Catalase (CAT) (NM_001752) Human Tagged ORF Clone
$491.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Catalase |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001752 |
| ORF Size | 1581 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001752.4 |
| RefSeq Size | 2300 bp |
| RefSeq ORF | 1584 bp |
| Locus ID | 847 |
| UniProt ID | P04040 |
| Cytogenetics | 11p13 |
| Domains | catalase |
| Protein Families | Druggable Genome |
| Protein Pathways | Amyotrophic lateral sclerosis (ALS), Metabolic pathways, Methane metabolism, Tryptophan metabolism |
| MW | 59.8 kDa |
| Summary | This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. provided by RefSeq, Oct 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001752" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210763L1 | Lenti ORF clone of Human catalase (CAT), Myc-DDK-tagged | 10 ug |
$791.00
|
|
| RC210763L2 | Lenti ORF clone of Human catalase (CAT), mGFP tagged | 10 ug |
$791.00
|
|
| RC210763L3 | Lenti ORF clone of Human catalase (CAT), Myc-DDK-tagged | 10 ug |
$791.00
|
|
| RC210763L4 | Lenti ORF clone of Human catalase (CAT), mGFP tagged | 10 ug |
$791.00
|
|
| RG210763 | CAT (tGFP-tagged) - Human catalase (CAT) | 10 ug |
$489.00
$691.00
|
|
| SC119081 | CAT (untagged)-Human catalase (CAT) | 10 ug |
$492.00
|
|
Citations
Catalase Overexpression Drives an Aggressive Phenotype in Glioblastoma
Flor, Oliva, Ali et al
Antioxidants (Basel) (2021) 10 (12) DOI: 10.3390/antiox10121988
Flor, Oliva, Ali et al
Antioxidants (Basel) (2021) 10 (12) DOI: 10.3390/antiox10121988
Cited by 27
AI Summary: OriGene products, specifically the mammalian expression vector with C-terminal Myc-DDK Tag (Catalog # RC210763) and the control vector (Catalog # PS100001), were used to generate U251 glioma cell lines stably overexpressing catalase, enabling the study of catalase’s role in glioblastoma aggressiveness and treatment resistance.
Snippet: mammalian vector with C-terminal Myc-DDK Tag as the control (Catalog # RC210763 and PS100001, respectively;OriGene Technologies, Rockville, MD, USA). To generate cell lines stably overexpressing CAT, cells were selected with G418 (800 µg/mL) for 15 days. The stable lines isolated were characterized for the level of CAT by Western blot analysis and enzymatic activity
M2 polarization of macrophages facilitates arsenic-induced cell transformation of lung epithelial cells
Cui, Xu, Chen et al
Oncotarget (2017) 8 (13), 21398-21409 DOI: 10.18632/oncotarget.15232
Cui, Xu, Chen et al
Oncotarget (2017) 8 (13), 21398-21409 DOI: 10.18632/oncotarget.15232
Hexavalent chromium induces malignant transformation of human lung bronchial epithelial cells via ROS-dependent activation of miR-21-PDCD4 signaling
Pratheeshkumar, Son, Divya et al
Oncotarget (2016) 7 (32), 51193-51210 DOI: 10.18632/oncotarget.9967
Pratheeshkumar, Son, Divya et al
Oncotarget (2016) 7 (32), 51193-51210 DOI: 10.18632/oncotarget.9967
L-Ascorbic acid can abrogate SVCT2-dependent cetuximab resistance mediated by mutant KRAS in human colon cancer cells
Jung, Lee, Moon et al
Free Radic. Biol. Med. (2016) 95, 200–208 DOI: 10.1016/j.freeradbiomed.2016.03.009
Jung, Lee, Moon et al
Free Radic. Biol. Med. (2016) 95, 200–208 DOI: 10.1016/j.freeradbiomed.2016.03.009
Cited by 47
AI Summary: In this study, OriGene products—specifically, human cDNA encoding SVCT-2 and myc-tagged catalase—were used to manipulate SVCT-2 and catalase expression in colon cancer cells, enabling the investigation of their roles in L-ascorbic acid and cetuximab treatment responses.
Snippet: 2.3. Plasmids, siRNA and transfection. Human cDNA encoding SVCT-2 or myc-tagged catalase was acquired from Origene (Rockville, MD, USA). We additionally constructed myc-tagged SVCT-2 from a SVCT-2-expressing plasmid using a myc-tagged pcDNA ... Cite SaveSaving
Role of reactive oxygen species in arsenic-induced transformation of human lung bronchial epithelial (BEAS-2B) cells
Zhang, Pratheeshkumar, Budhraja et al
Biochem. Biophys. Res. Commun. (2015) 456 (2), 643-8 DOI: 10.1016/j.bbrc.2014.12.010
Zhang, Pratheeshkumar, Budhraja et al
Biochem. Biophys. Res. Commun. (2015) 456 (2), 643-8 DOI: 10.1016/j.bbrc.2014.12.010
Cited by 69
Luteolin inhibits Cr (VI)-induced malignant cell transformation of human lung epithelial cells by targeting ROS mediated multiple cell signaling pathways
Pratheeshkumar, Son, Divya et al
Toxicology and Applied Pharmacology (2014)
Pratheeshkumar, Son, Divya et al
Toxicology and Applied Pharmacology (2014)
AI Summary: OriGene’s CAT-Myc-DDK-tagged plasmid was used for transfection experiments to investigate the molecular mechanisms underlying luteolin’s inhibitory effects on Cr(VI)-induced malignant transformation in human lung epithelial cells.
Snippet: GA, USA). Plasmid constructs and transfection. CAT-Myc-DDK-tagged plasmid was purchased from Origene (Rockville, MD). The SOD2-EGFP-tagged plasmid was obtained from Addgene (Cambridge, MA). Transfections were ... Cite SaveSaving
Showing 1-6 of 7 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
