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NCKAP1L (NM_005337) Human Untagged Clone

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SKU
SC318508
NCKAP1L (untagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1
  $982.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NCKAP1L
Synonyms HEM1; IMD72
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC318508 representing NM_005337.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTCTTTGACATCTGCTTACCAGCATAAATTAGCAGAGAAGCTCACTATCCTGAATGATCGCGGTCAG
GGGGTTCTCATCCGTATGTATAACATCAAGAAGACTTGTTCAGACCCCAAATCTAAGCCACCTTTCTTA
CTGGAAAAGTCCATGGAACCATCTCTCAAGTATATCAACAAGAAATTTCCCAACATAGATGTCCGAAAC
AGCACGCAACATTTAGGACCAGTACATCGTGAAAAAGCCGAGATAATTAGATTCCTCACCAACTACTAC
CAGTCATTTGTGGATGTCATGGAATTTCGGGATCATGTATATGAACTTCTCAACACCATTGATGCCTGC
CAGTGCCATTTTGATATCAATCTCAACTTTGATTTCACTCGGAGTTACCTGGACTTGATTGTAACTTAC
ACCTCAGTCATTTTACTTCTGTCACGGATTGAAGATCGGCGGATACTCATTGGCATGTACAATTGTGCC
CATGAGATGCTGCATGGGCATGGTGACCCCAGTTTTGCCCGTCTGGGTCAGATGGTCTTGGAGTATGAC
CACCCTCTGAAGAAGCTGACAGAAGAGTTTGGGCCTCACACAAAGGCTGTGAGTGGAGCCCTCCTCTCT
TTGCATTTCCTCTTTGTCCGAAGAAACCAGGGGGCTGAGCAGTGGCGCAGTGCCCAACTTCTAAGCCTC
ATCAGCAACCCCCCAGCCATGATTAACCCTGCTAATTCAGATACAATGGCCTGTGAGTATCTGTCTGTG
GAAGTAATGGAGCGCTGGATTATCATTGGGTTTCTTCTTTGTCATGGGTGCCTCAACTCCAATAGCCAG
TGCCAGAAGCTGTGGAAGCTGTGTCTGCAGGGCTCCCTCTACATCACCCTTATCCGTGAGGATGTGCTG
CAGGTGCACAAAGTCACCGAGGACCTGTTTAGCAGTTTGAAAGGGTATGGCAAGAGAGTGGCAGACATA
AAGGAGAGCAAGGAACATGTAATTGCAAACAGTGGCCAGTTTCATTGTCAACGGCGGCAATTTCTGCGG
ATGGCAGTGAAGGAGCTGGAGACTGTGTTGGCTGATGAACCGGGACTACTGGGTCCTAAGGCTCTTTTT
GCTTTCATGGCCCTGTCCTTCATTCGTGATGAGGTCACCTGGCTGGTTCGCCACACAGAGAATGTCACC
AAGACAAAGACACCTGAGGACTATGCTGACTCGAGCATTGCAGAGCTACTTTTCTTGTTGGAGGGGATT
AGGTCTCTGGTCCGAAGACACATCAAAGTGATACAGCAATACCACCTTCAGTACTTGGCAAGATTTGAT
GCTCTTGTGCTCAGTGACATCATTCAGAACTTGTCTGTGTGTCCAGAGGAGGAGTCCATCATCATGTCC
TCATTCGTCAGTATCCTCTCCTCTCTGAATCTCAAACAAGTTGATAATGGAGAAAAATTTGAATTCTCA
GGATTGAGGCTGGACTGGTTCCGCCTACAGGCATACACTAGCGTGGCTAAGGCCCCTCTGCACCTGCAT
GAGAACCCTGACTTAGCCAAGGTGATGAACCTCATTGTCTTCCACTCCCGAATGCTGGACTCCGTAGAA
AAATTGCTGGTGGAAACTTCTGATCTGTCTACTTTCTGCTTTCATCTTCGTATCTTTGAGAAGATGTTT
GCCATGACCTTGGAGGAATCTGCCATGTTGCGTTATGCCATTGCTTTCCCCCTGATTTGTGCTCACTTT
GTCCACTGCACTCATGAGATGTGCCCAGAGGAGTACCCCCACCTCAAGAACCATGGTCTTCACCACTGC
AACTCCTTCCTGGAAGAGTTGGCCAAGCAGACCAGCAATTGCGTCCTGGAGATCTGTGCTGAGCAGCGA
AACCTGAGCGAGCAGCTTCTACCTAAGCACTGTGCCACTACAATCAGCAAAGCCAAGAACAAGAAAACC
AGGAAGCAGAGGCAGACTCCCAGAAAAGGAGAGCCCGAGAGGGACAAGCCAGGAGCTGAGAGTCACCGG
AAGAACCGCAGCATTGTCACCAACATGGACAAGCTACACCTAAACTTGACAGAACTGGCACTGACAATG
AATCATGTATACAGTTTCTCCGTGTTTGAACATACTATCTTCCCTTCTGAGTACCTCAGCAGCCACCTG
GAGGCCAGACTCAACAGAGCCATTGTGTGGCTGGCTGGCTACAATGCCACGACCCAGGAGATCGTACGG
CCTTCTGAGCTGTTGGCAGGAGTCAAAGCATACATTGGTTTCATACAGTCACTGGCCCAGTTTTTGGGT
GCAGATGCTTCCAGAGTCATCCGCAACGCCCTCCTGCAGCAGACACAACCACTGGATTCCTGTGGGGAA
CAGACAATCACCACACTCTACACAAACTGGTACCTGGAAAGTCTGCTTAGACAGGCAAGCAGTGGGACC
ATCATCCTCTCCCCAGCCATGCAGGCCTTCGTCAGCCTGCCCAGAGAAGGGGAGCAGAACTTCAGTGCA
GAGGAGTTCTCTGACATCTCTGAGATGCGGGCCTTGGCAGAACTCCTGGGCCCCTATGGCATGAAGTTC
CTGAGTGAAAACCTGATGTGGCATGTGACCTCTCAGATTGTGGAGCTGAAGAAGCTGGTGGTGGAAAAC
ATGGACATACTTGTTCAGATCAGATCCAACTTTAGCAAGCCGGACTTGATGGCTTCCCTGCTGCCCCAG
CTGACAGGGGCTGAAAATGTGCTAAAGCGCATGACCATCATTGGGGTTATCCTCAGTTTCAGGGCCATG
GCCCAAGAGGGACTTCGGGAGGTTTTCTCCTCCCACTGCCCATTTCTTATGGGTCCCATTGAGTGCTTG
AAGGAGTTTGTCACTCCAGACACAGACATCAAGGTGACCTTGAGTATCTTTGAGCTGGCATCTGCTGCA
GGTGTGGGCTGTGACATTGACCCAGCCTTGGTGGCTGCCATTGCTAATCTGAAAGCTGATACTTCATCT
CCTGAGGAGGAATATAAGGTGGCCTGCCTGCTCTTGATCTTTCTGGCAGTTTCCCTCCCACTCCTTGCC
ACTGACCCTTCTTCCTTTTATAGCATTGAGAAGGATGGTTACAACAACAATATTCATTGCTTGACCAAA
GCCATCATCCAGGTGTCTGCTGCCCTCTTCACGCTCTACAACAAGAACATTGAAACTCACCTCAAGGAA
TTTCTGGTGGTGGCCTCTGTCAGCCTCTTGCAGCTGGGCCAGGAGACTGACAAGCTTAAAACCAGAAAT
CGAGAATCCATTTCTCTGCTCATGCGCTTGGTGGTGGAGGAGTCATCCTTCCTGACCCTGGACATGCTG
GAGTCCTGTTTCCCTTATGTCCTGCTTCGAAATGCCTATCGGGAGGTGTCTCGGGCCTTCCACCTAAAC
TGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_005337
Insert Size 3384 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005337.3
RefSeq Size 3909 bp
RefSeq ORF 3384 bp
Locus ID 3071
UniProt ID P55160
Cytogenetics 12q13.13-q13.2
Protein Pathways Regulation of actin cytoskeleton
MW 128.2 kDa
Summary This gene encodes a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells. The encoded protein is a part of the Scar/WAVE complex which plays an important role in regulating cell shape in both metazoans and plants. Alternatively spliced transcript variants encoding different isoforms have been found.provided by RefSeq, May 2010
Transcript Variant: This variant (1) encodes the longer isoform (1).
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Documents
Resources
"NM_005337" in other vectors (5)
SKU Description Size Price
RC210832 NCKAP1L (Myc-DDK-tagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1 10 ug
$589.00 $981.00
RC210832L3 Lenti-ORF clone of NCKAP1L (Myc-DDK-tagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1 10 ug
$1,281.00
RC210832L4 Lenti-ORF clone of NCKAP1L (mGFP-tagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1 10 ug
$1,281.00
RG210832 NCKAP1L (tGFP-tagged) - Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1 10 ug
$1,181.00
SC116794 NCKAP1L (untagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1 10 ug
$1,134.00

file-alt Citations

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