGTTGGCTGATGAACCGGGACTACTGGGTCCTAAGGCTCTTTTTGCTTTCATGGCCCTGT
CCTTCATTCGTGATGAGGTCACCTGGCTGGTTCGCCACACAGAGAATGTCACCAAGACAAAGACACCTGA
GGACTATGCTGACTCGAGCATTGCAGAGCTACTTTTCTTGTTGGAGGGGATTAGGTCTCTGGTCCGAAGA
CACATCAAAGTGATACAGCAATACCACCTTCAGTACTTGGCAAGATTTGATGCTCTTGTGCTCAGTGACA
TCATTCAGAACTTGTCTGTGTGTCCAGAGGAGGAGTCCATCATCATGTCCTCATTCGTCAGTATCCTCTC
CTCTCTGAATCTCAAACAAGTTGATAATGGAGAAAAATTTGAATTCTCAGGATTGAGGCTGGACTGGTTC
CGCCTACAGGCATACACTAGCGTGGCTAAGGCCCCTCTGCACCTGCATGAGAACCCTGACTTAGCCAAGG
TGATGAACCTCATTGTCTTCCACTCCCGAATGCTGGACTCCGTAGAAAAATTGCTGGTGGAAACTTCTGA
TCTGTCTACTTTCTGCTTTCATCTTCGTATCTTTGAGAAGATGTTTGCCATGACCTTGGAGGAATCTGCC
ATGTTGCGTTATGCCATTGCTTTCCCCCTGATTTGTGCTCACTTTGTCCACTGCACTCATGAGATGTGCC
CAGAGGAGTACCCCCACCTCAAGAACCATGGTCTTCACCACTGCAACTCCTTCCTGGAAGAGTTGGCCAA
GCAGACCAGCAATTGCGTCCTGGAGATCTGTGCTGAGCAGCGAAACCTGAGCGAGCAGCTTCTACCTAAG
CACTGTGCCACTACAATCAGCAAAGCCAAGAACAAGAAAACCAGGAAGCAGAGGCAGACTCCCAGAAAAG
GAGAGCCCGAGAGGGACAAGCCAGGAGCTGAGAGTCACCGGAAGAACCGCAGCATTGTCACCAACATGGA
CAAGCTACACCTAAACTTGACAGAACTGGCACTGACAATGAATCATGTATACAGTTTCTCCGTGTTTGAA
CATACTATCTTCCCTTCTGAGTACCTCAGCAGCCACCTGGAGGCCAGACTCAACAGAGCCATTGTGTGGC
TGGCTGGCTACAATGCCACGACCCAGGAGATCGTACGGCCTTCTGAGCTGTTGGCAGGAGTCAAAGCATA
CATTGGTTTCATACAGTCACTGGCCCAGTTTTTGGGTGCAGATGCTTCCAGAGTCATCCGCAACGCCCTC
CTGCAGCAGACACAACCACTGGATTCCTGTGGGGAACAGACAATCACCACACTCTACACAAACTGGTACC
TGGAAAGTCTGCTTAGACAGGCAAGCAGTGGGACCATCATCCTCTCCCCAGCCATGCAGGCCTTCGTCAG
CCTGCCCAGAGAAGGGGAGCAGAACTTCAGTGCAGAGGAGTTCTCTGACATCTCTGAGATGCGGGCCTTG
GCAGAACTCCTGGGCCCCTATGGAATGAAGTTCCTGAGTGAAAACCTGATGTGGCATGTGACCTCTCAGA
TTGTGGAGCTGAAGAAGCTGGTGGTGGAAAACATGGACATACTTGTTCAGATCAGATCCAACTTTAGCAA
GCCGGACTTGATGGCTTCCCTGCTGCCCCAGCTGACAGGGGCTGAAAATGTGCTAAAGCGCATGACCATC
ATTGGGGTTATCCTCAGTTTCAGGGCCATGGCCCAAGAGGGACTTCGGGAGGTTTTCTCCTCCCACTGCC
CATTTCTTATGGGTCCCATTGAGTGCTTGAAGGAGTTTGTCACTCCAGACACAGACATCAAGGTGACCTT
GAGTATCTTTGAGCTGGCATCTGCTGCAGGTGTGGGCTGTGACATTGACCCAGCCTTGGTGGCTGCCATT
GCTAATCTGAAAGCTGATACTTCATCTCCTGAGGAGGAATATAAGGTGGCCTGCCTGCTCTTGATCTTTC
TGGCAGTTTCCCTCCCACTCCTTGCCACTGACCCTTCTTCCTTTTATAGCATTGAGAAGGATGGTTACAA
CAACAATATTCATTGCTTGACCAAAGCCATCATCCAGGTGTCTGCTGCCCTCTTCACGCTCTACAACAAG
AACATTGAAACTCACCTCAAGGAATTTCTGGTGGTGGCCTCTGTCAGCCTCTTGCAGCTGGGCCAGGAGA
CTGACAAGCTTAAAACCAGAAATCGAGAATCCATTTCTCTGCTCATGCGCTTGGTGGTGGAGGAGTCATC
CTTCCTGACCCTGGACATGCTGGAGTCCTGTTTCCCTTATGTCCTGCTTCGAAATGCCTATCGGGAGGTG
TCTCGGGCCTTCCACCTAAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210832 representing NM_005337
Red=Cloning site Green=Tags(s)

MSLTSAYQHKLAEKLTILNDRGQGVLIRMYNIKKTCSDPKSKPPFLLEKSMEPSLKYINKKFPNIDVRNS
TQHLGPVHREKAEIIRFLTNYYQSFVDVMEFRDHVYELLNTIDACQCHFDINLNFDFTRSYLDLIVTYTS
VILLLSRIEDRRILIGMYNCAHEMLHGHGDPSFARLGQMVLEYDHPLKKLTEEFGPHTKAVSGALLSLHF
LFVRRNQGAEQWRSAQLLSLISNPPAMINPANSDTMACEYLSVEVMERWIIIGFLLCHGCLNSNSQCQKL
WKLCLQGSLYITLIREDVLQVHKVTEDLFSSLKGYGKRVADIKESKEHVIANSGQFHCQRRQFLRMAVKE
LETVLADEPGLLGPKALFAFMALSFIRDEVTWLVRHTENVTKTKTPEDYADSSIAELLFLLEGIRSLVRR
HIKVIQQYHLQYLARFDALVLSDIIQNLSVCPEEESIIMSSFVSILSSLNLKQVDNGEKFEFSGLRLDWF
RLQAYTSVAKAPLHLHENPDLAKVMNLIVFHSRMLDSVEKLLVETSDLSTFCFHLRIFEKMFAMTLEESA
MLRYAIAFPLICAHFVHCTHEMCPEEYPHLKNHGLHHCNSFLEELAKQTSNCVLEICAEQRNLSEQLLPK
HCATTISKAKNKKTRKQRQTPRKGEPERDKPGAESHRKNRSIVTNMDKLHLNLTELALTMNHVYSFSVFE
HTIFPSEYLSSHLEARLNRAIVWLAGYNATTQEIVRPSELLAGVKAYIGFIQSLAQFLGADASRVIRNAL
LQQTQPLDSCGEQTITTLYTNWYLESLLRQASSGTIILSPAMQAFVSLPREGEQNFSAEEFSDISEMRAL
AELLGPYGMKFLSENLMWHVTSQIVELKKLVVENMDILVQIRSNFSKPDLMASLLPQLTGAENVLKRMTI
IGVILSFRAMAQEGLREVFSSHCPFLMGPIECLKEFVTPDTDIKVTLSIFELASAAGVGCDIDPALVAAI
ANLKADTSSPEEEYKVACLLLIFLAVSLPLLATDPSSFYSIEKDGYNNNIHCLTKAIIQVSAALFTLYNK
NIETHLKEFLVVASVSLLQLGQETDKLKTRNRESISLLMRLVVEESSFLTLDMLESCFPYVLLRNAYREV
SRAFHLN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005337
ORF Size 3381 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005337.5
RefSeq Size 3869 bp
RefSeq ORF 3384 bp
Locus ID 3071
UniProt ID P55160
Cytogenetics 12q13.13-q13.2
Protein Pathways Regulation of actin cytoskeleton
MW 128 kDa
Summary This gene encodes a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells. The encoded protein is a part of the Scar/WAVE complex which plays an important role in regulating cell shape in both metazoans and plants. Alternatively spliced transcript variants encoding different isoforms have been found.provided by RefSeq, May 2010
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"NM_005337" in other vectors (5)
SKU Description Size Price
RC210832L3 Lenti-ORF clone of NCKAP1L (Myc-DDK-tagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1 10 ug
$1,281.00
RC210832L4 Lenti-ORF clone of NCKAP1L (mGFP-tagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1 10 ug
$1,281.00
RG210832 NCKAP1L (tGFP-tagged) - Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1 10 ug
$1,181.00
SC116794 NCKAP1L (untagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1 10 ug
$1,134.00
SC318508 NCKAP1L (untagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1 10 ug
$982.00

Plasmid Map for RC210832

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC210832
Circular map for RC210832

file-alt Citations

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