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TRMT5 (NM_020810) Human Tagged ORF Clone

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SKU
RC210671
TRMT5 (Myc-DDK-tagged)-Human TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) (TRMT5)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $474.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TRMT5
Synonyms COXPD26; KIAA1393; TRM5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210671 representing NM_020810
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCTTTGGATCTTATGGAGGCCATTTGGATTCTCAGGAAGATTTCTGAAACTGGAAAGCCATAGCA
TAACTGAATCAAAATCGTTGATTCCAGTAGCTTGGACATCCCTGACACAGATGCTTTTGGAAGCACCTGG
TATTTTCTTATTGGGTCAAAGAAAAAGATTCTCAACCATGCCAGAAACAGAAACACATGAGAGAGAGACT
GAATTGTTTTCACCACCTTCTGATGTCCGAGGCATGACAAAACTTGATAGAACAGCTTTTAAAAAGACAG
TCAACATTCCAGTGCTTAAAGTGAGGAAAGAAATAGTCAGTAAATTGATGCGATCCCTAAAAAGGGCAGC
ATTGCAGCGCCCAGGCATAAGACGTGTGATTGAAGATCCGGAAGATAAAGAAAGTAGACTAATCATGTTG
GATCCCTATAAAATATTTACTCATGATTCCTTTGAGAAAGCAGAACTCAGTGTTTTAGAGCAGCTTAATG
TCAGTCCACAGATCTCTAAATACAATTTGGAACTAACATATGAACACTTTAAGTCAGAAGAAATCTTGAG
AGCTGTGCTTCCTGAAGGTCAAGATGTAACTTCAGGGTTTAGCAGGATTGGACATATTGCACACCTAAAC
CTTCGAGATCATCAGCTGCCTTTCAAACATTTAATTGGCCAGGTTATGATTGACAAAAATCCAGGAATCA
CCTCAGCAGTAAATAAAATAAATAATATTGACAATATGTACCGAAATTTCCAAATGGAAGTGCTATCTGG
AGAGCAGAACATGATGACAAAGGTTCGAGAAAACAACTACACCTATGAATTTGATTTTTCAAAAGTCTAT
TGGAATCCTCGTCTGTCTACAGAACACAGCCGTATCACAGAACTTCTCAAACCTGGGGATGTCCTATTTG
ATGTTTTTGCTGGGGTTGGGCCCTTTGCCATTCCAGTAGCAAAGAAAAACTGCACTGTATTTGCCAATGA
TCTCAATCCTGAATCTCATAAATGGCTGTTGTACAACTGTAAATTAAATAAAGTGGACCAAAAGGTGAAA
GTCTTCAACTTGGATGGGAAAGACTTCCTCCAAGGACCAGTCAAAGAAGAGTTAATGCAGCTGCTGGGTC
TGTCAAAAGAAAGAAAACCCTCTGTGCACGTTGTCATGAACTTGCCAGCAAAAGCTATAGAGTTTCTTAG
TGCTTTCAAGTGGCTTTTAGATGGGCAGCCATGCAGCAGTGAGTTCCTTCCCATAGTGCATTGTTATAGC
TTTTCCAAAGATGCTAACCCTGCTGAGGATGTTCGGCAAAGGGCTGGAGCTGTGTTAGGCATTTCTCTGG
AGGCATGCAGTTCAGTTCACCTGGTAAGAAATGTGGCCCCAAACAAGGAAATGCTGTGCATCACGTTTCA
GATTCCTGCCTCTGTCCTCTACAAGAACCAGACCAGAAATCCAGAGAATCATGAAGATCCACCTCTTAAA
AGGCAGAGGACGGCTGAAGCCTTTTCAGACGAAAAAACACAAATTGTTTCAAACACT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210671 representing NM_020810
Red=Cloning site Green=Tags(s)

MVLWILWRPFGFSGRFLKLESHSITESKSLIPVAWTSLTQMLLEAPGIFLLGQRKRFSTMPETETHERET
ELFSPPSDVRGMTKLDRTAFKKTVNIPVLKVRKEIVSKLMRSLKRAALQRPGIRRVIEDPEDKESRLIML
DPYKIFTHDSFEKAELSVLEQLNVSPQISKYNLELTYEHFKSEEILRAVLPEGQDVTSGFSRIGHIAHLN
LRDHQLPFKHLIGQVMIDKNPGITSAVNKINNIDNMYRNFQMEVLSGEQNMMTKVRENNYTYEFDFSKVY
WNPRLSTEHSRITELLKPGDVLFDVFAGVGPFAIPVAKKNCTVFANDLNPESHKWLLYNCKLNKVDQKVK
VFNLDGKDFLQGPVKEELMQLLGLSKERKPSVHVVMNLPAKAIEFLSAFKWLLDGQPCSSEFLPIVHCYS
FSKDANPAEDVRQRAGAVLGISLEACSSVHLVRNVAPNKEMLCITFQIPASVLYKNQTRNPENHEDPPLK
RQRTAEAFSDEKTQIVSNT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020810
ORF Size 1527 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020810.3, NP_065861.3
RefSeq Size 5272 bp
RefSeq ORF 1530 bp
Locus ID 57570
UniProt ID Q32P41
Cytogenetics 14q23.1
MW 58.1 kDa
Summary tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine (Brule et al., 2004 PubMed 15248782).supplied by OMIM, Mar 2008
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"NM_020810" in other vectors (6)
SKU Description Size Price
RC210671L1 Lenti ORF clone of Human TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) (TRMT5), Myc-DDK-tagged 10 ug
$774.00
RC210671L2 Lenti ORF clone of Human TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) (TRMT5), mGFP tagged 10 ug
$774.00
RC210671L3 Lenti ORF clone of Human TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) (TRMT5), Myc-DDK-tagged 10 ug
$774.00
RC210671L4 Lenti ORF clone of Human TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) (TRMT5), mGFP tagged 10 ug
$774.00
RG210671 TRMT5 (tGFP-tagged) - Human TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) (TRMT5) 10 ug
$489.00 $674.00
SC304838 TRMT5 (untagged)-Human TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) (TRMT5) 10 ug
$522.00

Plasmid Map for RC210671

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC210671
Circular map for RC210671

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