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PHF10 (NM_018288) Human Tagged ORF Clone

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SKU
RC210609
PHF10 (Myc-DDK-tagged)-Human PHD finger protein 10 (PHF10), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "PHF10 or NM_018288"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PHF10
Synonyms BAF45A; XAP135
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210609 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTCAAGAACAAGTCAGTGAATATTTGGGTGTGACCTCCTTTAAAAGGAAATATCCAGATTTAGAGC
GACGAGATTTGTCTCACAAGGAGAAACTCTACCTGAGAGAGCTAAATGTCATTACTGAAACTCAGTGCAC
TCTAGGCTTAACAGCATTGCGCAGTGATGAAGTGATTGATTTAATGATAAAAGAATATCCAGCCAAACAT
GCTGAGTATTCTGTTATTCTACAAGAAAAAGAACGTCAACGAATTACAGACCATTATAAAGAGTATTCCC
AAATGCAACAACAGAATACTCAGAAAGTTGAAGCCAGTAAAGTGCCTGAGTATATTAAGAAAGCTGCCAA
AAAAGCAGCAGAATTTAATAGCAACTTAAACCGGGAACGCATGGAAGAAAGAAGAGCTTATTTTGACTTG
CAGACACATGTTATCCAGGTACCTCAAGGGAAGTACAAAGTTTTGCCAACAGAGCGAACAAAGGTCAGTT
CTTACCCAGTGGCTCTCATCCCCGGACAGTTCCAGGAATATTATAAGAGGTACTCACCAGATGAGCTGCG
GTATCTGCCATTAAACACAGCCCTGTATGAGCCCCCTCTGGATCCTGAGCTCCCTGCTCTAGACAGTGAT
GGTGATTCAGATGATGGCGAAGATGGTCGAGGTGATGAGAAACGGAAAAATAAAGGCACTTCGGACAGCT
CCTCTGGCAATGTATCTGAAGGGGAAAGCCCTCCTGACAGCCAGGAGGACTCTTTCCAGGGAAGACAGAA
ATCAAAAGACAAAGCTGCCACTCCAAGAAAAGATGGTCCCAAACGTTCTGTACTGTCCAAGTCAGTTCCT
GGGTACAAGCCAAAGGTCATTCCAAATGCTATATGTGGAATTTGTCTGAAGGGTAAGGAGTCCAACAAGA
AAGGAAAGGCTGAATCACTTATACACTGCTCCCAATGTGAGAATAGTGGCCATCCTTCTTGCCTGGATAT
GACAATGGAGCTTGTTTCTATGATTAAGACCTACCCATGGCAGTGTATGGAATGTAAAACATGCATTATA
TGTGGACAACCCCACCATGAAGAAGAAATGATGTTCTGTGATATGTGTGACAGAGGTTATCATACTTTTT
GTGTGGGCCTTGGTGCTATTCCATCAGGTCGCTGGATTTGTGACTGTTGTCAGCGGGCCCCCCCAACACC
CAGGAAAGTGGGCAGAAGGGGGAAAAACAGCAAAGAGGGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210609 protein sequence
Red=Cloning site Green=Tags(s)

MLQEQVSEYLGVTSFKRKYPDLERRDLSHKEKLYLRELNVITETQCTLGLTALRSDEVIDLMIKEYPAKH
AEYSVILQEKERQRITDHYKEYSQMQQQNTQKVEASKVPEYIKKAAKKAAEFNSNLNRERMEERRAYFDL
QTHVIQVPQGKYKVLPTERTKVSSYPVALIPGQFQEYYKRYSPDELRYLPLNTALYEPPLDPELPALDSD
GDSDDGEDGRGDEKRKNKGTSDSSSGNVSEGESPPDSQEDSFQGRQKSKDKAATPRKDGPKRSVLSKSVP
GYKPKVIPNAICGICLKGKESNKKGKAESLIHCSQCENSGHPSCLDMTMELVSMIKTYPWQCMECKTCII
CGQPHHEEEMMFCDMCDRGYHTFCVGLGAIPSGRWICDCCQRAPPTPRKVGRRGKNSKEG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018288
ORF Size 1230 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018288.2, NP_060758.1
RefSeq Size 1692 bp
RefSeq ORF 1497 bp
Locus ID 55274
UniProt ID Q8WUB8
Cytogenetics 6q27
Domains PHD
Protein Families Druggable Genome, Stem cell - Pluripotency, Transcription Factors
MW 46.6 kDa
Summary This gene contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28. provided by RefSeq, Jul 2008
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"NM_018288" in other vectors (7)
SKU Description Size Price
RC210609L1 Lenti ORF clone of Human PHD finger protein 10 (PHF10), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC210609L2 Lenti ORF clone of Human PHD finger protein 10 (PHF10), transcript variant 1, mGFP tagged 10 ug
$757.00
RC210609L3 Lenti ORF clone of Human PHD finger protein 10 (PHF10), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC210609L4 Lenti ORF clone of Human PHD finger protein 10 (PHF10), transcript variant 1, mGFP tagged 10 ug
$757.00
RG210609 PHF10 (tGFP-tagged) - Human PHD finger protein 10 (PHF10), transcript variant 1 10 ug
$489.00 $657.00
SC111822 PHF10 (untagged)-Human PHD finger protein 10 (PHF10), transcript variant 1 10 ug
$457.00
SC327885 PHF10 (untagged)-Human PHD finger protein 10 (PHF10) transcript variant 1 10 ug
$503.00

Plasmid Map for RC210609

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC210609
Circular map for RC210609

file-alt Citations

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