c-Jun (JUN) (NM_002228) Human Tagged ORF Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | c-Jun |
| Synonyms | AP-1; AP1; c-Jun; cJUN; p39 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002228 |
| ORF Size | 993 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002228.4 |
| RefSeq Size | 3338 bp |
| RefSeq ORF | 996 bp |
| Locus ID | 3725 |
| UniProt ID | P05412 |
| Cytogenetics | 1p32.1 |
| Domains | BRLZ, Jun |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors |
| Protein Pathways | B cell receptor signaling pathway, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, GnRH signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, Pathways in cancer, Renal cell carcinoma, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Wnt signaling pathway |
| MW | 35.5 kDa |
| Summary | This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002228" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC209804L1 | Lenti ORF clone of Human jun proto-oncogene (JUN), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC209804L2 | Lenti ORF clone of Human jun proto-oncogene (JUN), mGFP tagged | 10 ug |
$600.00
|
|
| RC209804L3 | Lenti ORF clone of Human jun proto-oncogene (JUN), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC209804L4 | Lenti ORF clone of Human jun proto-oncogene (JUN), mGFP tagged | 10 ug |
$600.00
|
|
| RG209804 | JUN (tGFP-tagged) - Human jun proto-oncogene (JUN) | 10 ug |
$489.00
$500.00
|
|
| SC118762 | JUN (untagged)-Human jun proto-oncogene (JUN) | 10 ug |
$300.00
|
|
Citations
Unraveling an enhancer-silencer regulatory element showing epistatic interaction with a variant that escaped genome-wide association studies
Adjemout, Nisar, Escandell et al
Cell Genom (2025)
Adjemout, Nisar, Escandell et al
Cell Genom (2025)
Cited by 2
AI Summary: OriGene products, specifically the c-Fos (Cat#RC202597) and c-Jun (Cat#RC209804) Human Tagged ORF Clones, were used in this study to investigate the roles of these transcription factors in the regulation of the ESpromoter element affecting LAX1 expression and T cell activation.
Snippet: pGL3- Basic with 810 bp LAX1 promoter rs11240391-G allele and 610 bp ESpromoter minor haplotype This paper N/A pcDNA3.1 Mammalian expression vector ThermoFisher Cat#V79020 c Fos (FOS) (NM_005252) Human Tagged ORF Clone Origene Cat#RC202597 c-Jun (JUN) (NM_002228) Human Tagged ORF Clone Origene Cat#RC209804
Unraveling a novel dual-function regulatory element showing epistatic interaction with a variant that escapes genome-wide association studies
Adjemout, Nisar, Escandell et al
bioRxiv (2024)
Adjemout, Nisar, Escandell et al
bioRxiv (2024)
Snippet: LAX1 rs11240391-T allele or pGL3-LAX1 rs11240391-G allele with or without (3) 0.5 μg of expression plasmids coding for proteins of interest (FOS, RC202597, Origene) and or (JUN, RC209804, Origene) completed with (4) 0 to 1 μg of empty pcDNA3.1 plasmid to equalize DNA quantities as previously described66 in each condition as detailed below: 1: 0.5 μg pGL3-Renilla + 0.5 μg pGL3-LAX1-T + 1 μg pcDNA3.1 2: 0.5 μg pGL3-Renilla + 0.5 μg pGL3-LAX1-G + 1 μg pcDNA3.1 3: 0.5 μg pGL3-Renilla + 0.5
The endoplasmic reticulum acetyltransferases ATase1/NAT8B and ATase2/NAT8 are differentially regulated to adjust engagement of the secretory pathway
Rigby, Ding, Farrugia et al
J. Neurochem. (2020)
Rigby, Ding, Farrugia et al
J. Neurochem. (2020)
Cited by 16
AI Summary: OriGene products—including the anti-DDK antibody (TA50011), pCMV6-entry plasmid backbone (PS100001), and expression plasmids for human NAT8B (RC215647), NAT8 (RC203157), CREB1 (RC210577), c-FOS (RC202597), c-JUN (RC209804), and p53 (RC200003)—were used for protein detection and gene overexpression experiments that enabled the study of regulatory mechanisms controlling ATase1/NAT8B and ATase2/NAT8 in the secretory pathway.
Snippet: lysine (Abcam #ab409;RRID:AB_308707), anti-DDK (Origene TA50011;RRID:AB_2622345), and anti-Myc (Santa Cruz Biotechnology sc-40;RRID:AB_627268) (pCMV6-entry backbone;OriGene #PS100001;2017) or empty plasmid (serving as a control). … pCMV6-entry empty plasmid (OriGene, #PS100001;2017);cells were harvested 48 hr after transfection. PC12 stable lines were generated by transfecting with Lipofectamine 2000 using the following plasmids: pCMV6-entry empty plasmid (described above), human NAT8B-pCMV6 (OriGene, #RC215647;2018), or human NAT8-pCMV6 (OriGene, #RC203157;2018)...DDK (Origene, TA50011;RRID:AB_2622345)...transcription factor expression plasmid (pCMV6-entry backbone;OriGene, #PS100001;2017)...human CREB1-pCMV6 (Origene, #RC210577;2017), human c-FOS-pCMV6 (Origene, #RC202597;2017), human c-JUN-pCMV6 (Origene, #RC209804;2017), p53-pCMV6 (OriGene, #RC200003;2019)
High-resolution protein-protein interaction mapping using all-versus-all sequencing (AVA-Seq)
Andrews, Schaefer-Ramadan, Al-Thani et al
J Biol Chem (2019) 294 (30), 11549-11558 DOI: 10.1074/jbc.RA119.008792
Andrews, Schaefer-Ramadan, Al-Thani et al
J Biol Chem (2019) 294 (30), 11549-11558 DOI: 10.1074/jbc.RA119.008792
AI Summary: OriGene contributed to this study by providing six human gene clones (PKAR2 [RC220376], AKAP5 [RC221314], MDM2 [RC219518], p53 [RC200003], FOS [RC202597], and JUN [RC209804] in pCMV6-Entry vectors), which were used as source material for protein-protein interaction mapping in the AVA-Seq system.
Snippet: … Despite their success;The following six human genes were purchased from Origene as 10-μg stocks in pCMV6-Entry vector with SgfI and MluI cloning sites: PKAR2 (RC220376;1212 bp), AKAP5 (RC221314;1281 bp), MDM2 (RC219518;1491 bp), p53 (RC200003;1179 bp), FOS (RC202597;1140 bp), and JUN (RC209804;993 bp). …
Endogenous interaction profiling identifies DDX5 as an oncogenic coactivator of transcription factor Fra-1
He, Song, Sinha et al
Oncogene (2019)
He, Song, Sinha et al
Oncogene (2019)
HDAC8 regulates a stress response pathway in melanoma to mediate escape from BRAF inhibitor therapy
Emmons, Faião-Flores, Sharma et al
Cancer Res. (2019) 79 (11), 2947-2961 DOI: 10.1158/0008-5472.CAN-19-0040
Emmons, Faião-Flores, Sharma et al
Cancer Res. (2019) 79 (11), 2947-2961 DOI: 10.1158/0008-5472.CAN-19-0040
AI Summary: OriGene products contributed to this study by providing the WT c-Jun plasmid and empty vector plasmids, which were used to investigate the role of c-Jun acetylation in melanoma cell resistance to BRAF inhibitor therapy.
Snippet: The empty vector plasmids were purchased from Origene Technologies Inc (Rockville, MD) made by a site-directed mutagenesis kit (ThermoFisher) against a WT c-Jun plasmid (Origene Technologies) per manufacturers instructions. Mutant constructs were
Showing 1-6 of 16 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
